In Vitro Evaluation of the Cytotoxic Potential of Thiosemicarbazide Coordinating Compounds in Hepatocyte Cell Culture

Abstract

Cancer is a global medical problem and, despite research efforts in the field of tumor treatment, there is currently a shortage of specific anticancer drugs. Most anticancer drugs show significant side effects. The liver is the organ that has central functions in drug metabolism, being a major target of the harmful action of anticancer compounds. In this context, it is essential to evaluate the cytotoxic effects of potential anticancer substances. Therefore, hepatotoxicity and hepatocyte viability were determined in vitro to evaluate the action of seven new local thiosemicarbazide coordination compounds (CCT) on normal liver cells. Doxorubicin was used as a reference substance. The control group consisted of hepatocytes not exposed to CCT action. The cell viability of hepatocytes treated with CCT decreased significantly by 5%-12% compared to the control, but was statistically significantly higher by 5%-14% compared to doxorubicin, except after CMD-8 and CMT-67 influence, when it does not change. Thus, new local CCT had a selective effect on hepatocytes in vitro and were less hepatotoxic compared to doxorubicin, which may be the basis for further study of its potential in anticancer drugs.

Copper Coordination Compounds With Thiosemicarbazones: In Vitro Assessment of Their Potential in Inhibiting Glioma Viability and Proliferation

“…There is a direct correlation between the reduction of resazurin in the growth medium and the quantity/proliferation of living organisms, ranging from bacteria to mammalian cells 37,38 . The reliability of the method for assessing cytotoxicity on fibroblasts 39 , cancer cell lines 40 , as well as evaluating cell proliferation in lymphocytes 41 , hepatocyte cultures 34 , and primary neuronal cells 42 has been confirmed.…”

“…Coordination compounds derived from thiosemicarbazones can intervene through various mechanisms in metabolic processes [29][30][31] , which can impact cellular function and viability. The mentioned effects have been reported in both normal and cancer cells [32][33][34][35][36] .…”

The Cell Viability of Dental Materials by The Lactate Dehydrogenase Assay

“…Cell-derived cytotoxicity studies, animal experiments, rat studies and laboratory studies have become frequently used in laboratories due to their ease of application and suitability for data 20,21 . The method used to evaluate cell viability and choosed in this study is the determination of lactate dehydrogenase (LDH) activity released from damaged/dead cells into the medium 22 .…”

Influence of doxorubicin on the extracellular matrix of the liver of rats under conditions of chronic alcoholic hepatitis

“…An increase in the concentration of the heparin-heparan fraction of glycosaminoglycans in the liver when doxorubicin is administered can be considered as an adaptive response to doxorubicin-induced oxidative damage to hepatocytes (Pantea et al, 2023). An increased concentration of the heparin-heparan fraction of glycosaminoglycans can activate hepatocyte proliferation through the midkine-associated cascade, which acquires the ability to activate due to the reduction of AMPK expression by doxorubicin (Ou et al, 2020;Wang et al, 2023).…”