In Vitro Evaluation of the Effects of Selected Plants on the Growth of the Mycelial Form of Histoplasma capsulatum Variety farciminosum in Ethiopia

Jaleta, Hawi; Ameni, Gobena; Arage, Mahlet; Giday, Mirutse; Girma, Musse; Sori, Teshale

doi:10.1016/j.jevs.2020.103139

Cited by 7 publications

(3 citation statements)

References 10 publications

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“…In another study, sensitivity and specificity of 83.3% and 80.64% were recorded in cart horses of Central Ethiopia [18]. A recent study of Histofarcin skin test in Bishoftu cart horses also reported 80% and 70% of sensitivity and specificity respectively [14], and again a year after 66.67% and 100% Sensitivity and specificity respectively were recorded at the same place [19]. However, in the current study, the diagnostic statistics were overestimated and this may be due to the smaller sample size of the field trial.…”

Section: Discussionmentioning

confidence: 88%

“…As a positive control ketoconazole (standard) was used and diluted at the concentrations of 0.8μg/mL, 0.4μg/mL,0.2 μg/mL, 0.1μg/mL, 0.05μg/mL, 0.025μg/mL, and 0.0125μg/mL. The dilutions corresponded within the range of MICs of Ketoconazole against isolates of HCF as reported by [14]. Cultures of HCF without any treatment served as negative controls.…”

Section: Preparation Of Croton Macrostachyus Extractmentioning

confidence: 99%

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Histofarcin Skin Test and an In-Vitro Antifungal Effects of Croton Macrostachyus against Mycelial Form of Histoplasma capsulatum Variety Farciminosum isolated from Horse in Adama City, Ethiopia

Tegegne,

Woldegiorgis

2023

Preprint

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Background: Epizootic Lymphangitis (EL) is a chronic debilitating disease of equines caused by Histoplasma capsulatum variety farciminosum (HCF). It is one of the major equine diseases with economics and equines welfare consequences. An experimental study design was conducted from Nov, 2021 to June, 2022 to assess the histofarcin skin test and evaluate the antifungal efficacy of Croton macrostachyus against the mycelium form of HCF. The histofarcin antigen used for histofarcin skin test was prepared from field isolates of HCF, identified from clinical cases of EL in cart horses from Adama City. Result: Then, histofarcin skin test was evaluated on 60 horses, 15 clinically infected cases, and 45 healthy horses. Each horse was injected with 0.1ml of the histofarcin skin test antigen intradermally in the middle of the neck. The thickness of the skin was measured before the injection and 24 hrs post injection using Bar Knight Mc Lintock(McLK)-P0005) caliper. The sensitivity and specificity of histofarcin skin test were 100% and 95% respectively. An in vitro growth inhibitory effect of Croton macrostachyus against the mycelia form of HCF was evaluated. Two-fold serial dilutions of the methanol extracts of the plant were prepared and assessed. A culture media with no antifungal agent and media containing ketoconazole served as a negative and positive control, respectively. Conclusion:The results showed that Croton macrostachyus have an inhibitory effect at concentrations ranging from 2.5mg/ml–10mg/ml. Thus, the minimum inhibitory concentration of the plant extract was found to be 2.5mg/ml. In conclusion, this study demonstrated that histofarcin skin test is a potential diagnostic tool for screening EL. Also, the methanol extracts of Croton macrostachyus showed good in-vitro growth inhibitory activity against the mycelial phase of HCF. Therefore, Validation of the performance of the histofarcin skin test assessment is necessary. Also, further investigation of the effects of C. macrostachyus on the yeast form of HCF is needed in addition to Phytochemial analysis (fractionation) and evaluation of the toxicity of the plant.

show abstract

Section: Discussionmentioning

confidence: 88%

Section: Preparation Of Croton Macrostachyus Extractmentioning

confidence: 99%

Histofarcin Skin Test and an In-Vitro Antifungal Effects of Croton Macrostachyus against Mycelial Form of Histoplasma capsulatum Variety Farciminosum isolated from Horse in Adama City, Ethiopia

Tegegne,

Woldegiorgis

2023

Preprint

View full text Add to dashboard Cite

show abstract

“…In another study, sensitivity and speci city of 83.3% and 80.64% were recorded in cart horses of Central Ethiopia [18]. A recent study of Histofarcin skin test in Bishoftu cart horses also reported 80% and 70% of sensitivity and speci city respectively [14] and again a year after 66.67% and 100% Sensitivity and speci city respectively were recorded at the same place [19]. However, in the current study, the diagnostic statistics were overestimated and this may be due to the smaller sample size of the eld trial.…”

Section: Discussionmentioning

confidence: 95%

Testing the skin with Histofarcin and examining the in-vitro antifungal properties of Croton Macrostachyus against the mycelial form of Histoplasma capsulatum Variety Farciminosum, isolated from a horse in Adama City, Ethiopia

Tegegne,

Woldegiorgis

2023

Preprint

View full text Add to dashboard Cite

Background: Epizootic Lymphangitis (EL) is a long-lasting, incapacitating condition affecting horses, which is brought about by Histoplasma capsulatum variety farciminosum (HCF). This ailment is a significant concern in the equine world, leading to both economic and welfare implications for horses. Method: An experimental study design was conducted from Nov, 2021 to June, 2022 to examine the histofarcin skin test and assess the antifungal effectiveness of Croton macrostachyus against HCF mycelium. The histofarcin antigen for the skin test was derived from HCF field isolates identified in clinical cases EL in cart horses from Adama City. Results: Histofarcin skin test was evaluated on 64 horses, 20 clinically infected cases, and 44 healthy horses. Each horse was injected with 0.1ml of the histofarcin skin test antigen intradermally in the middle of the neck. The thickness of the skin was measured before the injection and 24 hrs post injection using Bar Knight Mc Lintock(McLK)-P0005) caliper. The sensitivity and specificity of histofarcin skin test were 100% and 95% respectively. An in vitro growth inhibitory effect of Croton macrostachyus against the mycelia form of HCF was evaluated. Aculture media with no antifungal agent and media containing ketoconazole served as a negative and positive control, respectively. Conclusions: The result showed that Croton macrostachyus have an inhibitory effect at concentration range from2.5-10mg/ml. Thus, the minimium inhibitory concentration of the plant extrac was found to be 2.5mg/ml. In conclusion, this study demonstrated that histofarcin skin test is a potential diagnostic tool for screening EL. Also, the methanol extracts of Croton macrostachyus showed good in-vitro growth inhibitory activity against the mycelial phase of HCF. Therefore, Validation of the performance of the histofarcin skin test assessment is necessary. Also, further investigation of the effects of C. macrostachyus on the yeast form of HCF is needed in addition to Phytochemial analysis (fractionation) and evaluation of the toxicity of the plant.

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Medicinal properties and anti-inflammatory components of Phytolacca (Shanglu)

Bailly

2021

Digital Chinese Medicine

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In Vitro Evaluation of the Effects of Selected Plants on the Growth of the Mycelial Form of Histoplasma capsulatum Variety farciminosum in Ethiopia

Cited by 7 publications

References 10 publications

Histofarcin Skin Test and an In-Vitro Antifungal Effects of Croton Macrostachyus against Mycelial Form of Histoplasma capsulatum Variety Farciminosum isolated from Horse in Adama City, Ethiopia

Histofarcin Skin Test and an In-Vitro Antifungal Effects of Croton Macrostachyus against Mycelial Form of Histoplasma capsulatum Variety Farciminosum isolated from Horse in Adama City, Ethiopia

Testing the skin with Histofarcin and examining the in-vitro antifungal properties of Croton Macrostachyus against the mycelial form of Histoplasma capsulatum Variety Farciminosum, isolated from a horse in Adama City, Ethiopia

Medicinal properties and anti-inflammatory components of Phytolacca (Shanglu)

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