1995
DOI: 10.1006/viro.1995.1189
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In Vitro Evidence That the Coat Protein of Alfalfa Mosaic Virus Plays a Direct Role in the Regulation of Plus and Minus RNA Synthesis: Implications for the Life Cycle of Alfalfa Mosaic Virus

Abstract: The coat protein of alfalfa mosaic virus has both structural and regulating functions. The latter is evident from the fact that the genomic RNAs of the virus, although they are of messenger polarity, cannot start an infection cycle in the absence of cost protein. The reason could be that the coat protein is needed for viral RNA synthesis. Indeed, the coat protein has been found in tight association with the viral RNA polymerase (R. Quadt et al., 1991, Virology 182, 309-315). To investigate the role of the coat… Show more

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Cited by 51 publications
(53 citation statements)
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“…RdRp isolated from mock-infected P12 plants does not contain CP but specifically transcribed AMV plus-strand RNAs in vitro into minus-strand copies (de Graaff & Jaspars, 1995). Addition of CP to this assay mixture inhibited minus-strand RNA synthesis.…”
Section: Replication Of Viral Rna : Role Of Coding Sequencesmentioning
confidence: 94%
See 1 more Smart Citation
“…RdRp isolated from mock-infected P12 plants does not contain CP but specifically transcribed AMV plus-strand RNAs in vitro into minus-strand copies (de Graaff & Jaspars, 1995). Addition of CP to this assay mixture inhibited minus-strand RNA synthesis.…”
Section: Replication Of Viral Rna : Role Of Coding Sequencesmentioning
confidence: 94%
“…In the absence of CP, the RdRp extracted from mock-infected P12 plants did not recognize AMV minus-strand RNA 3 as a template, but after addition of CP to the assay mixture plus-strand RNA 4 was synthesized (de Graaff & Jaspars, 1995). Also, when CP was added to membrane-bound AMV replication complexes that contain an endogenous minus-strand template, an increase in the accumulation of plus-strand RNA was observed.…”
Section: Replication Of Viral Rna : Role Of Coding Sequencesmentioning
confidence: 97%
“…[α-$#P]UTP was incorporated during the synthesis of the minusstrand RNA in vitro. The products were treated with S1 nuclease and subsequently analysed by electrophoresis on agarose gels and autoradiography, all essentially as previously described (Houwing & Jaspars, 1986 ;de Graaff et al, 1995). Fig.…”
mentioning
confidence: 99%
“…In the case of alfalfa mosaic virus, the coat protein is necessary for viral RNA synthesis and initiation of the infection cycle (15). Indeed, the coat protein was found to be tightly associated with the viral RNA polymerase, which was purified by immunoaffinity chromatography with antibodies raised against the coat protein (30).…”
Section: Transactivation Properties Of the Apmov Major Coat Proteinmentioning
confidence: 99%
“…Synthetic transcripts or purified genomic RNAs become infectious and able to replicate only upon the addition of few coat protein molecules. This phenomenon is known as genome activation and indicates that coat proteins have also a func-tional role in viral replication (15,16).…”
Section: Introductionmentioning
confidence: 99%