In vitro fertilization using frozen-thawed feline epididymal spermatozoa from corpus and cauda regions

Kunkitti, Panisara; Axnér, Eva; Bergqvist, Ann-Sofi

doi:10.1016/j.theriogenology.2016.04.085

Cited by 15 publications

(17 citation statements)

References 19 publications

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“…The rate of embryo development after IVF for hybrid embryos revealed in the present study was comparable with the rate of in vitro development for homologous domestic cat embryos reported herein and elsewhere (Kunkitti et al, ). This rate of embryo development after heterologous IVF reported herein was close to or even higher than that after insemination of domestic cat oocytes with frozen–thawed spermatozoa of the fishing cat (Thiangtum et al, ) or the flat‐headed cat (Thuwanut et al, ); the two species belonging to the same genus Prionailurus as the Far‐Eastern cat.…”

Section: Discussionsupporting

confidence: 92%

“…The concentration of spermatozoa estimated herein for Far-Eastern wildcat ejaculates was well in the range reported earlier for the domestic cat (Zambelli & Cunto, 2006), but the volume of ejaculate for the former was much smaller. Characteristics for ejaculates of the Far-Eastern wildcat presented in this study are comparable with those reported by Pavlova The common methods to test the viability of spermatozoa after freezing-thawing are fluorescent and light microscopy accompanied with staining; estimation of sperm concentration, the percentages of live and dead sperms, and sperm motility; and evaluation of sperm morphology (Amstislavsky et al, 2017;Ha et al, 2011 test for semen fertilizing capacity is IVF followed by IVC (Kunkitti, Axnér, Bergqvist, & Sjunnesson, 2016;Thiangtum et al, 2006).…”

Section: Discussionsupporting

confidence: 82%

“…The common methods to test the viability of spermatozoa after freezing–thawing are fluorescent and light microscopy accompanied with staining; estimation of sperm concentration, the percentages of live and dead sperms, and sperm motility; and evaluation of sperm morphology (Amstislavsky et al, ; Ha et al, ). The ultimate test for semen fertilizing capacity is IVF followed by IVC (Kunkitti, Axnér, Bergqvist, & Sjunnesson, ; Thiangtum et al, ).…”

Section: Discussionmentioning

confidence: 99%

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Sperm cryopreservation in the Far‐Eastern wildcat (Prionailurus bengalensis euptilurus)

Amstislavsky

Brusentsev

Kizilova

et al. 2018

Reprod Domestic Animals

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The Far-Eastern wildcat (Prionailurus bengalensis euptilurus) is a rare and poorly investigated nondomestic felid species. An attempt of freezing and cryopreserving Far-Eastern wildcat spermatozoa in CaniPlus Freeze (CPF) medium is reported. Sperm was collected by electroejaculation from five adult Far-Eastern wildcat captive-born males. Epididymal spermatozoa from five adult randomly bred domestic cat males were used as a reference. The viability of frozen-thawed spermatozoa evaluated by double staining with SYBR Green I and PI followed by the subsequent confocal laser scanning microscopy (CLSM) was 38.2% ± 3.0% for the domestic cat and 38.0% ± 10.2% for the Far-Eastern wildcat. The motility of frozen-thawed spermatozoa was 30.8% ± 9.8% for the domestic cat and 33.7% ± 15.1% for the Far-Eastern wildcat. Sperm morphology was assessed by light microscopy. The total percentage of normal spermatozoa after freezing and thawing was 51.9 ± 5.9 for the domestic cat and 55.0% ± 6.4% for the Far-Eastern wildcat. Defects of flagella were the most frequently observed abnormalities in both species (32.2% ± 4.8% and 30.8% ± 4.4% of all reported anomalies for the domestic cat and Far-Eastern wildcat, respectively). Domestic cat epididymal and Far-Eastern ejaculatory spermatozoa fertilized in vitro-matured oocytes of the domestic cat (30.0% ± 5.5% and 35.5% ± 15.0%, respectively). Taken together, these results suggest that the freezing of Far-Eastern wildcat spermatozoa with CPF medium is a suitable method for Felidae cryopreservation.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

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Sperm cryopreservation in the Far‐Eastern wildcat (Prionailurus bengalensis euptilurus)

Amstislavsky

Brusentsev

Kizilova

et al. 2018

Reprod Domestic Animals

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“…Обычно процедуры замораживания -оттаивания снижают жизнеспособность сперматозоидов эпидидимального семени домашних котов на 20-30 % (Luvoni, 2006;Kunkitti et al, 2016;Prochowska et al, 2016).…”

Section: Discussionunclassified

Cryopreservation of epididymal semen of domestic cat

Amstislavsky¹,

Brusentsev²,

Mokrousova³

et al. 2017

Vestn. VOGiS

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Domestic cat (Felis silvestris catus)is used as a model species for developing effective methods of wild felids' semen cryopreservation. The present study represents a comparison of domestic cat semen cryopreservation with two commercially available cryoprotectant agents (CPAs): CaniPlus Freeze (CPF) and SpermFreeze (SF). Semen was collected from the caudal epididymises of adult males and frozen with CPF and SF, correspondingly. The viability of frozen-thawed spermatozoa was evaluated by VitalScreen kit, staining with hematoxylin and eosin was performed for analysis of the spermatozoa morphology; both analyses were combined with the light microscopy. The viability rate of the frozenthawed semen cryopreserved with CPF and SF did not differ: 32.3 ± 4.4 % for CPF and 43.3 ± 4.0 % for SF. Total percentage of morphologically normal spermatozoa after freezing and thawing domestic cat semen was 26.0 ± 2.3 % for CPF and 23.9 ± 1.9 % for SF. In both cases, there were no differences from non-frozen semen, in the latter group the total percentage of spermatozoa with normal morphology was 29.0 ± 4.1 %. The most frequent anomalies were the anomalies of tail, and the rarest anomalies were head defects. The percentages of spermatozoa with anomalies of the head, mid piece, tail and combined did not differ in these three groups. Taken together these results suggest that both CPAs are suitable for the purpose of domestic cat semen freezing and cryopreservation, although CPF was designed for Canidae semen cryopreservation and SF was developed for human semen freezing and so far was used exclusively in reproductive medicine. It might be concluded that these two commercially available cryoprotectant media are applicable for the purposes of domestic cat breeds' semen cryopreservation.Key words: domestic cat; epididymal spermatozoa; cryopreservation.Домашний кот (Felis silvestris catus) используется в качестве мо-дельного объекта для разработки эффективных методов крио-консервирования семени исчезающих видов кошачьих. В иссле-довании представлено сравнение двух коммерчески доступных криопротекторных смесей CaniPlus Freeze (CPF) и SpermFreeze (SF), используемых для криоконсервации семени домашнего кота (Felis silvestris catus). Семя получали из каудальных эпидидимисов взрос-лых самцов и замораживали с помощью CPF либо SF. Жизнеспо-собность замороженно-оттаянных сперматозоидов оценивали методом VitalScreen; морфологический анализ семени проводили с использованием окраски гематоксилином и эозином; в обоих случаях окраску сочетали со световой микроскопией. Доля живых сперматозоидов после замораживания с применением CPF и SF достоверно не различалась: 32.3 ± 4.4 % для CPF и 43.3 ± 4.0 % для SF. Доля морфологически нормальных сперматозоидов после процедур замораживания-оттаивания семени домашнего кота составила 26.0 ± 2.3 % для CPF и 23.9 ± 1.9 % для SF. В обоих случаях не было достоверных отличий по этому показателю от интактного (не подвергавшегося замораживанию) семени, в этой группе доля сперматозоидов с нормальной морфологией составила...

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“…Routine castrations or neutering/spaying of pets are performed on the explicit wishes of the owners and according to recommendations of veterinarians due to various reasons that can include prevention of unwanted offspring as well as changes in temperament or behaviour connected to hormonal actions in an intact animal (1). Normal routine castrations are performed daily in most veterinary clinics, and also here the ovaries are discarded as waste and can be collected for IVP purposes (2). This relatively easy access to large quantities of ovaries without having to use research animals makes species like the cow and pig perfect candidates for IVP research, spanning from testing of in vitro media and methodological improvements to controlled trials of newly emerging risk substances like environmental pollutants found in humans and our environment (3,4).…”

Section: An Overview Of Proceduresmentioning

confidence: 99%

In vitro fertilisation in domestic mammals—a brief overview

Sjunnesson

2019

Upsala Journal of Medical Sciences

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Many factors influence the final oocyte maturation, fertilisation, and early embryo development, and there are both similarities and differences between species. When comparing the advancement of assisted reproductive technologies (ARTs), the development in the bovine species is not far behind the medical front, with around one million in vitro-produced bovine embryos each year. This rate of progress is not seen in the other domestic species. This review aims to give an overview of the development and specific difficulties of in vitro embryo production in various domestic animal species, with the main focus on cows, pigs, and cats. In production animals, the aim of ARTs is commonly to increase the genetic progress, not to treat reproductive failure. The ARTs are also used for preservation of genetic diversity for the future. However, specifically for oocyte maturation, fertilisation, and early embryonic development, domestic mammals such as the cow and pig can be used as models for humans. This is particularly attractive from an animal welfare point of view since bovine and porcine oocytes are available in large numbers from discarded slaughterhouse material, thereby decreasing the need for research animals. Both for researchers on the animal and human medical fronts, we aim for the development of in vitro production systems that will produce embryos and offspring that are no different from those conceived and developed in vivo. Species-comparative research and development can provide us with crucial knowledge to achieve this aim and hopefully help us avoid unnecessary problems in the future. ARTICLE HISTORY

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In vitro fertilization using frozen-thawed feline epididymal spermatozoa from corpus and cauda regions

Cited by 15 publications

References 19 publications

Sperm cryopreservation in the Far‐Eastern wildcat (Prionailurus bengalensis euptilurus)

Sperm cryopreservation in the Far‐Eastern wildcat (Prionailurus bengalensis euptilurus)

Cryopreservation of epididymal semen of domestic cat

In vitro fertilisation in domestic mammals—a brief overview

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