In vitro free radicals scavenging activity and antioxidant capacity of solid-state fermented wheat bran and its potential modulation of antioxidative molecular targets in chicken PBMC

Abstract

-The purpose of this study was to demonstrate the in vitro free radical scavenging activity and antioxidant capacity in solid-state fermented wheat bran and its potential modulation of antioxidative molecular targets in chicken peripheral blood mononuclear cells (PBMC). After solid-state fermentation of wheat bran by white rot fungi for 12 d, the scavenging action of the fermented wheat bran extracts was 1, 1-diphenyl-2-picrylhydrazyl (DPPH), and the free radicals increased significantly, approximately 1.5-fold. Trolox equivalent antioxidant capacity of 1 mg/mL fermented wheat bran extracts was increased from 100 to 150 mM trolox antioxidant capacity after 12 d of fermentation. Moreover, the extracts exhibited 50% of the chelating capacity observed for ferrous iron (Fe2+) after fermenting for 12 d. In vitro, and under the stimulus of fermented wheat bran, the antioxidant gene expression (GST, HO-1, Nrf2, and GCLC genes) of PBMC was more than double that of the PBS, ascorbic acid, and unfermented wheat bran. The expression of fermented wheat bran was the lowest for the NOX1 and ROMO1 genes. Solid-state wheat bran fermented by white rot fungi can increase the scavenging action of DPPH, the trolox equivalent antioxidant capacity, and the chelating capacity of ferrous iron; in addition, in vitro, it can regulate the expression of antioxidant molecular targets in chicken PBMC.

Evaluation of Waste Mushroom Compost as a Feed Supplement and Its Effects on the Fat Metabolism and Antioxidant Capacity of Broilers

“…Mushrooms have superb antioxidant capacities and can increase antioxidant capacity in animals [5]. Wang et al [5,34] indicated that mushroom compost can improve the antioxidant capacity of poultry whether in vivo or in vitro. Animal antioxidant systems can be classified as enzymatic or non-enzymatic systems.…”

“…As a catalyst, HO-1 can indirectly reduce the harm caused by H 2 O 2 by cleaving hemoglobin into biliverdin [39]. Previous research determined that phenols have antioxidant capacities and will activate Nrf2 expression [40], therefore mushroom additives can improve the enzymatic antioxidant system and enhance animals' adaptability to oxidative stress [34]. In addition, as a phenolic compound, epigallocatechin (1.493 mg /g PWMC) can enhance the antioxidant capacity of animals by chelating metal ions or increasing antioxidant enzymes [29].…”

“…To achieve or exceed the nutritional requirements of the broilers, according to NRC 1994, all diets were recalculated and the proximate composition was analyzed according to the AOAC (2012) [14] to determine the gross energy and crude protein levels ( Table 1). The starter diet was provided for the first 21 days, and the finisher diet provided for days [22][23][24][25][26][27][28][29][30][31][32][33][34][35]. Body weight gain, feed intake, and feed conversion rate (FCR) were measured on 21-and 35-day-old broilers.…”

Effects of rice straw fermented with spent Pleurotus sajor-caju mushroom substrates on milking performance in Alpine dairy goats

“…Three experiments were conducted in this study, FRS in situ degradation, milking performance and rumen degradation of dairy goats fed FRS diet. Fistulated Holstein dry cows, Alpine lactating goats, and Alpine fistulated dry goats involved were raised in the for eight weeks in a dark room [15].…”

Assessment of The Performance of Chicks Fed with Wheat Bran Solid Fermented by Trichoderma longibrachiatum (SF1)

“…However, no difference was observed between birds treated with 10% wheat bran solid fermented by Trichoderma longibrachiatum (S1) and unfermented wheat bran group (control 2). Although wheat bran fermented by white rot fungi Pleurotus eryngii could hold the promise of antioxidant activity and its antioxidant capacity would be evaluated as free radical scavenging capacity (Wang et al, 2016). Concerning to (HI) test (immune response to New castle disease virus), results indicated that birds treated with 10% wheat bran fermented by Trichoderma longibrachiatum (S1) not significantly increased HI antibody response (256.0), when compared to control-1 treatment and unfermented wheat bran which recorded the same titre (256.0).…”