In vitro fermentation properties of grape seed polysaccharides and the effect on regulating gut microbiota in mice

Deng, Huiling; Chen, Meiya; Fan, Cheng-Li; Ya, Chen; Wang, Fang; Zhou, Quan; Song, Can

doi:10.1007/s11694-023-02058-5

Cited by 4 publications

(2 citation statements)

References 40 publications

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“…The increase in Bacteroidota bacteria indicates that TPSs can be degraded and used by some Bacteroidota bacteria, promoting SCFA production in the intestines. This result is similar to that previously reported for Fuzhuan brick TPSs [24] and grapeseed polysaccharides [66].…”

Section: Changes In Gut Microbiome Composition In Fermentation Culturesupporting

confidence: 92%

In Vitro Characterization of Polysaccharides from Fresh Tea Leaves in Simulated Gastrointestinal Digestion and Gut Microbiome Fermentation

Zhou,

Gao,

Sun

et al. 2024

Foods

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Tea plants have a long cultivation history in the world, but there are few studies on polysaccharides from fresh tea leaves. In this study, tea polysaccharides (TPSs) were isolated from fresh tea leaves. Then, we investigated the characteristics of TPSs during in vitro simulated digestion and fermentation; moreover, the effects of TPSs on gut microbiota were explored. The results revealed that saliva did not significantly affect TPSs’ molecular weight, monosaccharide composition, and reducing sugar content, indicating that TPSs cannot be digested in the oral cavity. However, TPSs were partially decomposed in the gastrointestinal tract after gastric and intestinal digestion, resulting in the release of a small amount of free glucose monosaccharides. Our in vitro fermentation experiments demonstrated that TPSs are degraded by gut microbiota, leading to short-chain fatty acid (SCFA) production and pH reduction. Moreover, TPSs increased the abundance of Bacteroides, Lactobacillus, and Bifidobacterium but reduced that of Escherichia, Shigella, and Enterococcus, demonstrating that TPSs can regulate the gut microbiome. In conclusion, TPSs are partially decomposed by gut microbiota, resulting in the production of SCFAs and the regulation of gut microbiota composition and function. Therefore, TPSs may be used to develop a prebiotic supplement to regulate the gut microbiome and improve host health.

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Section: Changes In Gut Microbiome Composition In Fermentation Culturesupporting

confidence: 92%

In Vitro Characterization of Polysaccharides from Fresh Tea Leaves in Simulated Gastrointestinal Digestion and Gut Microbiome Fermentation

Zhou,

Gao,

Sun

et al. 2024

Foods

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“…In vitro simulated digestion and fecal fermentation experiments of PCY were carried out referenced to our team’s previous study [ 19 ]. All the saliva, intestinal fluid, gastric fluid and fermentation media used in the experiment were prepared according to previous studies [ 20 , 21 , 22 ].…”

Section: Methodsmentioning

confidence: 99%

Water-Insoluble Polysaccharide Extracted from Poria cocos Alleviates Antibiotic-Associated Diarrhea Based on Regulating the Gut Microbiota in Mice

Deng

Lei

et al. 2023

Foods

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Antibiotics are very effective in treating a variety of bacterial infections, while clinical overuse of antibiotics can lead to diseases such as antibiotic-associated diarrhea. Numerous studies have shown that natural polysaccharides can be used as prebiotics to alleviate antibiotic-associated diarrhea (AAD). Poria cocos is a medicinal and edible mushroom widely used for thousands of years in China, and our former study demonstrated that water-insoluble polysaccharide (PCY) has the potential prebiotic function. Therefore, we simulated the digestion and fermentation of PCY using feces from volunteers, and then administered it to C57BL/6 mice with AAD to study its effects on the gut microbiota and metabolites. The results indicated that PCY effectively alleviated the symptoms of AAD in mice, restored the intestinal barrier function, improved the content of short-chain fatty acids (SCFAs), decreased the level of inflammatory cytokines, and changed the structure of gut microbiota by increasing the relative abundance of norank_f__Muribaculaceae and unclassified_f__Lachnospiraceae, and decreasing that of Escherichia-Shigella, Staphylococcus and Acinetobacter. This study further demonstrated that PCY is an effective functional prebiotic for improving AAD disease, and provided a new avenue and insight for developing PCY as a functional food or prebiotic for alleviating gastrointestinal diseases.

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Polysaccharides of natural products alleviate antibiotic-associated diarrhea by regulating gut microbiota: a review

Lai,

Lan,

Qin

et al. 2024

Arch Microbiol

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In vitro fermentation properties of grape seed polysaccharides and the effect on regulating gut microbiota in mice

Cited by 4 publications

References 40 publications

In Vitro Characterization of Polysaccharides from Fresh Tea Leaves in Simulated Gastrointestinal Digestion and Gut Microbiome Fermentation

In Vitro Characterization of Polysaccharides from Fresh Tea Leaves in Simulated Gastrointestinal Digestion and Gut Microbiome Fermentation

Water-Insoluble Polysaccharide Extracted from Poria cocos Alleviates Antibiotic-Associated Diarrhea Based on Regulating the Gut Microbiota in Mice

Polysaccharides of natural products alleviate antibiotic-associated diarrhea by regulating gut microbiota: a review

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