In Vitro Fermentation of NUTRIOSE® FB06, a Wheat Dextrin Soluble Fibre, in a Continuous Culture Human Colonic Model System

Abstract

Wheat dextrin soluble fibre may have metabolic and health benefits, potentially acting via mechanisms governed by the selective modulation of the human gut microbiota. Our aim was to examine the impact of wheat dextrin on the composition and metabolic activity of the gut microbiota. We used a validated in vitro three-stage continuous culture human colonic model (gut model) system comprised of vessels simulating anatomical regions of the human colon. To mimic human ingestion, 7 g of wheat dextrin (NUTRIOSE® FB06) was administered to three gut models, twice daily at 10.00 and 15.00, for a total of 18 days. Samples were collected and analysed for microbial composition and organic acid concentrations by 16S rRNA-based fluorescence in situ hybridisation and gas chromatography approaches, respectively. Wheat dextrin mediated a significant increase in total bacteria in vessels simulating the transverse and distal colon, and a significant increase in key butyrate-producing bacteria Clostridium cluster XIVa and Roseburia genus in all vessels of the gut model. The production of principal short-chain fatty acids, acetate, propionate and butyrate, which have been purported to have protective, trophic and metabolic host benefits, were increased. Specifically, wheat dextrin fermentation had a significant butyrogenic effect in all vessels of the gut model and significantly increased production of acetate (vessels 2 and 3) and propionate (vessel 3), simulating the transverse and distal regions of the human colon, respectively. In conclusion, wheat dextrin NUTRIOSE® FB06 is selectively fermented in vitro by Clostridium cluster XIVa and Roseburia genus and beneficially alters the metabolic profile of the human gut microbiota.

Variable Responses to Carbon Utilization between Planktonic and Biofilm Cells of a Human Carrier Strain of Salmonella enterica Serovar Typhi

“…Pectin is a plant polysaccharide consisting of α-D-galacturonic acid and is a component of the primary cell walls of terrestrial plants [ 33 ]. Dextrins are produced in the human body as a result of enzymatic digestion of starch [ 34 ]. The KEGG pathway in the Supplementary S1 File showed that the enzymes produced for dextrin and pectin utilization were present in the CR0044 genome under the starch and sucrose metabolism ( S1 File ).…”

Abnormal fibre usage in UC in remission

“…The reduced capacity of the microbiota to ferment dietary carbohydrates could readily be examined using methodologies ex vivo by, for example, adapting faeces of patients with UC to models of gut microbiota 35. Examination of individual or collective bacteria populations has not been validated as a method of predicting fermentative capacity.…”

Fiber Supplements Derived From Sugarcane Stem, Wheat Dextrin and Psyllium Husk Have Different In Vitro Effects on the Human Gut Microbiota

“…In vivo studies conducted using inulin and various oligofructoses have shown an inhibition of animal and human pathogenic bacterial groups and increase in bifidobacteria and SCFAs (Saad et al, 2013 ). Several recent studies have also employed in vitro models to compare the effects of introducing pectin, inulin (Johnson et al, 2015 ; Chung et al, 2016 ), and wheat dextrin (Hobden et al, 2013 ) on the gut microbiota. These studies have demonstrated the potential of the fiber additions to enrich specific members of the genus Bacteroides and phylum Firmicutes (Hobden et al, 2013 ; Johnson et al, 2015 ; Chung et al, 2016 ).…”