In Vitro Evaluation of Natural Keratin Based Hydrogel from Chicken Feather Waste for Controlled Drug Release

Chandini, Sakthi D; Charulatha, M.; Legadevi, R; Meignanalakshmi, S

doi:10.20546/ijcmas.2017.610.411

Cited by 4 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FTIR analysis confirmed that the carbon rich layer we found with the EDS is composed by organic residues particularly the C-H stretch and v(C=O) peaks around 2931 and 1737 respectively (Fig. 5A), which are commonly found in collagen I (Belbachir et al 2009;Jeevithan et al 2014;Valenzuela-Rojo et al 2018) and keratin (Chandini et al 2017;Estévez-Martínez et al 2013); highly abundant proteins found in the scales and skin of fishes (Bhagwat & Dandge 2016;Elliott 2011). Amide A, I, II, and III, C-H stretch and v(C=O) peaks were clearly observed in the FTIR of the extant Orechromis sp.…”

Section: Sem-eds Resultssupporting

confidence: 65%

Peer Review #1 of "Exceptionally preserved ‘skin’ in an Early Cretaceous fish from Colombia (v0.1)"

Benton¹

2020

View full text Add to dashboard Cite

Studies of soft tissue, cells and original biomolecular constituents preserved in fossil vertebrates have increased notoriously in recent years. Here we report preservation of 'skin' with chemical and molecular characterization from a three-dimensionally preserved caudal portion of an aspidorhynchid Cretaceous fish from the equatorial Barremian of Colombia increasing the number of localities for which exceptional preservation is known. We applied several analytical techniques including SEM-EDS, FTIR and ToF-SIMS to characterize the micromorphology and molecular and elemental composition of this fossil. Here, we show that the fossilized 'skin' exhibits similarities with those from extant fish including the wrinkles after suffering compression stress and flexibility, as well as architectural and tissue aspects of the two main layers (epidermis and dermis). This similarity extends also to molecular level, with the demonstrated preservation of potential residues of original proteins not consistent with bacterial source. Our results show a potential preservation mechanism where scales may have acted as an external barrier and together with an internal phosphate layer resulting from the degradation of the dermis itself creating an encapsulated environment for the integument.

show abstract

Section: Sem-eds Resultssupporting

confidence: 65%

Peer Review #1 of "Exceptionally preserved ‘skin’ in an Early Cretaceous fish from Colombia (v0.1)"

Benton¹

2020

View full text Add to dashboard Cite

show abstract

“…FTIR analysis confirmed that the carbon rich layer we found with the EDS is composed of, organic residues, particularly the C-H stretch and v(C=O) peaks around 2,931 and 1,737 respectively (Fig. 5A), which are commonly found in collagen I (Belbachir et al, 2009;Jeevithan et al, 2014;Valenzuela-Rojo, López-Cervantes & Sánchez-Machado, 2018) and keratin (Chandini et al, 2017;Estévez-Martínez et al, 2013); highly abundant proteins found in the scales and skin of fishes (Bhagwat & Dandge, 2016;Elliott, 2011). Amide A, I, II, and III, C-H stretch and v(C=O) peaks were clearly observed in the FTIR of the extant Orechromis sp.…”

Section: Systematic Paleontologysupporting

confidence: 66%

Exceptionally preserved ‘skin’ in an Early Cretaceous fish from Colombia

Alfonso-Rojas

Cadena

2020

PeerJ

View full text Add to dashboard Cite

Studies of soft tissue, cells and original biomolecular constituents preserved in fossil vertebrates have increased greatly in recent years. Here we report preservation of ‘skin’ with chemical and molecular characterization from a three-dimensionally preserved caudal portion of an aspidorhynchid Cretaceous fish from the equatorial Barremian of Colombia, increasing the number of localities for which exceptional preservation is known. We applied several analytical techniques including SEM-EDS, FTIR and ToF-SIMS to characterize the micromorphology and molecular and elemental composition of this fossil. Here, we show that the fossilized ‘skin’ exhibits similarities with those from extant fish, including the wrinkles after suffering compression stress and flexibility, as well as architectural and tissue aspects of the two main layers (epidermis and dermis). This similarity extends also to the molecular level, with the demonstrated preservation of potential residues of original proteins not consistent with a bacterial source. Our results show a potential preservation mechanism where scales may have acted as an external barrier and together with an internal phosphate layer resulting from the degradation of the dermis itself creating an encapsulated environment for the integument.

show abstract