In Vitro Evaluation of Osteoblast Response to the Effect of Injectable Platelet-rich Fibrin Coating on Titanium Disks

Shah, Radhika; Thomas, Raison; Gowda, Triveni M; Baron, Tarun Kumar; Vemanaradhya, Gayathri Gunjiganur; Bhagat, Shivani

doi:10.5005/jp-journals-10024-3039

Cited by 10 publications

(9 citation statements)

References 16 publications

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“…Thus, the study does not present the variation of patients that may benefit from the use of liquid PRF to prepare sticky bone. Secondly, we only applied one centrifugation time of 8 min that is clinically applicable, but we did not cover the wide spectrum of possible protocols that include short centrifugation times of as little as 3 min [ 27 ]. Thirdly, there are more methods available to measure the concentration and particular rate of clotting of the fibrinogen such as other immunological fibrinogen assays including radial immunodiffusion and immunonephelometric [ 21 ] or clotting rate assays [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Fibrinogen Concentrations in Liquid PRF Using Various Centrifugation Protocols

et al. 2022

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Liquid platelet-rich fibrin (PRF) is produced by fractionation of blood without additives that initiate coagulation. Even though liquid PRF is frequently utilized as a natural source of fibrinogen to prepare sticky bone, the concentration of fibrinogen and the overall amount of “clottable PRF” components have not been evaluated. To this aim, we prepared liquid PRF at 300, 700, and 2000 relative centrifugal force (RCF), for 8 min and quantified the fibrinogen levels by immunoassay. We report here that, independent of the RCF, the fibrinogen concentration is higher in the platelet-poor plasma (PPP) compared to the buffy coat (BC) fraction of liquid PRF and further decreases in the remaining red fraction. We then determined the weight of the clotted PRF fractions before and after removing the serum. The PPP and BC fractions consist of 10.2% and 25.3% clottable matrix suggesting that more than half of the weight of clottable BC is caused by cellular components. Our data provide insights into the distribution of fibrinogen in the different fractions of liquid PRF. These findings suggest that PPP is the main source of clottable fibrinogen, while the BC is more a cell source when it comes to the preparation of sticky bone.

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Section: Discussionmentioning

confidence: 99%

Fibrinogen Concentrations in Liquid PRF Using Various Centrifugation Protocols

et al. 2022

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“…1 E). PRP was selected as a source of blood-borne factors because PRP is derived from the blood of each individual and contains high concentrations of various osteogenic cytokines [ 25 – 28 ]. RhBMP-2 was selected to represent one of the existing bone-derived factors, despite not being from the experimental animals in this study, because bone morphogenetic proteins, BMP-2 in particular, are highly osteoinductive growth factors released from old bone matrix, and a previous immunohistochemical analysis of bone samples surrounding Ti implants revealed high levels of BMP-2 [ 11 , 13 , 29 – 32 ].…”

Section: Methodsmentioning

confidence: 99%

Platelet-rich plasma alone is unable to trigger contact osteogenesis on titanium implant surfaces

et al. 2022

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Purpose Osseointegration consists of bidirectional bone formation around modified implant surfaces by contact osteogenesis and distance osteogenesis. This study tested whether contact osteogenesis on the surface of a modified titanium (Ti) implant is stimulated by cytokines in the blood. Methods In the first two types of experiments, sandblasted, large-grit, acid-etched Ti implants and turned Ti tubes were inserted into rabbit tibiae. To exclude the influence of distance osteogenesis, the tubes were inserted into the tibiae, and implants were placed inside the tubes. In a third type of experiment, the implants and tubes were inserted into the rabbit tibiae, and platelet-rich plasma (PRP) or recombinant human bone morphogenetic protein-2 (rhBMP-2) was applied topically. Four weeks after implantation, undecalcified specimens were prepared for histomorphometry. Bone-to-implant contact (BIC) and bone area per tissue (BA) were measured, and the data were analysed using one-way ANOVA at a significance level of 0.05. Results When the response of bone to Ti tubes with implants was compared to that without implants (first experiment), little bone formation was found inside the tubes. The mean BIC of implant specimens inside the tubes was 21.41 ± 13.81% in a second experiment that evaluated bone responses to implants with or without Ti tubes. This mean BIC value was significantly lower than that in the implant-only group (without tubes) (47.32 ± 12.09%, P = 0.030). The third experiment showed that rhBMP-2 significantly increased contact osteogenesis on the implant surface, whereas PRP had no effect (mean BIC: 66.53 ± 14.06% vs. 16.34 ± 15.98%, P = 0.004). Conclusions Platelet-rich plasma alone is unable to trigger contact osteogenesis on the modified titanium implant surface.

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“…Recently, various composites of platelet-rich fibrin (PRF) with other biomaterials have been developed in making new regenerative bone materials (66)(67)(68)(69)(70)(71)(72)(73)(74)(75)(76)(77)(78)(79). Folded PRF with human cortical bone matrix gelatin has shown better resistance against proteolytic digestion which is important in reducing the degradation rate of the bone matrices (66).…”

Section: Development Of Fibrin Composite In Mimicking Bone Tissuementioning

confidence: 99%

“…Besides, a combination of PRF, silicon and autologous bone has sped up the in-vivo bone mineralization (67). Another interesting approach is the combination of PRF with alloy materials such as Magnesium (Mg) and Titanium that has enhanced the in vitro bone mineralization (68,69). By combining with recombinant human bone morphogenetic protein 2 (rhBMP-2), PRF in Titanium mesh has improved the quality and quantity of bone formation in humans with vertical maxillary defects (70).…”

Section: Development Of Fibrin Composite In Mimicking Bone Tissuementioning

confidence: 99%

“…By combining with recombinant human bone morphogenetic protein 2 (rhBMP-2), PRF in Titanium mesh has improved the quality and quantity of bone formation in humans with vertical maxillary defects (70). The integration of fibrin with alloy materials that have shown good osteogenic differentiation properties will be beneficial to the development of functional bone implants in bone regeneration (68)(69)(70)(71).…”

Section: Development Of Fibrin Composite In Mimicking Bone Tissuementioning

confidence: 99%

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Angiogenic and Osteogenic Properties of Fibrin in Bone Tissue Engineering

Maaruf¹,

Jusoh²

2022

MJMHS

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The bone scaffold has become a promising alternative in bone tissue engineering due to the limitation associated with current bone treatments. However, the selection of scaffold material that could accurately mimic the extracellular matrix of native tissue remains challenging. Owing to its biological origin properties, natural materials including fibrin are widely used as scaffold materials as compared to synthetic materials. Fibrin has been recognized as one of the appealing natural biopolymers, which possesses unique characteristic due to its natural formed nano-scaffold, which provide a temporary matrix that facilitates cellular activities of cells. Fibrin has shown remarkable effects over other biomaterials in inducing angiogenesis and osteogenesis in bone regeneration owing to its mechanical and biological properties. In this article, we highlight the significance of fibrin materials in facilitating bone regeneration. We focus on the manipulation of fibrin composition and on the recent developments of fibrin composites in enhancing osteogenesis and angiogenesis for bone healing.

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In Vitro Evaluation of Osteoblast Response to the Effect of Injectable Platelet-rich Fibrin Coating on Titanium Disks

Cited by 10 publications

References 16 publications

Fibrinogen Concentrations in Liquid PRF Using Various Centrifugation Protocols

Fibrinogen Concentrations in Liquid PRF Using Various Centrifugation Protocols

Platelet-rich plasma alone is unable to trigger contact osteogenesis on titanium implant surfaces

Angiogenic and Osteogenic Properties of Fibrin in Bone Tissue Engineering

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