In Vitro Fertilization: The Challenge of the Eighties

Johnston, Ian; Lopata, Alex; Speirs, Andrew L.; Hoult, Ian J.; Kellow, Geoff; Plessis, Yvonne du

doi:10.1016/s0015-0282(16)45910-8

Cited by 82 publications

(28 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is most likely because the natural cycle depends solely upon the development of a dominant follicle (DF). As a result, ovarian stimulation was developed to generate more follicles and therefore more oocytes, replacing the natural cycle IVF [4,5]. This increases the probability of a successful pregnancy.…”

Section: Introductionmentioning

confidence: 99%

Fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle in vitro fertilization

Son

Chung

Das

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

Purpose To analyze the fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle IVF in women with regular cycles. Methods Natural cycle IVF was performed in 28 patients who had normal ovaries, > 6 antral follicle counts and were less than 40 years old (n028 cycles). An hCG trigger of 10,000 IU was administered 36 h before oocyte collection when the diameter of the dominant follicle (DF) was over 12 mm. Oocytes were retrieved from DF as well as from the cohort of smaller follicles. Embryological aspects of the mature and immature oocytes retrieved from these cycles as well as the implantation and clinical pregnancy rates depending on the origin of the embryos transferred were evaluated. Result(s) Overall clinical pregnancy and implantation rates were 20.8 % and 6.7 %, respectively. There were no differences in in vitro maturation (IVM), fertilization and embryo development between immature oocytes retrieved with and without in vivo matured oocytes. However, the clinical and implantation rates in cycles with embryos produced from in vivo matured oocytes transferred were better than the cycles where only IVM embryos were transferred (30.8 %, 9.1 % vs. 9.1 %, 3.2 %). Conclusion(s) Although our results show that immature oocytes from natural cycle IVF can fertilize normally and can be used to increase the number of embryos available for transfer, the embryos derived from the immature oocytes in natural cycles IVF have a poorer reproductive potential.

show abstract

Section: Introductionmentioning

confidence: 99%

Fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle in vitro fertilization

Son

Chung

Das

et al. 2012

J Assist Reprod Genet

View full text Add to dashboard Cite

show abstract

“…The high failure rate of implantation after IVF could be related to chromosomal aberrations in embryos. Only 10% of embryos are capable of inducing normal pregnancy after embryo replacement (Rudak et al, 1984), while the remaining 90% die before or immediately after transfer (Johnson et al, 1981). One explanation for the implantation failure is that embryos may carry lethal chromosome abnormalities which do not allow development beyond the preimplantation stage.…”

Section: Introductionmentioning

confidence: 99%

Abnormal chromosomal arrangements in human oocytes

et al. 1990

View full text Add to dashboard Cite

Ninety-one human oocytes, lacking signs of fertilization 50 h after insemination in vitro, were investigated cytogenetically to assess the frequency and type of chromosomal abnormalities. Chromosome spreading permitted adequate karyotyping in 55 oocytes. Non-determined numerical aberrations occurred with the following frequencies: hypohaploidy, 10.9% (6/55), hyperhapJoidy, 14.5% (8/55) and hyperdiploidy, 3.6% (2/55). Total aneuploidy occurred with a frequency of 29.1% and was observed in oocytes from 30 patients. No correlation was found between specific chromosomal aberrations and type of infertility, stimulation treatment or gonadotrophin levels. On the other hand, the frequency of aneuploidy was significantly higher (P < 0.05) in patients > 35 years of age. Two chromosomal complements (3.6%) had structural rearrangements; one oocyte had both structural and numerical chromosomal abnormalities and the other had differently condensed regions on the long arms of three chromosomes from group C. The overall frequency of chromosomal aberrations was 32.7%. Only two samples contained an additional set of polar body chromosomes. Thirteen oocytes presented sperm chromosomes in an arrested stage of premature chromosome condensation of the G, phase and four oocytes showed asynchronous condensation of pronuclear chromosomes. Finally, it was concluded that the high proportion of chromosomal aberrations observed in human oocytes may contribute significantly to abnormal embryonic development in vitro.

show abstract

“…Der natürliche Zyklus wurde mit Hilfe medikamentöser Manipulationen durch den stimulierten Zyklus ersetzt, um die Eizellausbeute pro Follikelaspiration zu erhöhen. Hintergrund dieser Entwicklung ist die Tatsache, dass die Eizellzahl mit der Zahl der zur Verfügung stehenden Embryonen und den daraus resultierenden Schwangerschaftswahrscheinlichkeiten korreliert [3,4,5]. Die heute äußerst erfolgreich praktizierte ovarielle Stimulationsbehandlung, die im Laufe ihrer Geschichte durch zahlreiche medikamentöse Innovationen wie hochgereinigte urinäre Gonadotropine, rekombinante Gonadotropine oder GnRH-Agonisten und -Antagonisten charakterisiert ist, weist jedoch Nachteile auf, die die Motivation für die Suche nach alternativen Behandlungsmöglichkeiten liefern [6].…”

Section: Hintergrundunclassified