In vitro evaluation of the response of Dermanyssus gallinae to products in aqueous suspension

Abstract

Dermanyssus gallinae are hematophagous mites that pose a serious sanitary problem in the Brazilian laying poultry industry. Its control is typically performed with acaricides, either in powder or liquid form.However, the intensive use of these products has led populations of this species to develop resistance. The aim of the present study is to evaluate the response of D. gallinae eggs and adults to products in aqueous suspension as per the commercial indication and registered at the Ministry of Agriculture, Livestock, and Supply. The study used four different acaricides (Product 1-Cypermethrin, Chlorpyrifos, and Piperonyl Butoxide; Product 2 -Alkyl-benzyl-dimethyl ammonium chloride, glutaraldehyde, deltamethrin; Product 3 -Dichlorvos; Product 4 -Fluralaner) tested in vitro using the contact method. Distilled water was used in the control group. There was difference between eggs and adults in each treatment (p < 0.05, except for control); products had higher efficacy against adult forms. This result might be directly related to frequent D. gallinae reinfestations in poultry houses.

In Vitro Acaricidal Activity of Silver Nanoparticles (AgNPs) against the Poultry Red Mite (Dermanyssus gallinae)

“…After 24 h, the mites were checked to assess how many were still alive and how many were dead (mortality) under the same binocular stereomicroscope used to identify them. According to the published methodology on D. gallinae bioassays [ 12 , 45 ], the mites were considered alive if they showed any form of repetitive movement, either spontaneously or in response to a gentle touch with a fine brush. For negative controls, natural mortality close to 10% was considered ideal.…”