In vitro evaluation of macrophage viability after incubation in orange oil, eucalyptol, and chloroform

Scelza, Míriam Zaccaro; Oliveira, Lilian Rachel Lima; Carvalho, Fernanda Blaudt; Faria, Suzana Côrte-Real

doi:10.1016/j.tripleo.2006.02.030

Cited by 39 publications

(14 citation statements)

References 15 publications

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“…[ 26 ] Further, orange oil is less cytotoxic and more biocompatible than eucalyptol and chloroform. [ 6 25 ]…”

Section: Discussionmentioning

confidence: 99%

“…However, various evidence-based studies till date have reported that filling material residues, especially sealers present on canal walls and in their microscopic ramifications, may either remain inaccessible or resist dissolution. [ 5 6 7 ] Therefore, complete removal of sealers may vary considerably. In such cases, organic solvents play a vital role in the thorough removal of endodontic sealers.…”

Section: Introductionmentioning

confidence: 99%

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The effectiveness of eucalyptus oil, orange oil, and xylene in dissolving different endodontic sealers

Yadav

Chandra

et al. 2016

J Conserv Dent

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Objective:The objective of this study was to evaluate the dissolution effectiveness of eucalyptus oil, orange oil, xylene, and distilled water on three different endodontic sealers.Materials and Methods:About 240 samples of root canal sealers (eighty for each sealer) were prepared and divided into four groups of 20 each for immersion in different organic solvents. Each group was further subdivided into two subgroups (n = 10) for 2 and 10 min of immersion time. The mean percentage of weight loss was determined for each sealer in each solvent at both time periods. Data were statistically analyzed by two factor analysis of variance and significance of mean difference was obtained by Tukey's post hoc test (P < 0.05).Results:The lowest level of solubility was observed for Adseal followed by Apexit Plus and Endomethasone N at both time periods in all solvents. Apexit Plus showed no significant (P > 0.05) difference in its dissolution in all the organic solvents except distilled water at both the time periods. The solubility profile of Endomethasone N and Adseal did not differ significantly among eucalyptus oil, orange oil, and xylene at 2 min and between eucalyptus oil and orange oil at 10 min. However, at 10 min, Endomethasone N and Adseal showed a more pronounced solubility in xylene as compared to both eucalyptus oil and orange oil.Conclusions:In general, xylene was the most effective in dissolving root canal sealers than other organic solvents. Essential oils (eucalyptus oil and orange oil) were found similar in their ability to dissolve Apexit Plus and Endomethasone N.

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“…[ 26 ] Further, orange oil is less cytotoxic and more biocompatible than eucalyptol and chloroform. [ 6 25 ]…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effectiveness of eucalyptus oil, orange oil, and xylene in dissolving different endodontic sealers

Yadav

Chandra

et al. 2016

J Conserv Dent

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“…Peritoneal exudates cells (PECs) were harvested by peritoneal lavage using chilled serum‐free culture medium RPMI 1640. The PECs were then transferred into a vented plastic tissue culture flask (Tarson) for culture at 37 °C in CO2 incubator (Shella, Oregon, CA, USA). The non‐adherent cells were discarded by washing three times with lukewarm serum‐free culture medium with gentle flushing.…”

Section: Methodsmentioning

confidence: 99%

Suppressed Expression of Homotypic Multinucleation, Extracellular Domains of CD172α (SIRP‐α) and CD47 (IAP) Receptors in TAMs UpRegulated by Hsp70–Peptide Complex in Dalton's Lymphoma

Gautam

Acharya

2014

Scand J Immunol

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CD172a and CD47 are members of glycoprotein expressed on macrophages and various immune cells, promote immune recognition and T cell stimulation that priming phagocytosis of pathogens and apoptotic bodies and malignant cell. Tumour-releasing immunosuppressive factor promotes tumour growth and transforms the tumour resident M1 phenotype of macrophage to M2 phenotype (TAMs) that promotes tumour progression by downregulating the expression of different surface receptor including CD172a and CD47. Recent studies have reported that CD172a and CD47 are involved in the pathogenesis and promote malignancies such as lymphoma, leukaemia, melanoma, lung cancer and multiple myeloma, and their expression varies during infection and malignancies. Autologous Hsp70 is well recognized for its role in activating macrophages leading to enhance production of inflammatory cytokines. It has been observed that Hsp70 derived from normal tissues do not elicit tumour immunity, while Hsp70 preparation from tumour cell was able to elicit tumour immunity. However, the role of exogenous autologous hsp70 on the formation of giant cells is completely unknown. Therefore, in the present study, we sought to investigate the effect of Hsp70-peptide complex on the expression of CD172a and CD47 receptors in normal peritoneal macrophages (NMO) and TAMs. Finding shows that the expression of CD172a and CD47 enhances in TAMs and it reverts back the suppressed function of TAMs into M1 state of immunoregulatory phenotype that promotes tumour regression by enhanced multinucleation and phagocytosis of malignant cells and significantly enhances the homotypic fusion of macrophages and polykaryon formation in vitro by enhancing the expression of SIRPa and IAP.

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“…Orange oil solvent was used as it yields the larger number of dentinal tubules free of obturating materials as compared to other solvents. [ 14 ] Ultrasonics were used as an adjunct in dislodging the remnants of obturating material using the electromagnetic energy.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of retreatment techniques by using different file systems from oval-shaped canals

Purba¹,

Sonarkar

Podar

et al. 2020

J Conserv Dent

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Introduction: Endodontic failure resolves after complete removal of obturating material followed by disinfection, cleaning, shaping and three-dimensional obturation. Materials and Methods: Seventy single-rooted mandibular premolars, with single oval-shaped root canal were included. They were decoronated at a length of 16 mm from the apex. Shaping and cleaning were performed, and obturation was done using the warm lateral compaction technique. The teeth were divided into seven groups according to retreatment procedures (Hand files; ProTaper Universal retreatment files; MTwo ® Retreatment system; R-Endo ® Retreatment system; ProTaper Universal retreatment files with solvent and ultrasonics; MTwo ® R with solvent, and ultrasonics and R-Endo ® with solvent and ultrasonics). The data were obtained and subjected to the statistical analysis. Results: All instrumentation techniques left obturating material with least in Group 6 and maximum Group 5. The mean time taken for complete retreatment procedure was minimum in Group 2 and maximum with Group 1. Conclusion: MTwo ® R with solvent and ultrasonics, R-Endo ® , and R-Endo ® with solvent and ultrasonics were effective in gutta-percha removal from coronal third, middle third, and apical third, respectively. Significance: Nonsurgical endodontic retreatment using rotary files helps in gaining access to infected teeth. During retreatment procedure, this helps disinfectant to reach in critical areas of the root canal system, thereby decreasing the microbial load, leading to achieve healthy periradicular tissues.

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In vitro evaluation of macrophage viability after incubation in orange oil, eucalyptol, and chloroform

Cited by 39 publications

References 15 publications

The effectiveness of eucalyptus oil, orange oil, and xylene in dissolving different endodontic sealers

The effectiveness of eucalyptus oil, orange oil, and xylene in dissolving different endodontic sealers

Suppressed Expression of Homotypic Multinucleation, Extracellular Domains of CD172α (SIRP‐α) and CD47 (IAP) Receptors in TAMs UpRegulated by Hsp70–Peptide Complex in Dalton's Lymphoma

Comparative evaluation of retreatment techniques by using different file systems from oval-shaped canals

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