In vitro evaluation of the efficacy of peach stones as mycotoxin binders

Abstract

The paper describes in vitro model for the evaluation of ability of peach shell (unmodified and modified), prepared at the Institute for Technology of Nuclear and Other Mineral Raw Materials, Belgrade, to adsorb different mycotoxins. Peach stones were obtained from “Vino Župa” Company from Aleksandrovac, where they have been disposed of as by-products from their Juice Factory. After proper preparation, two sorts of peach shell particles were used: one as unmodified peach shell particles (PS) and another one obtained by acid modification, denoted as MPS. Adsorption of six mycotoxins: aflatoxin B1 (AFL), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZON), diacetoxyscirpenol (DAS) and T-2 toxin by PS and MPS was tested in vitro. Crude extracts of mycotoxins, produced at the Department of Microbiology of Bio-ecological Center, Zrenjanin, were used for adsorption experiments. The ability of binding mycotoxins was evaluated in the electrolyte 0.1 M K2HPO4, which pH value was adjusted to 3.0 and 7.0, respectively. Mass ratio of individual mycotoxin and peach shell samples was 1:5000. The experimental mixtures were incubated for 1 hour on a rotary shaker (185 rpm) at room temperature (22-25ºC). After incubation, the extractions of non-adsorbed mycotoxins from the filtrates were performed with organic solvents, and their quantification was done by thin-layer chromatography (TLC). [Projekat Ministarstva nauke Republike Srbije, br. TR 31003: Development of technologies and products based on mineral raw materials and waste biomass for protection of natural resources for safe food production i br. TR 31023: Reduction of toxigenic Fusarium species and their mycotoxins in production of safe cereal-based foods

Potential of Different Mycotoxin Adsorbents Under in Vitro Conditions

“…However, there were certain diff erences in the experimental design that could produce a diff erent result (the ratio of individual mycotoxins and adsorbents in this research was 1:5000 and the particle size of the investigated biomass < 100 μm). Comparable research was conducted by Lopičić et al (2013), at pH values of 3.0 and 7.0. Similar to the previous study, the best adsorption index of peach stones was obtained for afl atoxin (58.82% at both pH values), the poorest for DAS (0% and 16.67%), while for OTA it was 66.67% and 19.98% and 23.08% and 39.97% for DON, at pH 3.0 and 7.0, respectively.…”

“…Its use is already permitted in animal feed at up to 2% to prevent ruminal acidosis in ruminants (US Food and Drug Administration, 2019; Adamović et al, 2020). Another potential adsorbent is a preparation of ground peach stones (Lopičić et al, 2013). It was prepared in two fractions: larger (particle size d = 0.1 mm) and smaller (particle size d < 0.1 mm) fraction.…”

Management of waste biomass from food industry: Potential application of peach shells for waste water treatment

“…Beside methylene blue dye and heavy metals, powdered peach shell particles supplied from Vino Župa, Aleksandrovac, Serbia, have been tested as biosorbent of mycotoxins (Adamović et al, 2013;Lopičić et al, 2013b). The obtained results indicate that peach shell particles can be successfully applied as effective biosorbents of mycotoxins, while pretreatment of the particles by acid modification leads to the improvement of their biosorption capacity.…”