In vitro evaluation of the herbal cream formulation from methanolic leaf extracts of Tephrosia vogelii Hook.f for topical application

Abstract

Background Topical infectious are among of global challenges which propelled up with antimicrobial resistances. This scenario compels for drug discovery and development. Herbal formulation is one of important struggle in the drug development with the purpose of overcoming various diseases distressing health care settings. Thus, this study aimed to formulate and evaluate herbal cream from leaf methanolic extracts of Tephrosia vogelii Hook.f for topical therapies. Methods Herbal cream was prepared by homogeneously mixing up cream base and methanolic leaf extracts of Tephrosia vogelii. Physicochemical properties and in vitro antimicrobial activities of the cream were evaluated. The stability, colour change, washability and texture were parameters used evaluate physicochemical properties of cream. Disc diffusion method was employed to evaluate antimicrobial activities of the cream against Candida albicans (ATCC 90,028), Escherichia coli (ATCC29953) and Staphylococcus aureus (ATCC25923). Results Formulated herbal creams; CBTV1, CBTV2 and CBTV3 with methanolic extracts concentrations content of Tephrosia vogelii 0.05%, 0.10% and 0.25%, respectively, were prepared. All cream formulations were stable, washable by water, green in colour and soft. The CBTV3 product exhibited with high prominent antimicrobial activity compared to the rest, henceforth promisingly to be effective for topical skin therapy. Conclusion The herbal cream formulation of methanolic leaf extract of Tephrosia vogelii at 0.25% appeared to have more antimicrobial performance. Therefore, it is considered as minimal concentration for the herbal cream formulation in future for clinical trials as a potential antifungal and antibacterial agent product for treatment of skin infections. Graphical Abstract

The Formulation of Dermato-Cosmetic Products Using Sanguisorba minor Scop. Extract with Powerful Antioxidant Capacities

“…This cream had precautionary roles in UVB-induced skin damage such as MMP-2 expression (matrix metalloproteinase), MMP-9 expression (matrix metalloproteinase), IL-1β expression, and photoaging in mice. Mlozi et al [66] formulated herbal creams from methanolic leaf extracts of Tephrosia vogelii for their antifungal and antibacterial properties [86]. In another study carried out on topical formulations containing Rosa canina extract, it was found that the formulations had antioxidant properties due to the presence of quercetin and its derivative quercetin-3-O-glucuronide, which inhibit ROS overproduction and offer chemoprotection for mitochondrial function through antioxidative action.…”

“…In another study carried out on topical formulations containing Rosa canina extract, it was found that the formulations had antioxidant properties due to the presence of quercetin and its derivative quercetin-3-O-glucuronide, which inhibit ROS overproduction and offer chemoprotection for mitochondrial function through antioxidative action. These creams were highly useful due to their bioactive contents, which included flavonoids, terpenes, tannins, and terpenoids [66,85,86].…”

Development and Evaluation of Topical Formulation Containing Agrimonia pilosa Extract

“…To reduce unexpected side effects and ensure expected effects, formulation and stability evaluation should be conducted for all products, including pharmaceuticals and cosmetics, and expiration dates must be set [14][15][16]. Recently, this importance has been gradually recognized and evaluations on the stability of natural products have been reported [17][18][19]. However, most did not analyze the ingredients and evaluate the stability.…”