In vitro genotoxic effects of ZnO nanomaterials in human peripheral lymphocytes

Gümüş, Duygu; Berber, Ahmet Ali; Ada, Kezban; Aksoy, Hüseyin

doi:10.1007/s10616-013-9575-1

Cited by 19 publications

(12 citation statements)

References 68 publications

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“…41 –47 The previous studies reported that ZnO NPs induced DNA double-strand breaks frequency, olive tail moment, apoptosis, DNA breaks, and CA frequency on A549, HEK, HepG2, MEF Ogg1 +/+ , CHO, and human peripheral lymphocyte cells in the concentration range of 0.1 to 320 µg/mL. 23,48 –52 Similarly, our findings also demonstrated that ZnO NPs caused genotoxicity on human lymphocytes in the concentration range of 12.5 to 250 ppm. The ZnO NPs also caused cell death at 500 to 2000 ppm dose range; therefore, no cells were counted at these concentrations.…”

Section: Discussionmentioning

confidence: 98%

Investigation of the Genotoxicity of Aluminum Oxide, β-Tricalcium Phosphate, and Zinc Oxide Nanoparticles In Vitro

Akbaba

Türkez

2018

Int J Toxicol

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The aim of this study was to investigate the genotoxicity of aluminum oxide (AlO), β-tricalcium phosphate (β-TCP) (Ca(PO)), and zinc oxide (ZnO) nanoparticles (NPs) that were 4.175, 9.058, and 19.8 nm sized, respectively, on human peripheral blood lymphocytes using micronucleus (MN) and chromosome aberration (CA) techniques. Aluminum oxide and β-TCP NPs did not show genotoxic effects on human peripheral blood cultures in vitro, even at the highest concentrations; therefore, these materials may be suitable for use as biocompatible materials. It was observed that, even at a very low dose (≥12.5 ppm), ZnO NPs had led to genotoxicity. In addition, at high concentrations (500 ppm and above), ZnO NPs caused mortality of lymphocytes. For these reasons, it was concluded that ZnO NPs are not appropriate for using as a biocompatible biomaterial.

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Section: Discussionmentioning

confidence: 98%

Investigation of the Genotoxicity of Aluminum Oxide, β-Tricalcium Phosphate, and Zinc Oxide Nanoparticles In Vitro

Akbaba

Türkez

2018

Int J Toxicol

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“…When investigated in cultured human peripheral lymphocytes, ZnO NPs significantly increased the MN frequency at concentrations of 10 and 15 g/mL compared with that in the control. However, lower concentrations (<10 g/mL) did not induce significant alterations in the MN frequency ( Gümüs et al, 2014). ZnO NPs and its bulky forms evaluated in human embryonic kidney (HEK293) and mouse embryonic fibroblast (NIH/3T3) cells were able to increase significantly the number of MNs at doses up 100 µg/mL (Demir et al, 2014).…”

Section: A C C E P T E D Accepted Manuscriptmentioning

confidence: 91%

“…The authors suggested that this could be due to an increased aggregation of ZnO NPs at high concentrations in the lymphocyte culture. Therefore, the addition of increasing concentrations/doses of ZnO NPs to lymphocyte cultures may not be paralleled by an equivalent increase in the genotoxic effects of this nanomaterial ( Gümüs et al, 2014).…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…The in vitro MN assay can detect chromosomal damage in the form of clastogenicity induced by ROS, one of the main mechanisms of NP genotoxicity (Pfuhler et al, 2013). The CBMN has been widely accepted to screen ZnO NPs for genotoxicity in different cell lines (Osman et al, 2010;Corradi et al, 2012;Wahab et al, 2013;Bhattacharya et al, 2014;Demir et al, 2014;Gümüs et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Assessment of the genotoxic potential of two zinc oxide sources (amorphous and nanoparticles) using the in vitro micronucleus test and the in vivo wing somatic mutation and recombination test

Reis

Rezende

Santos

et al. 2015

Food and Chemical Toxicology

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In this study, we evaluated the toxic and genotoxic potential of zinc oxide nanoparticles (ZnO NPs) of 20 nm and the mutagenic potential of these ZnO NPs as well as that of an amorphous ZnO. Toxicity was assessed by XTT colorimetric assay. ZnO NPs were toxic at concentrations equal to or higher than 240.0 μM. Genotoxicity was assessed by in vitro Cytokinesis Block Micronucleus Assay (CBMN) in V79 cells. ZnO NPs were genotoxic at 120.0 μM. The mutagenic potential of amorphous ZnO and the ZnO NPs was assayed using the wing Somatic Mutation and Recombination Test (SMART) of Drosophila melanogaster. In the Standard cross, the amorphous ZnO and ZnO NPs were not mutagenic. Nevertheless, Marker trans-heterozygous individuals from the High bioactivation cross treated with amorphous ZnO (6.25 mM) and ZnO NPs (12.50 mM) displayed a significant increased number of mutant spots when compared with the negative control. In conclusion, the results were not dose related and indicate that only higher concentrations of ZnO NPs were toxic and able to induce genotoxicity in V79 cells. The increase in mutant spots observed in D. melanogaster was generated due to mitotic recombination, rather than mutational events.

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“…Although selective cytotoxic effects on lymphocytes is an immunotoxic effect most frequently associated with immunosuppressive responses, a similar effect could be associated with implications for allergic conditions, as well (1121). Accordingly, metal nanomaterial cytotoxic and genotoxic effects have been investigated in murine and human lymphocytes in vitro and correlated to properties including size and morphology (1122)(1123)(1124)(1125)(1126)(1127)(1128). Interestingly, lymphocytes have been demonstrated to be more resistant to adverse effects of ZnONP compared to other immune cell types (1129).…”

Section: Metal Nanomaterials Effects On Processes Involved In Allergicmentioning

confidence: 99%

Metal Nanomaterials: Immune Effects and Implications of Physicochemical Properties on Sensitization, Elicitation, and Augmentation of Allergic Disease

Roach¹

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The recent surge in incorporation of engineered metallic and metal oxide nanomaterials into consumer products and their corresponding use in occupational settings has raised concerns over their potential to induce material size-specific toxicological effects. Although metal nanomaterials have been shown to induce greater degrees of lung injury and inflammation than their larger metal counterparts, their size-related effects on the immune system and allergic disease remain largely unknown. This knowledge gap is particularly concerning since metals are common inducers of allergic contact dermatitis, occupational asthma, and allergic adjuvancy.Overall, more scientific knowledge exists regarding the potential for metal-containing nanomaterials to exacerbate allergic disease than to their potential to induce allergic disease. Furthermore, effects of metal nanomaterial exposure on respiratory allergy have been more thoroughly-characterized than their potential influence on dermal allergy. Despite the existence of such knowledge, specific correlations between metal nanomaterial physico-chemical properties and their allergic effects have yet to be consistently demonstrated. As the number of emerging nanomaterials continues to increase, the delineation of these relationships has the potential to advance risk assessment efforts by helping to identify specific agents that may present an elevated risk for allergic effects.Two sets of studies were designed to begin addressing some of the knowledge gaps associated with the immunotoxic potential of metal nanomaterials in the context of allergic disease. The hypothesis of these studies was that nanomaterials comprised of metal constituents with known immunomodulatory potential augment, induce, and elicit allergic disease more readily than larger forms of the materials. Moreover, the nature of the immune responses caused by exposure to these metal nanomaterials, as well as the magnitude of the effects, correlates best with the parameter of dose surface area.The first set of studies incorporated NiO particles with different physico-chemical properties into an in vivo time course study and OVA asthma model. Results from the time course study demonstrated that the smaller NiO particles caused more pronounced pulmonary injury and inflammation, a discrepancy that could be mitigated by normalizing the administered particle dose to the surface area of the larger material. In the OVA model, augmentation of the allergic response was largely conserved with respect to NiO surface area, wherein larger doses caused polarization of pulmonary immune reposes towards a Th1/17-dominant state and smaller doses induced Th2-skewed responses. Despite this association, several immune markers, including total IgE production, BAL eosinophil number, and Penh response correlated better with other metrics, such as particle size.The second set of studies employed Au particles and nanoparticles to assess size-specific differences in allergenic potential. Neither particle was associated with potential for skin...

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In vitro genotoxic effects of ZnO nanomaterials in human peripheral lymphocytes

Cited by 19 publications

References 68 publications

Investigation of the Genotoxicity of Aluminum Oxide, β-Tricalcium Phosphate, and Zinc Oxide Nanoparticles In Vitro

Investigation of the Genotoxicity of Aluminum Oxide, β-Tricalcium Phosphate, and Zinc Oxide Nanoparticles In Vitro

Assessment of the genotoxic potential of two zinc oxide sources (amorphous and nanoparticles) using the in vitro micronucleus test and the in vivo wing somatic mutation and recombination test

Metal Nanomaterials: Immune Effects and Implications of Physicochemical Properties on Sensitization, Elicitation, and Augmentation of Allergic Disease

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