In Vitro Evaluation of the Effects of Low‐Intensity Nd:YAG Laser Irradiation on the Inflammatory Reaction Elicited by Bacterial Lipopolysaccharide Adherent to Titanium Dental Implants

Giannelli, Marco; Bani, Danièle; Tani, Akio; Pini, Alessandro; Margheri, Martina; Zecchi‐Orlandini, Sandra; Tonelli, P; Formigli, Lucia

doi:10.1902/jop.2009.080648

Cited by 37 publications

(38 citation statements)

References 30 publications

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“…Recently, laser therapy, such as with high-level erbium:yttrium-aluminium-garnet (Er:YAG) and neodymium:yttrium- aluminium-garnet (Nd:YAG) lasers, has been introduced in periodontal therapy, because dental lasers display several advantages that may be valuable in managing periodontal disease in addition to their anti-inflammatory effects [8,9,10,11,12,13,14,15]. Previous studies have reported that Er:YAG laser irradiation has beneficial effects, including calculus removal, high bactericidal effect, and potential biostimulation [16,17,18,19,20,21] and lower pulse energy Nd:YAG laser irradiation resulted in effective in eradication of both aerobic and anaerobic microorganisms as well as in neutralizing pro-inflammatory molecules such as lipopolysaccharide [22,23,24]. In recent years, in vitro and in vivo studies have reported that photodynamic therapy, combining low-energy laser light with photosensitizing compounds that bind to target cells, is useful for eradicating bacteria from periodontal pockets [23,25,26,27].…”

Section: Introductionmentioning

confidence: 99%

Electromagnetic Wave Irradiation Promotes Osteoblastic Cell Proliferation and Up-Regulates Growth Factors via Activation of the ERK1/2 and p38 MAPK Pathways

Yumoto

Hirao²,

Tominaga³

et al. 2015

Cell Physiol Biochem

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Background/Aims: Periodontitis with bone resorption is caused by inflammatory reactions to bacterial infection. We recently reported that electromagnetic wave irradiation (EMWI) has bactericidal effects. However, the effects of EMWI on periodontal tissues remain unclear. This study was aimed to investigate the effects of EMWI on osteoblasts. Methods: Osteoblastic cells MC3T3-E1 were treated with EMWI (500-1,000 kHz, 5 times, 1 sec/time). Cell growth and cytotoxicity were determined by cell proliferation assays and measurement of lactate dehydrogenase release, respectively. Gene expression and protein production of growth factors were analyzed using real-time PCR and ELISA, respectively. EMWI-activated cellular signal transduction pathways were investigated by immunoblotting and blocking assay with specific inhibitors. Results: Osteoblasts proliferation was significantly enhanced 3 days after EMWI and no cytotoxicity was observed. EMWI up-regulated various growth factors, such as vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF). EMWI induced ERK1/2, p38 MAPK and SAPK/JNK phosphorylation within 5 min, and the production of PDGF-ΑΑ and VEGF was partially reduced by MAPK-specific inhibitor. Conclusion: These findings demonstrated that EMWI increases osteoblastic cell activity and the expression of growth factors via ERK1/2 and p38 MAPK pathways and suggested that EMWI may be beneficial to bone tissue repair such as periodontitis.

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Section: Introductionmentioning

confidence: 99%

Electromagnetic Wave Irradiation Promotes Osteoblastic Cell Proliferation and Up-Regulates Growth Factors via Activation of the ERK1/2 and p38 MAPK Pathways

Yumoto

Hirao²,

Tominaga³

et al. 2015

Cell Physiol Biochem

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“…The bactericidal and biostimulant properties of Nd:YAG laser, used in a non-focused way, have been reported in the literature: Nd:YAG laser device showed its bactericidal properties using relatively low parameters (1.5 W, 15 Hz) in non contact mode, but also provided experimental evidence for its role at low pulse energy and in promoting proliferation and differentiation of several cell types representative of the oral microenvironement (2-4). …”

Section: Introductionmentioning

confidence: 99%

Thermal increase in the oral mucosa and in the jawbone during Nd:YAG laser applications. Ex vivo study

Vescovi

Merigo

Fornaini³

et al. 2012

Med Oral

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Objective: Literature reports bactericidal and biostimulant effects for Nd:YAG laser procedures on bone and oral mucosa but the possible overheating can cause damage to anatomical structures. The aim of the study is the evaluation of thermal increase in different levels of oral tissues: mucosa, periosteum and bone during defocused application of Nd:YAG laser at different parameters. Study Design: Superficial thermal evaluation was performed in pig jaws with a thermal camera device; deep thermal evaluation was realized by 4 thermocouples placed at a subperiosteal level and at 1,2 and 4 mm depth in the jaw bone. Laser applications of 1 minute were performed 5 times (with a pause of 1 minute) on a surface of 4 cm2 with a Nd:YAG laser (VSP mode, 320 micrometer fiber, defocused mode) with different parameters. Temperatures were recorded before and after laser applications and after each pause in order to evaluate also the thermal relaxation of tissues. Results: At submucosal level, mean thermal increase was between 1.1°C and 13.2°C, at 1 mm depth between 1.1°C and 8.5°C, at 2 mm depth between 1.1°C and 6.8°C, at 4 mm depth between 1.0°C and 5.3°C. Temperature decrease during the rest time period was variable between 0°C and 2.5°C. Conclusions: Temperatures reached during clinical procedures with parameters reported in the literature in biostimulation protocols (1.25-2 Watts) for the five minutes of application are not dangerous for biological structures. The decrease in temperature during the rest time period is less considerable in the bone in comparison to oral mucosa. Key words:Nd:YAG laser, thermal increase, thermocouple, thermal camera, low level laser therapy.

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“…After reviewing the titles and abstracts, 26 papers were further evaluated for eligibility with full text. Seventeen articles 5,8,26‐40 were excluded, and the reasons for exclusion are listed in http://www.joponline.org/doi/suppl/10.1902/jop.2014.130620/suppl_file/130620_suptab1.pdf in online Journal of Periodontology .…”

Section: Resultsmentioning

confidence: 99%

Clinical Outcomes of Using Lasers for Peri‐Implantitis Surface Detoxification: A Systematic Review and Meta‐Analysis

Mailoa

Lin

Chan

et al. 2014

Journal of Periodontology

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Seven human prospective clinical trials and two animal studies were included. In four and three human studies, lasers were accompanied with surgical and non-surgical treatments, respectively. The meta-analyses showed an overall weighted mean difference of 0.00 mm (95% confidence interval = -0.18 to 0.19 mm) PD reduction between the laser and conventional treatment groups (P = 0.98) for non-surgical intervention. In animal studies, laser-treated rough-surface implants had a higher percentage of bone-to-implant contact than smooth-surface implants. In a short-term follow-up, lasers resulted in similar PD reduction when compared with conventional implant surface decontamination methods.

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In Vitro Evaluation of the Effects of Low‐Intensity Nd:YAG Laser Irradiation on the Inflammatory Reaction Elicited by Bacterial Lipopolysaccharide Adherent to Titanium Dental Implants

Abstract: By blunting the LPS-induced inflammatory response, Nd:YAG laser irradiation may be viewed as a promising tool for the therapeutic management of peri-implantitis.

Cited by 37 publications

References 30 publications

Electromagnetic Wave Irradiation Promotes Osteoblastic Cell Proliferation and Up-Regulates Growth Factors via Activation of the ERK1/2 and p38 MAPK Pathways

Electromagnetic Wave Irradiation Promotes Osteoblastic Cell Proliferation and Up-Regulates Growth Factors via Activation of the ERK1/2 and p38 MAPK Pathways

Thermal increase in the oral mucosa and in the jawbone during Nd:YAG laser applications. Ex vivo study

Clinical Outcomes of Using Lasers for Peri‐Implantitis Surface Detoxification: A Systematic Review and Meta‐Analysis

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