In vitro evaluation of the neuroprotective potential of Olea dioica against Aβ peptide-induced toxicity in human neuroblastoma SH-SY5Y cells

Abstract

Alzheimer’s disease (AD) is a type of neurodegenerative disease, associated with the hastening of ROS, acetylcholinesterase (AChE) activity, and amyloid β peptides plaques in the brain. The limitations and side effects of existing synthetic drugs incline toward natural sources. In the present communication active principles of methanolic extract of Olea dioica Roxb, leaves are explored as an antioxidant, AChE inhibitor, and anti-amyloidogenic. Furthermore, neuroprotection against the amyloid beta-peptide has been studied. The bioactive principles were identified by GC-MS and LC-MS and further subjected to antioxidant (DPPH and FRAP) and neuroprotection (AChE inhibition, ThT binding, and MTT assay, DCFH-DA and lipid peroxidation (LPO) assay using neuroblastoma (SHSY-5Y) cell lines) assays. Methanolic extract of O. dioica Roxb, leaves was found to contain polyphenols and flavonoids. In vitro assays exhibited potential antioxidant and anti-AChE (˃50%) activities. ThT binding assay indicated protection against amyloid-beta aggregation. MTT assay, Aβ1-40 (10 µM) with extract increase the cell viability (˃50%) and showed significant cytotoxicity to SHSY-5Y cells. ROS level (˃25%) significantly decreased in the Aβ1-40 (10 µM) + extract (15 and 20 μM/mL) and LPO assay (˃50%) suggesting prevention of cell damage. Results advocate that O. dioica leaves are a good source of antioxidants, anti-AChE, and anti-amyloidogenic compounds which may be further evaluated as a natural medicine for the treatment of AD.

A Comprehensive Analytical Review of Polyphenols: Evaluating Neuroprotection in Alzheimer’s Disease

“…In animal models, it has been used to evaluate studies with AChE and BChE and antioxidant capacity [ 122 ] and in studies of behavioral difference in treated and untreated animals [ 123 , 124 ]. Studies of measures of antioxidant capacity in human neuroblastoma trials have evaluated the difference between groups treated with dioic oleic extracts and control groups [ 125 ].…”

An overview of some pharmacologically relevant therapeutic plants from Kerala's sacred groves

“…Osbeckia muralis's preliminary phytochemical contents include flavonoids, tannins, and terpenoids [41] . Through GC-MS analysis, active chemical compounds derived from Olea dioica include lupeol tri-fluoroacetate, octadecenoic acid, and hexadecanol [42] . Alkaloids, cardiac glycosides, tannins, saponins are preliminary phytoconstituents from Panicum maximum that attest to its antiinflammatory activity [43] .…”