In-vitro fertilization of preovulatory human eggs

Lopata, Alexander; McMaster, R.; McBain, John; Johnston, W. I. H.

doi:10.1530/jrf.0.0520339

Cited by 57 publications

(19 citation statements)

References 4 publications

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“…While the fertile life of primate sperm is much longer than previously believed, approximating 3 days and extend-extended period was necessary for sperm capacitation. Estimates for macaque sperm capacitation are 3-4 h (Marston & Kelly, 1968), while similar values (3-7 h) have been reported for man (Soup art & Strong, 1974;Lopata, McMaster, McBain & Johnson, 1978). Recent work in our laboratory has demonstrated that the in vitro capacitation time for squirrel monkey sperm used for in vitro fertilization trials ranged from 2·9 to 5·0 h (T. J. Kuehl & W. R. Dukelow, unpublished observations).…”

Section: Resultssupporting

confidence: 52%

Characteristics of the menstrual cycle in nonhuman primates. IV. Timed mating in Macaca nemestrina

Blakley¹,

Beamer²,

Dukelow

1981

Lab Anim

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SummaryThe events of the perineal swelling cycle in Macaca nemestrina have been correlated with the optimal time for conception. The mean cycle length of the animals was 32·8 days with the follicular and luteal phases of the cycle 17·6-19·2 and 13·6-15·2 days respectively. The time of ovulation, relative to the tumescence peak, ranged from I to 13 days. The optimal time for mating, based on the ratio between day of breeding and cycle length was 0·46, a value comparable with similar calculations for other macaque species.

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Section: Resultssupporting

confidence: 52%

Characteristics of the menstrual cycle in nonhuman primates. IV. Timed mating in Macaca nemestrina

Blakley¹,

Beamer²,

Dukelow

1981

Lab Anim

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“…Evidence to date suggests that neither the oviduct, the cells and matrix of the cumulus oophorus, the zona pellucida and other products of ovulation nor the oocyte itself are in any way essential for the onset of a normal acrosome reaction in mammalian spermatozoa (Saling & Bedford, 1981 ;Bedford, 1983). However, organic factors such as serum albumin and other 'serum factors' are essential additives to chemically defined media that permit spermatozoa to undergo capacitation and fertilization in vitro (mouse : Hoppe & Whitten, 1974;rat: Niwa & Chang, 1975;Davis, 1976; guinea-pig: Yanagimachi, 1972; Hyne & Garbers, 1981; golden hamster: Lui, Cornett & Meizel, 1977; Mrsny, Waxman & Meizel, 1979; Bavister, 1981 ; rabbit : Oliphant, 1976; dog: Mahi& Yanagimachi, ,1978;man: Yanagimachi, Yanagimachi & Rogers, 1976;Lopata, McMaster, McBain & Johnston, 1978). It has long been proposed that capacitation involves endogenous cellular changes that stem primarily from modifications of the plasmalemma of the spermatozoon (Barros, 1974;0'Rand, 1979;Oliphant &Singhas, 1979;Bearer & Friend, 1982), the state of which in turn may regulate a series of biochemical events associated with Ca2 + influx (Hyne & Garbers, 1979a, b; Hyne, Higginson, Kohlman & Lopata, 1984).…”

Section: Introductionmentioning

confidence: 99%

Influence of 2-deoxy-D-glucose and energy substrates on guinea-pig sperm capacitation and acrosome reaction

Hyne¹,

Edwards²

1985

Reproduction

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When guinea-pig spermatozoa were suspended in a minimal culture medium (MCM-PL), 2-deoxy-D-glucose (100 microM) and 2-amino-2-deoxy-D-glucose (100 microM) were potent inhibitors of the acrosome reaction without affecting the sperm motility, whereas the N-acetyl derivative 2-acetamido-2-deoxy-D-glucose (2 mM) had no inhibitory effect. The addition of D-glucose (2 mM) partly inhibited the percentage acrosome reaction of spermatozoa suspended in Medium MCM-PL, but DL-alpha-glycerophosphate (2 mM) and myo-inositol (2 mM) had no effect. In addition, DL-alpha-glycerophosphate (2 mM) did not overcome the inhibitory effect of 2-deoxy-D-glucose on the sperm acrosome reaction. The inhibitory action of 2-deoxy-D-glucose (100 microM) on the sperm acrosome reaction assessed after a 3-h incubation was irreversible and was only completely effective if the sugar was added within 30 min of the start of incubation. When spermatozoa suspended in Medium MCM-PL were treated with 2-deoxy-D-glucose (100-200 microM) for an extended incubation up to 6 h, the inhibitory effect of 2-deoxy-D-glucose was partly overcome. Spermatozoa treated with 2-deoxy-D-glucose had significantly reduced concentrations of ATP after incubation for 2 h in Ca2+-free media, compared with the ATP concentrations of spermatozoa preincubated for 2 h in Ca2+-free media that supported acrosome reactions. The addition of Ca2+ (5 mM) caused a rapid decrease in ATP concentrations of spermatozoa suspended in Medium MCM-PL, while the addition of the monovalent ionophore monensin (50 microM) and Ca2+ stimulated sperm acrosome reactions as well as an additional decline in the sperm ATP concentrations. However, monensin (50 microM) in the absence of Ca2+ caused only a slight decline in the sperm ATP concentrations over the 15-min incubation period. The depletion of the sperm ATP concentrations by 2-deoxy-D-glucose may retard completion of the capacitation process and the resultant acrosome reaction.

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“…In the analysis of the data, the preincubation time was classified as 160 rnin or >60 min, but adding the preparation period (postliquefaction) to the cutoff point gave a total time period of 150 min. This total time period is similar to that of Lopata et al [2], who used washed spermatozoa preincubated for 1.5 h to examine in vitro fertilization of preovulatory oocytes. Three hours after insemination, fertilization was c o n f m e d by light microscopy of serial sections of the ova.…”

Section: Discussionmentioning

confidence: 89%

“…In the absence of distinct markers, capacitation can be measured only by ability of sperm to fertilize eggs in vitro. The minimum time required for ejaculated human spermatozoa to acquire fertilizing ability in vitro is unknown [2]. Human spermatozoa preincubated for 1.5 h were able to fertilize preovulatory eggs within 3 h of their insemination in vitro.…”

mentioning

confidence: 99%