In Vitro Evaluation of Probiotic Potential and Safety Assessment of Lactobacillus mucosae Strains Isolated from Donkey’s Lactation

Rastogi, Sonakshi; Mittal, Vineeta; Singh, Aditi

doi:10.1007/s12602-019-09610-0

Cited by 42 publications

(22 citation statements)

References 58 publications

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“…Microbes in milk have been widely reported, but the majority of probiotics in milk samples were also found in human milk or bovine milk [24]. These microorganisms derived from breast milk have the advantage of direct vertical transmission which deserves more in-depth study.…”

Section: Introductionmentioning

confidence: 99%

Functional probiotics of lactic acid bacteria from Hu sheep milk

Chen

Wang

et al. 2020

BMC Microbiol

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Background: Probiotics are being considered as valuable microorganisms related to human health. Hu sheep is referred as one of the important sheep breeds in China. Goat milk produced by Hu sheep is characterized with high nutritional value and hypoallergenic in nature. Particularly, this milk contains plenty of milk prebiotic and probiotic bacteria. This study was aimed to scrutinize more bacterial strains from Hu sheep milk with potential probiotic activity. Results: Based on 16S rRNA sequence analysis, pool of forty bacterial strains were identified and evaluated their antimicrobial activities against Staphylococcus aureus, enterohemorrhagic Escherichia coli (EHEC), Salmonella typhimurium, Listeria monocytogenes enterotoxigenic E. coli (ETEC) and Aeromonas caviae. Four out of these isolated strains demonstrated their efficient bacteriostatic ability and potential healthy properties. We also examined the safety aspects of these bacterial candidates including three Lactococcus lactis strains (named as HSM-1, HSM-10, and HSM-18) and one Leuconostoc lactis strain (HSM-14), and were further evaluated via in vitro tests, including antimicrobial activity, cell surface characteristics (hydrophobicity, co-aggregation, and self-aggregation), heat treatment, antibiotic susceptibility, simulated transport tolerance in the gastrointestinal tract, and acid/bile tolerance. The obtained results revealed that HSM-1, HSM-10, HSM-14, and HSM-18 showed high survival rate at different conditions for example low pH, presence of bovine bile and demonstrated high hydrophobicity. Moreover, HSM-14 had an advantage over other strains in terms of gastrointestinal tract tolerance, antimicrobial activities against pathogens, and these results were significantly better than other bacterial candidates.

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Section: Introductionmentioning

confidence: 99%

Functional probiotics of lactic acid bacteria from Hu sheep milk

Chen

Wang

et al. 2020

BMC Microbiol

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“…Gelatinase production was examined as described by Rastogi et al [28]. Briefly, freshly grown culture of L. gasseri ATCC 33323 was spotted on MRS agar containing 5% (w/v) gelatin (Sigma-Aldrich, USA) and incubated anaerobically at 37 °C for 72 h. The plates were then allowed to stand at 4 °C for an additional 4 h. Thereafter, development of an opaque halo around the spots indicated a positive reaction.…”

Section: Gelatinase Activitymentioning

confidence: 99%

“…Susceptibility profile of L. gasseri ATCC 33323 to a selection of antibiotics was determined by Kirby-Bauer disc diffusion method as previously described [28]. The following antibiotic discs (Rosco Diagnostica, Taastrup, Denmark) were used: amikacin (30 μg), gentamycin (10 μg), ampicillin (10 μg), amoxicillin (10 μg), oxacillin (1 μg), vancomycin (30 μg), linezolid (30 μg), chloramphenicol (30 μg), tetracycline (30 μg), doxycycline (30 μg), erythromycin (10 μg), ciprofloxacin (5 μg), cephalothin (30 μg), cefotaxime (30 μg), imipenem (10 μg), and clindamycin (2 μg).…”

Section: Antibiotic Susceptibility Testingmentioning

confidence: 99%

Effects of a Potential Probiotic Strain Lactobacillus gasseri ATCC 33323 on Helicobacter pylori-Induced Inflammatory Response and Gene Expression in Coinfected Gastric Epithelial Cells

Yarmohammadi

Yadegar

Ebrahimi

et al. 2020

Probiotics & Antimicro. Prot.

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In the present study, we aimed to investigate the modulatory effects of a potential probiotic bacterium Lactobacillus gasseri ATCC 33323 on Helicobacter pylori-induced inflammatory response and gene expression in human gastric adenocarcinoma (AGS) cell line. The gastric epithelial cells were coinfected with a collection of H. pylori clinical strains alone or in combination with L. gasseri at a multiplicity of infection (MOI) of 1:100 for each bacterium, and incubated for different time points of 3, 6, and 12 h. IL-8 secretion from coinfected AGS cells after incubation at each time point was measured by an enzyme-linked immunosorbent assay (ELISA). The mRNA expression of IL-8, Bcl-2, β-catenin, integrin α 5 , and integrin β 1 genes was determined by quantitative RT-PCR amplification of total RNA extracted from coinfected epithelial cells. L. gasseri significantly (P < 0.05 and P < 0.01) decreased the production of IL-8 in AGS cells coinfected with H. pylori strains at 6 h post-infection. We also detected that L. gasseri significantly (P < 0.05) down-regulated the gene expression level of IL-8 in H. pylori-stimulated AGS cells after 6 and 12 h of coinfection. Similarly, L. gasseri caused a significant decrease (P < 0.05) in mRNA expression of Bcl-2, β-catenin, integrin α 5 , and integrin β 1 genes in AGS cells at 3 and 6 h after infection with H. pylori strains as compared with non-infected control cells. In conclusion, our results demonstrated that L. gasseri ameliorates H. pylori-induced inflammation and could be developed as a supplementation to the current treatment regimens administrated against H. pylori infection.

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“…Antimicrobial activity of Lact. gasseri strains against seventeen toxic enteric pathogens was determined as described by Rastogi et al, (2019) 15 . Both isolates were anaerobically cultured in MRS broth for 48 h, followed by centrifugation at 12000xg for 15 min at 4°C.…”

Section: Antagonistic Activitymentioning

confidence: 99%

In vitro Assessment of Antioxidant and Antimicrobial Potential of Lactobacillus gasseri Strains Isolated from Human Milk and Infant Faeces

Rastogi¹,

Mittal²,

Singh³

2020

J Pure Appl Microbiol

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The present work demonstrates the antagonistic and antioxidant property of Lactobacillus gasseri lGs22 (MN258931), Lactobacillus gasseri MVS25(MN396621) isolated from human milk and Lactobacillus gasseri LBM220 (MN097539) isolated from exclusively breast-fed infant faeces. Antagonistic potential was assayed using agar well diffusion method against 17 food-borne and enteric pathogens. Further, in order to characterize the nature of inhibitory metabolites in culture supernatant, several preliminary experiments such as pH adjustment,treatment with protease, catalase and heat were performed. In addition, quantitative estimation of antimicrobial activity was performed using Time-kill assay against indicator pathogen Sh. flexneri type 2. Strains were further evaluated for antioxidant action such as scavenging of hydroxyl radical, DPPH radical and ABTS + radical. The capacity to resist 0.4 mM, 0.8mM and 1 mM hydrogen peroxide for 16 h was also studied. Results report the efficient inhibitory potential of Lact. gasseri strains with zone sizes ranging from moderate to very strong inhibition (10-22 mm) against most of the indicator pathogens employed in the current study. Also, inhibitory action of cell-free culture supernatant of isolates LBM220 and MVS25 when adjusted to pH 6.5 was significantly reduced, elucidating the production of organic acids, while isolate LGS22 produced hydrogen peroxide as inhibitory metabolite. Lact. gasseri isolates were found to significantly scavenge free radicals, with scavenging potential in range of (80.687-26.552%). It is concluded that these three Lact. gasseri isolates may be explored more for their bio-potential as food additive with antioxidant and antimicrobial activities.

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In Vitro Evaluation of Probiotic Potential and Safety Assessment of Lactobacillus mucosae Strains Isolated from Donkey’s Lactation

Cited by 42 publications

References 58 publications

Functional probiotics of lactic acid bacteria from Hu sheep milk

Functional probiotics of lactic acid bacteria from Hu sheep milk

Effects of a Potential Probiotic Strain Lactobacillus gasseri ATCC 33323 on Helicobacter pylori-Induced Inflammatory Response and Gene Expression in Coinfected Gastric Epithelial Cells

In vitro Assessment of Antioxidant and Antimicrobial Potential of Lactobacillus gasseri Strains Isolated from Human Milk and Infant Faeces

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