In-vitro Evaluation of Immunomodulatory Potential of Swarna Pushpa Rasa Chendhuram Using Murine Macrophage RAW 264.7 Cell Line Model

Abstract

Immunity plays an important role in the protection of the humans and animals from foreign toxic matter. In recent years, there has been growing interest in the field of siddha medicines research and in search for promising potential compounds for investigating the immunomodulatory compounds from herbomineral products. The immune system is designed to protect the host from invading pathogens and to eliminate disease. Many formulations from siddha system of medicine have been used for the purpose of increase immunity. Immunomodulatory agents of herbomineral origin increase the immune responsiveness of the body against pathogens by activating the nonspecific immune system. However, there is a need of systemic studies on siddha preparations like SwarnaPushpa Rasa Chendhuram (SPRC) to substantiate the therapeutic claims made regarding their clinical utility. Macrophage cell line RAW 264.7 are the widely utilized model for evaluating the Invitro immunomodulatory efficacy of the several siddha formulations throughout the world. The main aim of the present investigation is to evaluate the immunomodulatory activity of the siddha formulation SPRC using macrophage RAW 264.7 cell line by using lipopolysaccharide (LPS) (1µg/mL) as control. LPS induced nitrite production used an indicator for evaluating the level of phagocytosis. The concentration was measure using spectrophotometric technique at 540nm. The result obtained from the present investigation indicates that the drug SPRC exhibit significant decrease in the level of nitrites in the cell line medium from 952.38 µg to 395.50 µg. It was concluded from the result obtained from the present research work that the formulation SPRC possess promising immunomodulatory and immune enhancing potential in the tested medium.Further study regarding the isolation of active moiety responsible for immunomodulatory activity present in SPRC and its mode of action need to be determined.