In Vitro Evaluation of NLS-DTX Activity in Triple-Negative Breast Cancer

Abstract

Cancer is one of the most lethal diseases in the world, and the development and improvement of treatments used in cancer therapies are extremely important for a better quality of life for patients. In view of the current problems in drug administration such as low solubility and adverse effects, the activity of a solid lipid nanoparticle containing docetaxel (SLN-DTX), a drug already used in conventional therapies, was evaluated in a cell line (MDA-MB-231) of one of the most aggressive types of breast cancer with the worst prognosis, triple-negative breast cancer. Viability tests indicated that SLN-DTX has a greater dependence on the treatment dose when compared to the free drug, which indicates a more controlled release of the drug, and both reduced viability by around 50% at a concentration of 1 µg/mL after 72 h. Transmission electron microscopy (TEM) and confocal and light microscopy analyses indicated that after treatment the cells enter a mitotic catastrophe, characteristic of antimitotic drugs that usually make cells progress to death or senescence. Cells treated with both DTX and SLN-DTX showed significant inhibition of mobility, 73.6% and 66.5% when treated with SLN-DTX and DTX, respectively, compared to the 11.4% of the control after 72 h, characteristics that are very relevant in tumor development and progression. SLN-DTX demonstrated its great potential as a nanocarrier by maintaining and improving the drug’s action in the MDA-MB-231 cell line.

Potential Biological Properties of Lycopene in a Self-Emulsifying Drug Delivery System

“…When treated with the free LPG, the presence of structures involved in the endocytosis process is accentuated, mainly in autophagy structures ( Figure 5 C). The interaction of the nanoemulsion in different cell types is already present in the literature, mainly in the description of the use of the nanoemulsion as a drug carrier in cellular models of breast cancer [ 50 , 51 ]. TEM images also showed evidence of the interaction and internalization of nanoLPG into the endosome in human prostate cancer cells (DU-145) [ 17 ].…”