In vitro fermentation and production of methane and carbon dioxide from rations containing Moringa oleifera leave silage as a replacement of soybean meal: in vitro assessment

Morsy, Tarek A.; Gouda, Gouda A.; Kholif, Ahmed E.

doi:10.1007/s11356-022-20622-2

Cited by 24 publications

(14 citation statements)

References 43 publications

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“…This can be attributed to the content of easily fermentable carbohydrates in each diet, as they provide energy to the ruminal microbiota for their metabolic activities during the first hours of fermentation [42], as was observed after 24 h in the AH10 and AH25 diets, which presented a high total gas production. In contrast, the AH100 diet showed fewer fast-fermenting carbohydrates and higher total gas production up to 48 h, indicating that rumen microbes took time to adapt to the diet and had less energy available for their offspring activities compared to the other diets [43].…”

Section: In Vitro Ruminal Total Gas Productionmentioning

confidence: 86%

Effect of Dietary Guanidinoacetic Acid Levels on the Mitigation of Greenhouse Gas Production and the Rumen Fermentation Profile of Alfalfa-Based Diets

Vazquez-Mendoza

Andrade-Yucailla

Elghandour

et al. 2023

Animals

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The objective of this study was to evaluate the effect of different percentages of alfalfa (Medicago sativa L.) hay (AH) and doses of guanidinoacetic acid (GAA) in the diet on the mitigation of greenhouse gas production, the in vitro rumen fermentation profile and methane (CH4) conversion efficiency. AH percentages were defined for the diets of beef and dairy cattle, as well as under grazing conditions (10 (AH10), 25 (AH25) and 100% (AH100)), while the GAA doses were 0 (control), 0.0005, 0.0010, 0.0015, 0.0020, 0.0025 and 0.0030 g g−1 DM diet. With an increased dose of GAA, the total gas production (GP) and methane (CH4) increased (p = 0.0439) in the AH10 diet, while in AH25 diet, no effect was observed (p = 0.1311), and in AH100, GP and CH4 levels decreased (p = 0.0113). In addition, the increase in GAA decreased (p = 0.0042) the proportion of CH4 in the AH25 diet, with no influence (p = 0.1050) on CH4 in the AH10 and AH100 diet groups. Carbon monoxide production decreased (p = 0.0227) in the AH100 diet with most GAA doses, and the other diets did not show an effect (p = 0.0617) on carbon monoxide, while the production of hydrogen sulfide decreased (p = 0.0441) in the AH10 and AH100 diets with the addition of GAA, with no effect observed in association with the AH25 diet (p = 0.3162). The pH level increased (p < 0.0001) and dry matter degradation (DMD) decreased (p < 0.0001) when AH was increased from 10 to 25%, while 25 to 100% AH contents had the opposite effect. In addition, with an increased GAA dose, only the pH in the AH100 diet increased (p = 0.0142 and p = 0.0023) the DMD in the AH10 diet group. Similarly, GAA influenced (p = 0.0002) SCFA, ME and CH4 conversion efficiency but only in the AH10 diet group. In this diet group, it was observed that with an increased dose of GAA, SCFA and ME increased (p = 0.0002), while CH4 per unit of OM decreased (p = 0.0002) only with doses of 0.0010, 0.0015 and 0.0020 g, with no effect on CH4 per unit of SCFA and ME (p = 0.1790 and p = 0.1343). In conclusion, the positive effects of GAA depend on the percentage of AH, and diets with 25 and 100% AH showed very little improvement with the addition of GAA, while the diet with 10% AH presented the best results.

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Section: In Vitro Ruminal Total Gas Productionmentioning

confidence: 86%

Effect of Dietary Guanidinoacetic Acid Levels on the Mitigation of Greenhouse Gas Production and the Rumen Fermentation Profile of Alfalfa-Based Diets

Vazquez-Mendoza

Andrade-Yucailla

Elghandour

et al. 2023

Animals

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“…This could also be linked to a reduction in the protozoan population when MGL was included in the ration. Morsy et al [52] hypothesize that the decrease in total rumen protozoa counts seen in diets containing secondary plant compounds may help to explain the higher rumen bacterial counts. When protozoan populations decline, the rumen environment becomes more conducive to the growth and proliferation of bacteria.…”

Section: Discussionmentioning

confidence: 99%

Utilization of marigold leaves (Tagetes erecta L.) in rations and their effect on rumen enzyme activity, fermentation parameters, methane emission, and nutrient digestibility in vitro

Hanim,

Anam,

Yusiati

et al. 2024

J Adv Vet Anim Res

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Objective: This study evaluated the utilization of marigold leaves (MGLs) in rations and their impact on rumen enzyme activity, fermentation parameters, methane (CH4) emission, and nutrient digestibility in vitro. Materials and Methods: The experimental diets comprised different proportions of MGL incorporated into the dry matter (DM) rations. Experimental design: The MGL treatments in diets include 0% (MGL-0), 7% (MGL-7), and 14% (MGL-14). Results: Results indicated that MGL-14 substantially raised (p < 0.05) the rumen parameters, including NH3–N and microbial protein, total volatile fatty acids, acetate (C2), propionate (C3), butyrate (C4), and the C2:C3 ratio. In contrast, the MGL-7 and MGL-14 groups experienced a noteworthy reduction (p < 0.05) in the total protozoa population. The MGL-7 and MGL-14 treatments also led to a substantial increase in the digestibility of DM, organic matter (OM), and crude fiber (CF) in the rumen. However, they also resulted in a decline (p < 0.05) in crude protein (CP) digestibility. The DM and OM total digestibilities were higher (p < 0.05) in the MGL-14 and MGL-7 groups. The utilization of MGL did not influence (p > 0.05) the ruminal enzyme activities (carboxymethyl cellulase, amylase, protease), cumulative gas production, kinetics, ruminal pH value, CH4 and CO2 production, total CF, and CP digestibility. Conclusion: The utilization of MGL until 14% DM in diets can enhance ruminal fermentation parameters and nutrient digestibility in vitro without negatively affecting gas production kinetics or ruminal enzyme activities. However, it did not have any impact on CH4 production.

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“…Increase in propionate production also represents a change in rumen fermentation to reduce methane emission (Polyorach et al 2014 ). Moringa leaves silage increased the total gas production, acetate, propionate while reduced the ruminal protozoa population and methane production (Morsy et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Impact of Moringa oleifera on rumen fermentation and methane emission under in vitro condition

et al. 2022

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Exploring innovative methods to provide essential nutrients and reducing ruminant greenhouse gas emission is crucial for animal production and diminishing global warming. This study was conducted to examine the efficacy of Moringa oleifera leaves (ML) in ruminants at 0%, 5%, 10%, 15%, 20%, 30% and 40% level in different roughage (R) and concentrate (C) (80R:20C, 70R:30C and 60R:40C) under in vitro conditions. Chemical composition of ML, concentrate mixture and berseem were estimated. Rumen fermentation parameters of male goat kids viz., total gas production, CH4, true dry matter digestibility (TDMD), organic matter digestibility (TOMD), partial fraction (PF), microbial biomass (MBP), ammonia (N), acetate, propionate, butyrate and acetate propionate ratio were observed under in vitro conditions. Results revealed that crude protein, organic matter and ethyl ether content were higher in ML as compared to concentrate mixture and berseem. Magnesium and iron content were also higher in ML as compared to concentrate and berseem. Total gas production, digestibility of DM and OM, MBP, acetate and propionate level were improved (P < 0.05) upto 10–20% replacement. In contrast, decreased in CH4 (%) and CH4 (mL/100 mg dDM) was noted with increased levels of ML incorporation. There was no change observed in ammonia, acetate: propionate ratios at all the three planes of nutrition. In this study, it is concluded that mixing Moringa oleifera leaves in feed can be used as protein supplement and reduce the methane emission without causing any effect on digestibility and rumen fermentation parameters. However, ML can be suggested for widespread practice to attain the sustainable animal production (10–20%) and to alleviate the global warming.

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In vitro fermentation and production of methane and carbon dioxide from rations containing Moringa oleifera leave silage as a replacement of soybean meal: in vitro assessment

Cited by 24 publications

References 43 publications

Effect of Dietary Guanidinoacetic Acid Levels on the Mitigation of Greenhouse Gas Production and the Rumen Fermentation Profile of Alfalfa-Based Diets

Effect of Dietary Guanidinoacetic Acid Levels on the Mitigation of Greenhouse Gas Production and the Rumen Fermentation Profile of Alfalfa-Based Diets

Utilization of marigold leaves (Tagetes erecta L.) in rations and their effect on rumen enzyme activity, fermentation parameters, methane emission, and nutrient digestibility in vitro

Impact of Moringa oleifera on rumen fermentation and methane emission under in vitro condition

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