In vitro Formation of Deoxycholic and Lithocholic Acid by Human Intestinal Microorganisms.

Norman, Arne; Shorb, Mary S.

doi:10.3181/00379727-110-27577

Cited by 56 publications

(17 citation statements)

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“…The significance of the 'H-labeled compounds with the mobility of cholylglycine is unknown. According to available evidence, neither chenodeoxycholic nor deoxycholic acid is hydroxylated in the liver of man (19)(20)(21), and if rehydroxylation occurred in our studies its magnitude was small; 7-rehydroxylation of deoxycholic acid does occur in the liver of several other species (22)(23)(24)(25).…”

Section: Resultsmentioning

confidence: 99%

Metabolism of steroid and amino acid moieties of conjugated bile acids in man

Hepner¹,

Hofmann²,

Thomas³

1972

J. Clin. Invest.

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A B S T R A C T Chenodeoxycholyl -2,4 -3H -glycine -1 -1C and deoxycholyl-2,4-3H-glycine-1-14C were synthesized and administered orally to 10 healthy subjects. Distribution of radioactivity among bile acids and specific activity of steroid and amino acid moieties were determined in bile samples. 3H and 14C were measured in feces.4C in breath was calculated from interval "CO2 specific activity determinations.The daily fractional turnover of the glycine moiety of chenodeoxycholyl and deoxycholylglycines was more than three times that of the steroid moiety. Pool size of chenodeoxycholylglycine was about twice that of deoxycholylglycine, but similar fractional turnover rates of steroid and amino acid moieties suggested that intestinal absorption of the two conjugated bile acids was equally efficient (about 95%). The amount of unlabeled deoxycholic acid (newly formed by bacterial 7a-dehydroxylation) absorbed from the intestine approximated 30% of the cholic acid that was lost. 3H radioactivity remained predominantly in administered bile acid implying that, normally, secondary bile acids derived from chenodeoxycholic acid are not appreciably absorbed from the intestine and that deoxycholic acid is not hydroxylated by the liver.Approximately 25% of administered 14C was recovered in the breath in the first 24 hr and less than 8% in the feces in 8 days; "CO2 excretion correlated highly with fractional turnover of the glycine moiety. 3H appeared predominantly in feces, and the rate of excretion correlated highly with the fractional turnover of the steroid moiety of bile acids. From the results in this paper plus previous measurements on the metabolism of cholylglycine, we calculated that about 6 mmoles/day of glycine is used for bile acid conjugation in health.

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Section: Resultsmentioning

confidence: 99%

Metabolism of steroid and amino acid moieties of conjugated bile acids in man

Hepner¹,

Hofmann²,

Thomas³

1972

J. Clin. Invest.

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“…These authors have further demonstrated that although nonconjugated cholic acid acts weakly to enhance esterification, deoxycholate, a bacterial product of cholic acid (16,17), is inhibitory. They have suggested that bacterial alteration of bile acid structure in the blind loop syndrome could lead to impaired fat absorption.…”

Section: Discussionmentioning

confidence: 99%

The role of altered bile acid metabolism in the steatorrhea of experimental blind loop.

Kim¹,

Spritz²,

Blum³

et al. 1966

J. Clin. Invest.

133

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“…According to published data, daily fecal excretion of bile acids varies from 87 mg (cholic acid metabolites only) to 2114 mg, depending on the methods of measurement used, and on the regimen given to the patient under study (23). If the average daily excretion of bile acids is assumed to be of 500 mg in an average adult, of this amount about 200 mg is deoxycholic acid and 300 mg is nonabsorbable lithocholic acid (24). Then, in the context of the present study.…”

Section: Discussionmentioning

confidence: 99%