In vitro fertilization and development of bovine oocytes matured with commercially available follicle stimulating hormone

Saeki, Kazuhiro; Hoshi, Masaki; Leibfried-Rutledge, M.L.; First, N. L.

doi:10.1016/s0093-691x(05)80002-0

Cited by 61 publications

(33 citation statements)

References 16 publications

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“…Groups of 10 CEOs were matured in vitro in 50-μl drops of IVMM covered with mineral oil (Nacalai Tesque, Kyoto, Japan). Matured oocytes were fertilized in vitro with frozen-thawed bull sperm as described [39], except with regard to the medium used [18]. In brief, frozen-thawed bull semen was layered onto a discontinuous Percoll gradient solution (45 % and 90 % [v/v]) and centrifuged at 700×g for 30 min.…”

Section: Chemicalsmentioning

confidence: 99%

Astaxanthin ameliorates heat stress-induced impairment of blastocyst development In Vitro: –astaxanthin colocalization with and action on mitochondria–

Kuroki

Ikeda

Okada

et al. 2013

J Assist Reprod Genet

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Section: Chemicalsmentioning

confidence: 99%

Astaxanthin ameliorates heat stress-induced impairment of blastocyst development In Vitro: –astaxanthin colocalization with and action on mitochondria–

Kuroki

Ikeda

Okada

et al. 2013

J Assist Reprod Genet

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“…The 7.5, 10, 12.5, 15, 17.5, 20, 22.5, 25, 27.5 and 30 (u/v) Percoll solutions (1.010, 1.013, 1.016, 1.019, 1.023, 1.026, 1.029, 1.032, 1.035 and 1.039 g/ml Percoll, respectively) were prepared by diluting 90% Percoll with mPBS just before use [11,12].…”

Section: Distribution Of Cocs and Oocytes By Sedimentation With Percollmentioning

confidence: 99%

“…Percoll, a colloidal polyvinylpyrrolidone-coated silica medium with low viscosity and nontoxicity to cells, is utilized for cell separation through sedimentation by standing or density gradient centrifugation [10]. Furthermore, the Percoll gradient method has been promoted for rapid and efficient isolation of bovine motile spermatozoa for in vitro fertilization [11,12]. However, it is not known whether COCs and oocytes can be distinguished by sedimentation with Percoll.…”

mentioning

confidence: 99%

A Simple Method for Selection of Cumulus-Oocyte Complexes from Bovine Ovaries by Sedimentation with Percoll

Yotsushima

Shimizu

Kon

et al. 2007

Journal of Reproduction and Development

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Abstract. Selection of bovine cumulus-oocyte complexes (COCs) for in vitro embryo production (IVP) is generally based on the morphological characteristics of the cumulus cells surrounding the oocytes and the ooplasm under microscopic observation. The purpose of this study was to examine a simple method for selection of COCs by sedimentation with Percoll solutions. COCs were aspirated from ovaries derived from a local slaughterhouse, and the COCs were classified by the morphology of their cumulus cell layers, as follows: Class A, compact and thick; Class B, compact but thin; Class C, partially denuded and thin; and Class D, denuded. Percoll solutions were prepared by diluting Percoll to 7.5, 10, 12.5, 15, 17.5, 20, 22.5, 25, 27.5 and 30% solutions, respectively. COCs were placed on the surface of the Percoll solution for 3 min, and the precipitated COCs were transferred to stepwise high density solution. The percentage of Percoll solution just before buoyancy was considered to the specific sedimentary value of the COC and oocyte. The mean sedimentary value of Class A COCs was higher than those of the other classes (P<0.01). The mean sedimentary values of denuded oocytes from Classes A and B were higher than those from Classes C and D (P<0.01). Our results show that sedimentation of COCs and denuded oocytes was generally related to the morphological quality of the COCs, although the sedimentary values ranged widely for one class of COCs and oocytes. The Percoll method can be used for simple selection of COCs. Key words: Bovine, Cumulus-oocyte complex, Percoll, Sedimentation (J. Reprod. Dev. 53: [971][972][973][974][975][976] 2007) n vitro maturation, fertilization and culture technologies for bovine oocytes are beneficial for increasing the number of embryos transferable to recipient cows. Various factors, including oocyte quality, culture media, protein source, growth factors and oxygen tension for the in vitro embryo production system (IVP), affect preimplantation embryo development in vitro [1][2][3]. Oocyte quality is an important factor affecting the success of an IVP system. Selection of oocytes for in vitro maturation is generally based on the quality of the cytoplasm and the characteristics of the cumulus cell investment around the oocyte [4,5]. These morphological criteria are routinely used to select the cumulus-oocyte complexes (COCs) with the most competent oocytes for embryo development. However, subjective evaluation of oocyte quality by morphological criteria under microscopic observation is thought to differ among evaluators. Recently, other means of characterizing bovine oocytes have been examined, such as evaluating the corona radiata density of cumulus-corona-oocyte complexes [6], distribution of cortical granules in oocytes [7] and expression of the LH receptor in

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“…Bovine 1-cell embryos used for microinjection were prepared by in vitro oocyte maturation and fertilization procedures as described previously [18]. Briefly, bovine cumulus-oocyte complexes (COCs) collected from ovaries obtained at local slaughterhouses were cultured for 21 h in 25 mM HEPES-buffered TCM-199 (Gibco Laboratories, Grand Island, NY, USA) supplemented with 0.3% (w/v) polyvinylpyrrolidone (PVP; M.W.…”

Section: In Vitro Maturation and Fertilization Of Bovine Oocytesmentioning

confidence: 99%

“…40,000, Sigma), 0.5 mM Na-pyruvate (Nacalai tesque, Kyoto, Japan), 1% antibiotic-antimycotic solution (Gibco), 0.02 AU/ml follicle stimulating hormone (Denka Seiyaku, Tokyo, Japan) and 1 µg/ml estradiol-17β (Sigma) at 39 C under 5% CO 2 in air with high humidity. For in vitro fertilization, frozen-thawed spermatozoa were washed with Percoll solution [18]. The spermatozoa were then suspended with defined medium (DM, [19]) modified by excluding glucose (m-DM).…”

Section: In Vitro Maturation and Fertilization Of Bovine Oocytesmentioning

confidence: 99%

Production of Bovine Transgenic Conceptus; Possible Selection of Transgenic Embryos by Polymerase Chain Reaction.

Hoshi

Saeki

Nagao

et al. 1999

Journal of Reproduction and Development

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Abstract. This study was conducted to examine the possibility of selection of transgenic bovine embryos by use of polymerase chain reaction (PCR) following microinjection of exogenous DNA before embryos were transferred to recipient cattle. DNA of bovine α-lactalbumin (α-LA) gene was microinjected into pronuclei of bovine embryos derived from in vitro maturation and fertilization. They were then cultured for 7 to 8 days to the blastocyst stage. To examine persistence of injected DNA during embryo development, embryos were analyzed by PCR at 0, 1, 3, 5 and 7 days following DNA microinjection. To distinguish the injected α-LA from the endogenous gene, gene-gene junction regions of head-to-tail concatemers of the injected DNA, which were formed when linearized DNA were injected into cell nuclei, were amplified by PCR. Injected DNA persisted at high rates (72 to 80%) until 5 days following DNA injection, but the detection rate at the blastocyst stage (20%) was lower than earlier stage (P<0.05), indicating that injected DNA into bovine embryos drastically decreased at the blastocyst stage. To determine if the PCR signals reflect transgenic status, biopsy samples of DNA-injected blastocysts were analyzed. Total of 1,583 embryos were microinjected, and 124 (8.9%) developed to blastocysts. Twelve (11%) out of 109 samples biopsied from microinjected blastocysts were positive by PCR analysis. Eight PCR-positive embryos were transferred to 8 recipients. Also, 31 negative embryos were transferred to recipient heifers. Four out of 8 recipients that received PCR-positive embryos were pregnant and fetuses and placentae were recovered at 60 to 75 days of pregnancy. One of the samples, of which only a placenta was recovered, was found to be transgenic by both PCR analysis and Southern hybridization. The other samples had no injected DNA. On the other hand, 14 fetuses with placentae were recovered from recipients that received PCR-negative embryos. None of these samples were found to carry injected DNA. Our system could not detect single, or head-to-head-or tail-to-tail-joining transgenes; however, the results described here imply selective transfer of potential transgenic embryos to recipient heifers by the PCR selection.

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In vitro fertilization and development of bovine oocytes matured with commercially available follicle stimulating hormone

Cited by 61 publications

References 16 publications

Astaxanthin ameliorates heat stress-induced impairment of blastocyst development In Vitro: –astaxanthin colocalization with and action on mitochondria–

Astaxanthin ameliorates heat stress-induced impairment of blastocyst development In Vitro: –astaxanthin colocalization with and action on mitochondria–

A Simple Method for Selection of Cumulus-Oocyte Complexes from Bovine Ovaries by Sedimentation with Percoll

Production of Bovine Transgenic Conceptus; Possible Selection of Transgenic Embryos by Polymerase Chain Reaction.

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