In vitro gastrointestinal digestion study of two wheat cultivars and evaluation of xylanase supplementation

Abstract

BackgroundThe filamentous fungus Talaromyces versatilis is known to improve the metabolizable energy of wheat-based poultry diets thanks to its ability to produce a pool of CAZymes and particularly endo-β(1,4)-xylanases. In order to appreciate their in vivo mode of action, the supplementation effect of two of its xylanases, XynD and XynB from families GH10 and GH11 respectively, have been evaluated on two different wheat cultivars Caphorn and Isengrain, which were chosen amongst 6 varieties for their difference in non starch polysaccharides content and arabinoxylan composition.ResultsPolysaccharides digestion was followed during 6 h along the digestive tract using the TNO gastrointestinal model-1, to mimic monogastric metabolism. Polysaccharide degradation appeared to occur mainly at the jejunal level and was higher with Isengrain than with Caphorn. For both cultivars, XynD and XynB supplementation increased notably the amount of reducing end sugars into the jejuno-ileal dialysates, which has been confirmed by a valuable increase of the soluble glucose into the jejunal dialysates.ConclusionsThe amounts of arabinose and xylose into the dialysates and ileal deliveries increased consequently mainly for Caphorn, suggesting that XynD and XynB supplementation in wheat-based diet could alleviate the anti-nutritional effects of arabinoxylans by limiting the physical entrapment of starch and could increase the available metabolizable energy.

Enzymology properties of two different xylanases and their impacts on growth performance and intestinal microflora of weaned piglets

“…This study used 3,5-Dinotrosalicylic acid (DNS) method and gave a comparative analysis on the change of xylanase from different sources when they in different temperatures and different pH. Xylanase supplementation in wheat-based diet could alleviate the anti-nutritional effects of arabinoxylans by limiting the physical entrapment of starch and could increase the available metabolizable energy ( Lafond et al., 2015 ). This study was to investigate the difference of different sources of xylanase enzyme properties, taking the Landrace × Large Whites × Duroc, as test animals to compare bacterial and fungal xylanase's different effects on the growth performance of pigs and the influence on intestinal microbial flora, at last, to provide evidence for xylanase's application in pig feed production.…”

Combined in situ Physical and ex-situ Biochemical Approaches to Investigate in vitro Deconstruction of Destarched Wheat Bran by Enzymes Cocktail Used in Animal Nutrition

“…Wheat bran AXs, which amounts to about 70% of the total NSPs, are linear chains of β-(1,4)-linked D-xylose on which α-L-arabinofuranosyl units are attached as single units on carbon 2 and /or carbon 3 of the xylosyl unit of linear chains (Ordaz-Ortiz and Saulnier, 2005; Knudsen, 2014). NSPs are recognized as anti-nutritional factors in animal nutrition because (a) they may trap nutrients (i.e., starch and proteins) within their fibrous structure, (b) they can act as chelators of minerals such as Ca 2+ or Fe 2+ , and (c) they can reduce nutrients adsorption due to their high molecular weight that enhances the viscosity in the digestive tract ion (Ravindran, 2013; Lafond et al, 2015). Therefore, since the 80th the common practice in animal nutrition has been to complement animal diets with enzymes cocktails (Bedford and Partrige, 2011).…”

“…Although enzymatic deconstruction of biomass is largely investigated in the field of biorefinery and biofuels (Himmel et al, 2007; Dodd and Cann, 2009; Kubicek and Kubicek, 2016), in feed nutrition, such studies are restricted to global effects on nutrient digestibility or nutrients intake (Aulrich and Flacoxwsky, 2001; Malathi and Devegowda, 2001; Vahjen et al, 2005; Brufau, 2006; Amerah, 2015; Lafond et al, 2015). Due to the heterogeneity and insolubility of wheat bran, unraveling the deconstruction of insoluble NSPs by NSPases is actually challenging, as it requires both physical tools to observe and monitor particles deconstruction and biochemical methods to identify and quantify the solubilized matter during the treatment.…”

Molecular Cloning and Characterizations of Xylanase Inhibitor Protein from Wheat (Triticum Aestivum)

“…Addition of the enzyme during gluten‐starch separation processes can improve the yield of starch (Hardt and others ). Addition of xylanase to feedstock has been used to remove the antinutritional effects of xylan and to enhance digestibility of silages (Lafond and others ; Munyaka and others ). Therefore, xylanase is widely used in food and feed industries as an additive (Pirgozliev and others ).…”