In vitro evaluation of total mixed ration supplemented with exogenous fibrolytic enzymes for crossbred cows

Abstract

Aim:The study was conducted to evaluate the levels of exogenous fibrolytic enzymes (EFE) on in vitro digestibilities of dry matter (DM) and organic matter (OM), total gas production (TGP), metabolizable energy (ME) content, and microbial biomass production (MBP).Materials and Methods:The total mixed ration (TMR) was prepared using 30% each of sorghum hay and groundnut straw and 40% compound concentrate mixture to meet nutritional requirement of cow (500 kg) producing 12 kg fat corrected milk. The EFE was incorporated at 0, 40, 60, 80, 100, 120, 140, 160, 180, 200, 220, 240, 260, 280, 300, 320, 340, 360, 380, and 400 mg/kg TMR. The TMR substrates with different levels of EFE were in vitro incubated to ascertain their effect on digestibility, gas production, and nutritive values.Results:The significantly (p<0.05) higher and optimum in vitro digestibilities of DM (63.03%) and OM (63.62%) as well as TGP (72.35 ml/500 mg TMR) were observed at supplementation of 240 mg EFE/kg TMR, while ME (7.16 MJ/kg DM) and MBP (97.63 mg/500 mg TMR) were also better.Conclusion:The incorporation of EFE at 240 mg/kg TMR resulted significantly (p<0.05) higher and optimum in vitro digestibilities of DM and OM. The TGP, ME, and MBP were also better. The levels of EFE 240 mg/kg TMR were found suitable for further in vivo study in crossbred cows.

Digestibility Value and Fermentation Level of Local Feed-Based ration for Sheep

“…Meanwhile, feedstuffs having lower crude fiber generally has higher feed intake because the cell wall is thin thus can be penetrated by digestive latex (Riswandi et al, 2015). Rumen microorganism can synthesize and secresize cellulose enzyme β 1-4 which roles on hydrolyzing plant cell wall thus high crude fiber feedstuffs can be degraded into high quality feedstuffs (Lunagariya et al, 2017). Microorganism of synthesis up to 30-40 g/kg of digestible organic matter or equal to 187.5-250 g protein of microorganism/kg of digestible organic matter (Mayulu, 2014).…”

“…A qualified feed is an important factor to fulfill livestock growth needs to achieve maximum beef production as well as to achieve a successful livestock enterprise (Yakin et al, 2012) so income of farmers increase. Ruminant is a livestock commodity that can utilize a low feedstuffs quality and high crude fiber and then digests into a qualified feeds (Lunagariya et al, 2017). feedstuffs used to formulate ration should contain adequate nutrient content (protein, carbohydrate, fat, mineral, vitamin and water) for sheep thus can produce optimum result.…”

Nutritive and Anti-Nutritive Evaluation of Kleinhovia hospita, Leucaena leucocephala and Gliricidia sepium with Respect to Their Effects on in Vitro Rumen Fermentation and Gas Production

“…In the tropical and sub-tropical developing countries of the world, there is a wide and distinct difference between obtainable and needed livestock feeds. Ruminants in these areas are frequently fed highly lignified roughages and crop residues, which are low in available energy, nitrogen and minerals (Lunagariya et al, 2017). A large proportion of these plants compose of structural carbohydrate shielded by lignin, which is not easily degraded by rumen microbes (Sallam et al, 2007).…”

Fermentation of rain tree (Samanea saman) seed meal using mixed microbes to improve its nutritional quality

“…The digestibility of dry and organic matter in this study is higher than those reported in previous studies. A study by Lunagariya et al (2017) on mixed diets enhanced with external additives showed that the fibrinolytic enzymes resulted in a dry matter digestibility of 63.03% and an organic matter digestibility of 63.62%. Aslamyah et al (2017) showed that seaweed fermentation using a combination of microorganisms produces dry matter and organic matter digestibility of 60.92 and 57.77%, respectively.…”