In vitro evaluation of the antimicrobial activity of antiseptics against clinical Acinetobacter baumannii strains isolated from combat wounds

Denysko, Tetyana Valeriyivna; Назарчук, Олександр; Gruzevskyi, Oleksandr; Bahniuk, Nataliia Ànatoliivna; Dmytriiev, Dmytro Valeriiovych; Chornopyschuk, Roman Mykolayovych; Bebyk, Vira Volodymyrivna

doi:10.3389/fmicb.2022.932467

Cited by 11 publications

(8 citation statements)

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“…This article is a continuation of a study, part of which was published earlier [11], and was devoted to the antimicrobial properties of antiseptics against MDR clinical strains of Gram-negative bacteria, isolated from combat wounds. Our study is a fragment of scientific project «Research of the biological properties of pathogens of HCAIs and the development of means of combating them».…”

Section: Materials and Methods Of The Researchmentioning

confidence: 99%

“…MBC was taken as the lowest concentration of the antiseptic, at which colony growth does not occur under the given conditions. Three replicates were performed for each isolate of E. coli and antimicrobial component [11].…”

Section: Mic (Minimum Inhibitory Concentration) and Mbc (Minimum Bact...mentioning

confidence: 99%

“…IAA allows evaluating and comparing the effectiveness of antiseptics against microorganisms, regardless of their working concentrations. The interpretation of the results is based on the fact that under natural conditions the activity of antiseptics is reduced by four times, so the antiseptic was considered active if the IAA was greater than four (IAA>4) [3,11,26].…”

Section: Bs Iaa (Bacteriostatic Index Of Antiseptic Activity) and Bc ...mentioning

confidence: 99%

“…The positive control with which the results were compared was the average value of optical density for each strain without the addition of an antiseptic (instead of it, saline was added to the suspension) [9,11,13].…”

Section: Susceptibility Assays On Biofilm Formationmentioning

confidence: 99%

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In vitro evaluation of antimicrobial and anti-biofilm properties of antiseptics against multidrug resistant clinical Escherichia coli strains, isolated from combat wounds

Nazarchuk,

Denysko,

Dmytriiev

et al. 2023

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The necessity for the investigation of novel approaches and strategies for the treatment of multidrug-resistant E. coli related infections becomes more and more essential. Purpose - to investigate and compare the level of antimicrobial and anti-biofilm activity of antiseptic preparations against MDR clinical isolates of E. coli. Materials and methods. In vitro effectiveness of modern antiseptics; octenidine 0.1% (OCT), polyhexanide 0.1% (PHMB), chlorhexidine 0.5% (CHG), miramistin 0.01% (MRM), decamethoxine 0.1% 0.02% (DCM), povidone-iodine 10% (PVP-I), was determined against forty-six polyresistant clinical strains of E. coli. MIC, MBC were found by standard methods, the value of which was interpreted as a bacteriostatic and bactericidal index of antiseptic activity (BS IAA and BC IAA). The effect of antiseptics on the immature biofilm was modelled using the Christensen test. Results. MIC and MBC values were the lowest in DCM and OCT. The highest values of the antiseptic activity index (IAA>4) were determined for the antiseptics PHMB 0.1%, OCT 0.1% and DCM 0.1%. It was found that the feasibility of using MRM at a concentration of 0.01% is questionable as the BS IAA is above the threshold value, while the BC IAA is not. The effectiveness of PVP-I 1% against MDR E. coli was found insufficient. Sub-bacteriostatic concentrations of DCM, CHG, and PHMB reliably inhibited the formation of E. coli biofilms within 24 hours. MRM and PVP-I in sub-bacteriostatic concentrations stimulated biofilm formation. Conclusions. Based on the analysis of all conducted studies, 0.1% and 0.02% DCM, 0.05% CHG, 0.1% OCT, 0.1% PHMB, 10% and 2% PVP-I are the most active against MDR clinical isolates of E. coli. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.

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Section: Materials and Methods Of The Researchmentioning

confidence: 99%

Section: Mic (Minimum Inhibitory Concentration) and Mbc (Minimum Bact...mentioning

confidence: 99%

Section: Bs Iaa (Bacteriostatic Index Of Antiseptic Activity) and Bc ...mentioning

confidence: 99%

Section: Susceptibility Assays On Biofilm Formationmentioning

confidence: 99%

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In vitro evaluation of antimicrobial and anti-biofilm properties of antiseptics against multidrug resistant clinical Escherichia coli strains, isolated from combat wounds

Nazarchuk,

Denysko,

Dmytriiev

et al. 2023

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“…In vitro studies have demonstrated efficacy of PHMB on a Gram-negative bacteria and Gram-positive bacteria, [43][44][45][46][47] fungi, [48][49][50] certain viruses, 51,52 and single-celled parasites (amoeba). 53,54 PHMB has also been shown to show antibiofilm activity on biofilms, 31,47,48,[55][56][57][58] including wound biofilms.…”

Section: Experimental Assessment Of Anti-biofilm Activitymentioning

confidence: 99%

Effectiveness of a polyhexamethylene biguanide-containing wound cleansing solution using experimental biofilm models

Rippon

Rogers

Westgate³

et al. 2023

J Wound Care

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Objective: Antiseptics are widely used in wound management to prevent or treat wound infections, and have been shown to have antibiofilm efficacy. The objective of this study was to assess the effectiveness of a polyhexamethylene biguanide (PHMB)-containing wound cleansing and irrigation solution on model biofilm of pathogens known to cause wound infections compared with a number of other antimicrobial wound cleansing and irrigation solutions. Method: Staphylococcus aureus and Pseudomonas aeruginosa single-species biofilms were cultured using microtitre plate and Centers for Disease Control and Prevention (CDC) biofilm reactor methods. Following a 24-hour incubation period, the biofilms were rinsed to remove planktonic microorganisms and then challenged with wound cleansing and irrigation solutions. Following incubation of the biofilms with a variety of concentrations of the test solutions (50%, 75% or 100%) for 20, 30, 40, 50 or 60 minutes, remaining viable organisms from the treated biofilms were quantified. Results: The six antimicrobial wound cleansing and irrigation solutions used were all effective in eradicating Staphylococcus aureus biofilm bacteria in both test models. However, the results were more variable for the more tolerant Pseudomonas aeruginosa biofilm. Only one of the six solutions (sea salt and oxychlorite/NaOCl-containing solution) was able to eradicate Pseudomonas aeruginosa biofilm using the microtitre plate assay. Of the six solutions, three (a solution containing PHMB and poloxamer 188 surfactant, a solution containing hypochlorous acid (HOCl) and a solution containing NaOCl/HOCl) showed increasing levels of eradication of Pseudomonas aeruginosa biofilm microorganisms with increasing concentration and exposure time. Using the CDC biofilm reactor model, all six cleansing and irrigation solutions, except for the solution containing HOCl, were able to eradicate Pseudomonas aeruginosa biofilms such that no viable microorganisms were recovered. Conclusion: This study demonstrated that a PHMB-containing wound cleansing and irrigation solution was as effective as other antimicrobial wound irrigation solutions for antibiofilm efficacy. Together with the low toxicity, good safety profile and absence of any reported acquisition of bacterial resistance to PHMB, the antibiofilm effectiveness data support the alignment of this cleansing and irrigation solution with antimicrobial stewardship (AMS) strategies.

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Polihexanide

Kampf

2024

Antiseptic Stewardship

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In vitro evaluation of the antimicrobial activity of antiseptics against clinical Acinetobacter baumannii strains isolated from combat wounds

Cited by 11 publications

References 57 publications

In vitro evaluation of antimicrobial and anti-biofilm properties of antiseptics against multidrug resistant clinical Escherichia coli strains, isolated from combat wounds

In vitro evaluation of antimicrobial and anti-biofilm properties of antiseptics against multidrug resistant clinical Escherichia coli strains, isolated from combat wounds

Effectiveness of a polyhexamethylene biguanide-containing wound cleansing solution using experimental biofilm models

Polihexanide

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