In vitro evolution of a Friedel–Crafts deoxyribozyme

Mohan, Utpal; Burai, Ritwik; McNaughton, Brian R.

doi:10.1039/c3ob40080h

Cited by 22 publications

(16 citation statements)

References 18 publications

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“…McNaughton and co-workers reported the in vitro evolution of a DNA hybrid catalyst for Friedel-Crafts alkylation reactions [25]. Using a biotinylated indole, which was initially intended for use in a pull-down assay, in combination with 5 0 -linked acyl imidazole DNA library, catalytically active DNAs were selected by a gel-shift assay.…”

Section: Alternative Dna Architecturesmentioning

confidence: 99%

DNA-based hybrid catalysis

Rioz‐Martínez

Roelfes

2015

Current Opinion in Chemical Biology

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Section: Alternative Dna Architecturesmentioning

confidence: 99%

DNA-based hybrid catalysis

Rioz‐Martínez

Roelfes

2015

Current Opinion in Chemical Biology

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“…Scott Silverman likewise added functionality to DNA libraries, eventually reporting amide bond cleavage [ 40 , 41 ]. This last case is historically remarkable, as several groups in this field had previously sought after sequence-specific peptide-cleaving aptazymes [ 42 , 43 , 44 , 45 , 46 ].…”

Section: Non-natural Nucleotides In Functional Nucleic Acids (Dnamentioning

confidence: 99%

Artificially Expanded Genetic Information Systems for New Aptamer Technologies

Biondi

Benner

2018

Biomedicines

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Directed evolution was first applied to diverse libraries of DNA and RNA molecules a quarter century ago in the hope of gaining technology that would allow the creation of receptors, ligands, and catalysts on demand. Despite isolated successes, the outputs of this technology have been somewhat disappointing, perhaps because the four building blocks of standard DNA and RNA have too little functionality to have versatile binding properties, and offer too little information density to fold unambiguously. This review covers the recent literature that seeks to create an improved platform to support laboratory Darwinism, one based on an artificially expanded genetic information system (AEGIS) that adds independently replicating nucleotide “letters” to the evolving “alphabet”.

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“…Recently, the interest in DNA has focused on the catalysis of organic synthesis [8][9][10]. Till now, DNA catalysts have four types, namely, ssDNA [11], human telomeric G-quadruplex DNA [12], ctDNA [11] and oligonucleotide [13]. The application of DNA-based hybrid catalysts in asymmetric catalysis in the form of a copper(II)-catalysed Diels-Alder reaction was first reported by Feringa and Roelfes in 2005 [14].…”

Section: Introductionmentioning

confidence: 99%

Catalysis of Henry Reaction by Plasmid DNA

Di¹,

Zhang²,

Y³

et al. 2018

Preprint

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This work demonstrates that a plasmid can function as an efficient biocatalyst in mediating the nitroaldol (Henry) reaction at high temperatures in ultrapure water. Compared with ssDNA, the plasmid displayed a remarkable catalytic efficiency (97% yield within 20 min). Aromatic aldehydes which bear strong or moderate electron-withdrawing groups reacted satisfactorily with nitromethane, which afforded the corresponding β-nitroalcohols in good yields within 2 h (up to 98%). By contrast, aliphatic and aromatic aldehydes with electron-donating groups did not react. Moreover, the yield significantly dropped when the size of the carbanion increased, and the yield was only 10% when nitropropane was used as the donor.

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In vitro evolution of a Friedel–Crafts deoxyribozyme

Cited by 22 publications

References 18 publications

DNA-based hybrid catalysis

DNA-based hybrid catalysis

Artificially Expanded Genetic Information Systems for New Aptamer Technologies

Catalysis of Henry Reaction by Plasmid DNA

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