In vitro fertilization in the rat: observations on living eggs

Gaddum-Rosse, Penelope; Blandau, Richard J.; Langley, L.; Battaglia, David E.

doi:10.1016/s0015-0282(16)48028-3

Cited by 27 publications

(7 citation statements)

References 16 publications

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“…This impairment was similar to that of control spermatozoa in the absence of glucose, indicating a requirement for glucose in binding to the zona pellucida in rats. The binding of spermatozoa to the zona pellucida in the present study resembled that reported by Gaddum-Rosse et al (1984). In contrast, Urner and Sakkas (1996) showed that glucose is not required for binding of murine spermatozoa to the zona pellucida, and this could reflect differences in incubation conditions or species differences.…”

Section: Discussionsupporting

confidence: 77%

Effect of ornidazole on fertility of male rats: inhibition of a glycolysis-related motility pattern and zona binding required for fertilization in vitro

Bone¹,

Jones²,

Kamp³

et al. 2000

Reproduction

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The effects of the male antifertility agent ornidazole on glycolysis as a prerequisite for fertilization were investigated in rats. Antifertility doses of ornidazole inhibited glycolysis within mature spermatozoa as determined from the lack of glucose utilization, reduced acidosis under anaerobic conditions and reduced glycolytic enzyme activity. As a consequence, cauda epididymidal spermatozoa from ornidazole-fed rats were unable to fertilize rat oocytes in vitro, with or without cumulus cells, which was not due to transfer of an inhibitor in epididymal fluid with the spermatozoa. Under IVF conditions, binding to the zona pellucida was reduced in spermatozoa from ornidazole-fed males and the spermatozoa did not undergo a change in swimming pattern, which was observed in controls. The block to fertilization could be explained by the disruption of glycolysis-dependent events, since reduced binding to the zona pellucida and a lack of kinematic changes were demonstrated by control spermatozoa in glucose-free media in the presence of respiratory substrates. The importance of glycolysis for binding to, and penetration of, the zona pellucida, and hyperactivation in rats is discussed in relation to the glycolytic production of ATP in the principal piece in which local deprivation of energy may explain the reduced force of spermatozoa from ornidazole-fed males.

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Section: Discussionsupporting

confidence: 77%

Effect of ornidazole on fertility of male rats: inhibition of a glycolysis-related motility pattern and zona binding required for fertilization in vitro

Bone¹,

Jones²,

Kamp³

et al. 2000

Reproduction

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“…Acrosome-reacted sperm that bind and penetrate the zona pellucida are ready to fuse with the egg. During sperm-egg fusion, several authors have reported in different species that sperm stop moving (7)(8)(9). However, the mechanism behind this behavior is not established.…”

Section: Discussionmentioning

confidence: 99%

“…For example, in this final journey, most of sperm migrate to the ovulated eggs after acrosomal exocytosis, and very little information is available one motility in those sperm. In addition, during sperm-egg fusion, it is well documented that sperm stop moving (9)(10)(11) which is often considered as indicative of an effective fusion between gametes. The cease in sperm motility is necessary to complete the attachment and fusion between sperm and eggs, which is a complex event mediated by several proteins identified using loss of function strategies (12,13).…”

Section: Introductionmentioning

confidence: 99%

Reorganization of the Flagellum Scaffolding Induces a Sperm Standstill During Fertilization

Jabloñski

Luque

Gómez-Elías

et al. 2023

Preprint

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Mammalian sperm delve into the female reproductive tract to fertilize the female gamete. Within the midpiece of the sperm flagellum lies a cortical network of actin that is arranged as a double helix sheltering the mitochondrial sheath. This work demonstrates that the actin network of the midpiece undergoes structural changes that result in motility cessation. This structural modification is accompanied by a decrease in diameter of the midpiece and is driven by intracellular calcium changes that occur concomitant with a reorganization of the actin helicoidal cortex. Although midpiece contraction may occur in a subset of cells that undergo acrosomal exocytosis, live-cell imaging during in vitro fertilization showed that the midpiece contraction is required for motility cessation during the fusion process. These findings provide the first evidence of the F-actin network's role in regulating sperm motility, adapting its function to meet specific cellular requirements during fertilization.

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“…Because immature female rats are difficult to mate with adult males [2], the application of assisted reproductive technologies such as in vitro fertilization [6] or intracytoplasmic sperm injection [4] is required to obtain fertilized eggs. Generally, it takes approximately 2 to 3 weeks until post-pubertal rats exhibit stable estrus cycles with a defined 4-day period.…”

Section: Introductionmentioning

confidence: 99%

Production of Transgenic Rats Using Young Sprague-Dawley Females Treated with PMSG and hCG.

et al. 2001

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Abstract:The aim of this study was to examine the effects of gonadotrophin treatments on estrus synchronization and superovulation in young Sprague-Dawley (SD) rats that had not yet exhibited defined estrus cycles (5 to 7 weeks old), and to produce transgenic rats using these females as embryo donors and recipients. In Experiment 1, female rats were injected with PMSG and hCG (12.5, 25, 50 and 100 IU/kg each) and were mated with stud males. The reproductive performance of young rats were highest when PMSG and hCG at doses of 25 IU/kg each were injected (delivery rate 87.5%, nursing rate 92.9%). In Experiment 2, female rats were injected with PMSG and hCG (100, 150 and 300 IU/kg each) to induce superovulation. More eggs were recovered from the rats injected with PMSG and hCG at 150 and 300 IU/kg than from those treated with 100 IU/kg (33.4 and 41.3 vs. 13.3 eggs per female, respectively; p<0.05). In Experiment 3, pronuclear-stage zygotes from 150 IU/kg PMSG/hCG-treated rats were used for microinjection of the fusion gene of bovine αS1-casein gene promoter and human growth hormone gene (2.8 kb), and the microinjected zygotes were transferred into the oviduct ampullae of the 25 IU/kg PMSG/hCG-treated rats. Seventeen transgenic rats were obtained from the 334 DNA-injected zygotes (5.1%). These results indicate that recipients and embryo donors for the production of transgenic rats can be prepared by the appropriate PMSG and hCG treatments of young SD rats, regardless of their estrus stages.

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In vitro fertilization in the rat: observations on living eggs

Cited by 27 publications

References 16 publications

Effect of ornidazole on fertility of male rats: inhibition of a glycolysis-related motility pattern and zona binding required for fertilization in vitro

Effect of ornidazole on fertility of male rats: inhibition of a glycolysis-related motility pattern and zona binding required for fertilization in vitro

Reorganization of the Flagellum Scaffolding Induces a Sperm Standstill During Fertilization

Production of Transgenic Rats Using Young Sprague-Dawley Females Treated with PMSG and hCG.

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