In vitro fertilization of pig and sheep oocytes matured in vivo and in vitro

Cheng, Weijia; Moor, R. M.; Polge, C.

doi:10.1016/0093-691x(86)90200-1

Cited by 162 publications

(65 citation statements)

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“…The present polyspermy frequencies were comparable to those in other in-vitro fertilization studies in cattle (Parrish et al, 1986) and are low in comparison with findings for the pig (Cheng et al, 1986), suggesting that the block against polyspermy, at the level of zona and/or oolemma, was more efficient in cattle. Polygyny, probably resulting from failure of the first or second polar body to extrude, or from parthenogenetic activation (Xu et al, 1986a), was observed, but at a low incidence during the whole period studied.…”

Section: Discussionsupporting

confidence: 64%

A detailed analysis of early events during in-vitro fertilization of bovine follicular oocytes

Xu¹,

Grève²

1988

Reproduction

117

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Section: Discussionsupporting

confidence: 64%

A detailed analysis of early events during in-vitro fertilization of bovine follicular oocytes

Xu¹,

Grève²

1988

Reproduction

117

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“…In porcine sperm, a relatively high level of calcium (4.7 mM) is required for the induction of capacitation in vitro and for successful fertilization [5]. It is likely that the procedures stimulating Ca2' entry into sperm improve the results of the attempts for fertilization of porcine eggs in vitro.…”

Section: Measurement Of Intracellular Free Caz'mentioning

confidence: 99%

Forskolin stimulates porcine sperm capacitation by increasing calcium uptake

et al. 1993

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Using the fluorescent calcium indicator fura-2, forskolin was found to dose-dependently cause an immediate increase in the concentration of intracellular free calcium of porcine cauda epididymal sperm. This stimulatory effect of forskolin is due to the enhancement of Ca*+ uptake by the verapamil-sensitive transporter on the sperm plasma membrane and results in the promotion of the sperm capacitation and subsequent acrosome reaction.

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“…However such an approach requires a reliable supply of development-ally competent porcine embryos. The first successful birth of a piglet derived by in vitro fertilization (IVF) of porcine oocytes flushed from the reproductive tract was reported almost 3 decades ago (Cheng et al, 1986), and there have since been numerous reports of live births in pigs by IVF (Grupen, 2014), but obtaining in vivo matured oocytes is costly and time consuming (Nakai et al, 2003). Moreover, unlike cattle, non-surgical ovum pick up is not practical in pigs due to their anatomy.…”

Section: Introductionmentioning

confidence: 99%

Non-invasive assessment of porcine oocyte quality by supravital staining of cumulus–oocyte complexes with lissamine green B

Dutta

Fischer

et al. 2015

Zygote

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SummaryWe evaluated the usefulness of lissamine green B (LB) staining of cumulus-oocyte complexes (COC) as a non-invasive method of predicting maturational and developmental competence of slaughterhousederived porcine oocytes cultured in vitro. Cumulus cells of freshly aspirated COCs were evaluated either morphologically on the basis of thickness of cumulus cell layers, or stained with LB, which penetrates only non-viable cells. The extent of cumulus cell staining was taken as an inverse indicator of membrane integrity. The two methods of COC grading were then examined as predictors of nuclear maturation and development after parthenogenetic activation. In both cases LB staining proved a more reliable indicator than morphological assessment (P < 0.05). The relationship between LB staining and cumulus cell apoptosis was also examined. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay for DNA fragmentation revealed that oocytes within COCs graded as low quality by either LB staining or visual morphology showed significantly greater DNA fragmentation (P < 0.05) than higher grades, and that LB and visual grading were of similar predictive value. Expression of the stress response gene TP53 showed significantly higher expression in COCs graded as low quality by LB staining. However expression of the apoptosis-associated genes BAK and CASP3 was not significantly different between high or low grade COCs, suggesting that mRNA expression of BAK and CASP3 is not a reliable method of detecting apoptosis in porcine COCs. Evaluation of cumulus cell membrane integrity by lissamine green B staining thus provides a useful new tool to gain information about the maturational and developmental competence of porcine oocytes.

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In vitro fertilization of pig and sheep oocytes matured in vivo and in vitro

Cited by 162 publications

References 0 publications

A detailed analysis of early events during in-vitro fertilization of bovine follicular oocytes

A detailed analysis of early events during in-vitro fertilization of bovine follicular oocytes

Forskolin stimulates porcine sperm capacitation by increasing calcium uptake

Non-invasive assessment of porcine oocyte quality by supravital staining of cumulus–oocyte complexes with lissamine green B

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