In vitro flowering of Lilium rubellum Baker

Ishimori, Takateru; Niimi, Yoshiji; Han, Dongsheng

doi:10.1016/j.scienta.2008.11.017

Cited by 6 publications

(4 citation statements)

References 8 publications

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“…Early flowering and fruiting are possibly due to the presence of plant growth regulators in the medium. The medium containing growth regulators, especially cytokinin for a long time, the juvenile phase of the plant may be reduced (Te-Chato and Lim, 2004;Ziv and Naor, 2006;Ishimori et al, 2009;Jana and Shekhawat, 2011). This allows the plant to flower in a shorter time in comparison with seedlings and conventionally propagated trees.…”

Section: Hardening Of the Plantletsmentioning

confidence: 99%

In vitro propagation of traditional medicinal and dye yielding plant Morinda coreia Buch.–Ham

Shekhawat¹,

Kannan²,

Manokari³

2015

South African Journal of Botany

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An efficient micropropagation protocol has been developed successfully for Morinda coreia Buch.-Ham. by culturing nodal segments. The explants were washed, sterilized with HgCl 2 and inoculated on semi-solid Murashige and Skoog (MS) medium containing various concentrations and combinations of plant growth regulators (PGRs). Shoot bud initiation was observed after one week and 8.6 ± 0.32 shoots (per explant) harvested after five weeks on MS medium with 4.0 mg l −1 concentration of 6-benzylaminopurine (BAP). The regenerated shoots were further multiplied on semi-solid MS medium augmented with 2.0 mg l − 1 BAP + 1.0 mg l − 1 Kinetin (Kin). Maximum 24.5 ± 0.34 shoots per explant was obtained after five weeks on this media combination. The long (4-5 cm) and healthy shoots were rooted in vitro with 100% success rate on half strength MS medium + 1.0 mg l − 1 indole-3 butyric acid (IBA). Rooting and acclimatization were achieved simultaneously by ex vitro rooting method using 200 mg l − 1 IBA for 5 min with very good success rate (28.67 ± 05.51 roots per shoot with 100% response). The rooted shoots were transferred to the greenhouse for acclimatization for 4-5 weeks. The hardened plantlets were finally shifted to the field for further growth in the natural conditions after another five weeks. This is the first report on micropropagation of M. coreia, which can be successfully used for the large-scale multiplication and conservation of germplasm of this important medicinal plant.

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Section: Hardening Of the Plantletsmentioning

confidence: 99%

In vitro propagation of traditional medicinal and dye yielding plant Morinda coreia Buch.–Ham

Shekhawat¹,

Kannan²,

Manokari³

2015

South African Journal of Botany

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“…Different plant species require different induction media to induce in vitro flowers (Ziv and Naor 2006). Due to the genotypic diversity of different cultivars, the percentage of in vitro flowering differs from one cultivar to another (Douglas et al 1989, Wang et al 2002, Ishimori et al 2009. Wang et al (2009) reported that the total time from an original culture and subculture time before flower induction were two very important factors for in vitro flower induction.…”

Section: Discussionmentioning

confidence: 99%

“…Tiny (Duan and Yazawa 1994), Dendrobium cv. Chao Praya Smile (Hee et al 2007), Lilium rubellum (Ishimori et al 2009), Cymbidium niveo-marginatum (Kostenyuk et al 1999), Withania somnifera (Saritha and Naidu 2007), Dendrobium cv. Madame Thong-In (Sim et al2007), Dendrobium candidum (Wang et al 1997), Phalaenopsis (Duan and Yazawa 1995), Rosa cultivars (Wang et al 2002;Vu et al 2006), and Solanum nigrum (Sridhar and Naidu 2011).…”

Section: Discussionmentioning

confidence: 99%

In vitro flowering red miniature rose

Zeng¹,

Luo²,

Zhang³

et al. 2013

Biologia plant.

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Using aseptic plantlets obtained from stem node explants of hybrid red miniature rose (Rosa hybrida cv. Fairy Dance), the effects of shoot physiological status, medium ingredients, and culture thermoperiod on in vitro flowering were evaluated. Shoot height, subculture media for shoot multiplication, sucrose concentration, plant growth regulators (PGRs), mineral substances in media, and thermoperiod had a significant effect on the percentage of in vitro flowering. Shoots 3 ± 0.2 or 2 ± 0.2 cm in height cultured on Murashige and Skoog (MS) medium containing 2.0 mg dm -3 6-benzyladenine (BA), 0.2 mg dm -3 α-naphthaleneacetic acid (NAA), and 20 g dm -3 sucrose were more suitable for in vitro flowering than shoots 4 ± 0.2, or 5 ± 0.2 cm in height. The most suitable sucrose concentration for in vitro flowering was 50 g dm -3 and the most suitable PGRs were a combination of 3.0 mg dm -3 BA and 0.1 mg dm -3 NAA. Increasing the potassium nitrate to ammonium nitrate ratio or increasing the phosphate concentration in MS medium had a positive effect on in vitro flowering. The percentage of in vitro flowering was significantly higher at day/night temperature of 28/20 °C than at other constant temperatures. The percentage of in vitro flowering shoots reached 68.33 % despite the occurrence of abnormal flowers and some unique developmental patterns. It makes miniature rose a potentially new in vitro experimental platform for research on the molecular mechanisms of flowering ornamental plants.

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“…Orchids belongs to the family Orchidaceae, and they are perennial herbs having 25000 species spreading over the 730 genera widely cultivated over the world [1][2][3][4]. Some of the species are spreading over the plant called epiphytes some are growing in ground called terrestrial, some are growing in rocks known as lithophytes, some are growing on decaying matter known as saprophytes [5][6][7][8]. Some orchids grow in semi-aquatic…”

Section: Introductionmentioning

confidence: 99%

Orchids: A Wonderful Ornamental Plant

Husain,

Eraqui

2023

AJRCS

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The most exquisite flowers and long-lasting qualities of ORCHIDS make them among God's creations regarded as the most beautiful in the entire world. They are perennial herbs that are members of the Orchidaceae family. In addition to hybrids regularly created, there are reported to be nearly 25000 orchid species spread across 730 genera. Epiphytes include orchids, which are plants that grow on trees. Terrestrial refers to those that grow on the ground. Lithophytes are those that grow on rocks, while saprophytes are some relatively unknown ones that grow on decomposing matter or rotting logs. A few orchid species can grow partially submerged in water, with only the inflorescence occasionally reaching the surface.

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In vitro flowering of Lilium rubellum Baker

Cited by 6 publications

References 8 publications

In vitro propagation of traditional medicinal and dye yielding plant Morinda coreia Buch.–Ham

In vitro propagation of traditional medicinal and dye yielding plant Morinda coreia Buch.–Ham

In vitro flowering red miniature rose

Orchids: A Wonderful Ornamental Plant

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