In Vitro Experiments of Microwave Ablation in Liver Cancer Cells (Effects of Microwave Power and Heating Time)

Manop, Purachet; Keangin, P.; Nasongkla, Norased; Eawsakul, Komgrit

doi:10.1109/iciea49774.2020.9102010

Cited by 4 publications

(1 citation statement)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, targeted proteins relating to breast cancer such as ERα, PR, EGFR, HER2, Pgp, and NF-κB were analyzed for impact after being treated with MUC-30 by the estimation of IC 50 values calculated by AutoDock [17]. Results were compared to IC 50 obtained by MTT assay [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of MUC-30-Loaded Polymeric Micelles against MCF-7 Cell Lines Using Molecular Docking Methods and In Vitro Study

Nasongkla

Tuchinda

Munyoo

et al. 2021

Evidence-Based Complementary and Alternative Medicine

Self Cite

View full text Add to dashboard Cite

MUC-30 is a hydrophobic compound which is active against the MCF-7 cancer cell line. In this study, MUC-30 was loaded in polymeric micelles to improve the water solubility and release rate. For prolonged MUC-30 release, MUC-30 was encapsulated in polymeric micelles using PEG-b-PLA and PEG-b-PCL as materials. Micelles prepared with 1 : 9 w per w ratios by film hydration achieved the highest entrapment efficiency (EE%). The EE% of MUC-30-loaded PEG-b-PCL micelles was approximately 30% greater than that of PEG-b-PLA micelles, due to the different H-bond formations between MUC-30 and the polymer membrane (PCL, 4; PLA, 3). The cytotoxic activity of MUC-30 against EGFR theoretically presented 399.31 nM (IC50 = 282.26 ng/mL) by molecular docking. In vitro cytotoxic activity of MUC-30 was confirmed by MTT assay. MUC-30 (IC50 = 11 ± 0.39 ng/mL) showed three-fold higher activity over MUC-30-loaded PEG-b-PLA micelles (IC50 = 37 ± 1.18 ng/mL) and two-fold higher over PEG-b-PCL micelles (IC50 = 75 ± 3.97 ng/mL). This was due to the higher release rate of MUC-30 from PEG-b-PLA micelles compared to PEG-b-PCL micelles. Therefore, MUC-30-loaded PEG-b-PLA micelles could be a promising candidate for breast cancer chemotherapy.

show abstract