In vitro genotoxic evaluation of the medicinal plant Chenopodium ambrosioides L.

Gadano, Andrea; Gurni, Alberto A.; López, Paula; Ferraro, Graciela; Carballo, Marta A.

doi:10.1016/s0378-8741(01)00418-4

Cited by 90 publications

(64 citation statements)

References 12 publications

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“…In vivo studies showed that exposure of mice bone marrow cells to the alcoholic extracts of black pepper (Madrigal-Bujaidar et al 1997) and Catha edulis , as well as the major alkaloid of betel nut, arecoline (Chatterjee and Deb 1999), increased the frequency of SCE formation in these cells. Similarly, in vitro studies demonstrated that treatment with the aqueous extracts of C. ambrosioides L. (Gadano et al 2002(Gadano et al , 2006, C. multifidum L. (Gadano et al 2006), and Helleborus niger (Bussing and Schweizer 1998), the alcoholic extract of black pepper (Madrigal-Bujaidar et al 1997), and both the ethanolic and aqueous extracts of Geranium macrorrhizum (Venskutonis et al 2010) induced SCE formation in human lymphocyte cell cultures (Lazutka et al 2001). Also, treatment with the ethanolic extract of Gardenia jasminoides (Ozaki et al 2002) and the aqueous extract of betel quid (Wang et al 1999) increased the frequency of SCE formation in V79 and ovary K1 hamster cells, respectively.…”

Section: Discussionmentioning

confidence: 92%

Genotoxic and cytotoxic effects ofArtemisia herba-albaon mammalian cells

Abderrahman

Shbailat

2014

Caryologia

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Artemisia herba-alba is a medicinal plant that is commonly used in folk remedies in the Middle East. Despite its wide use, little is known about whether or not it is genotoxic and/or cytotoxic to mammalian cells. Here we investigated the genotoxic and cytotoxic effects of the ethanolic plant extract on mice cells at different dose concentrations (125, 250, 375, and 500 μg ml ) and for different exposure times (12, 24, 48, and 72 h). We found that at all exposure times, treatment with high dose concentrations (375 and 500 μg ml ) of A. herba-alba extract significantly reduced cell division in mice bone marrow cells compared to that in the control cells. Also, it induced sister chromatid exchanges and micronuclei formation in bone marrow and peripheral blood cells, respectively. Moreover, high dose concentrations significantly reduced cell viability in bone marrow cell cultures compared to that in the controls. The IC 50 values were 484.38, 513.33, and 490.39 μg ml −1 after 24, 48, and 72 h of exposure, respectively. Our findings reveal that A. herba-alba extract has both genotoxic and cytotoxic effects on mammalian cells, particularly at high concentrations. Therefore, we suggest that the plant extract should not be used at high concentrations in folk medicine.

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Section: Discussionmentioning

confidence: 92%

Genotoxic and cytotoxic effects ofArtemisia herba-albaon mammalian cells

Abderrahman

Shbailat

2014

Caryologia

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“…In this context, several plants and constituent bioactive substances, also called insecticides of plant origin or botanical insecticides, have been tested and considered promising in the control of cowpea beetle (Denloye et al, 2010, Kheradmand et al, 2010, Udo, 2011. Chenopodium ambrosioides Linn and Aframomum melegueta K. Schum were chosen because they have shown promise of medicinal and insecticidal activity in Malawi, Central Africa countries such as Cameroon and Gabon and Southern Africa (Gadano, 2002, Tapondjou et al, 2002, Konning et al, 2004Okwu, 2005, Odugbemi, 2006, Denloye et al, 2009. This study aimed to assess insecticide effect of C. ambrosioides leaf and A. melegueta seed powders against C. maculatus during in stored cowpea.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of two plant powders as cowpea grain protectants against <i>Callosobruchus maculatus</i> Fabricius (Coleoptera, Chrysomelideae: Bruchinae)

Chougourou¹,

Agossa²,

Zoclanclounon³

et al. 2016

J. App. Bioscience.

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Objective: This study was carried out to study insecticidal effect of powders from Chenopodium ambrosioides leaves and Aframomum melegueta seeds on cowpea weevil Callosobruchus maculatus. Methodology and Results: 5g of both powders of A. melegueta and C. ambrosioides were used in the proportions of C. ambrosioides 100%:A. melegueta 0%, C. ambrosioides 0%: A. melegueta 100%, C. ambrosioides 30%: A. melegueta 70%, C. ambrosioides 70%: A. melegueta 30% and C. ambrosioides 50%: A. melegueta 50%. Plant powders were added to 20g of cowpea grains. Bioassays were conducted at 26.64±0.74°C and 72.55±4.38% relative humidity. Insect mortality was evaluated from 24 to 144 hours after treatment. Results obtained indicated that plant powders had significant effect on C. maculatus mortality. The highest mortality rate (70.00±26.45%) was recorded in jar treated with C. ambrosioides. Conclusion and application of findings: Either plant powders, alone or mixed had high insecticidal effect on C. maculatus. Because of their effectiveness, these plant powders could be recommended as grain protectants against C. maculatus.

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“…Para isso são utilizados testes que servem como biomarcadores, os quais são úteis e podem ser definidos como sistemas indicadores que geralmente incluem subsistemas de um organismo completo, usados para identificação de um alvo específico (SILVA et al, 2003). O índice mitótico e o índice de replicação podem ser utilizados como indicativos da proliferação adequada das células, e podem ser medidos através do sistema teste vegetal de Allium cepa L. (GADANO et al, 2002). Este tem sido amplamente utilizado como bioindicador de genotoxicidade (TEDESCO & LAGHINGHOUSE, 2012).…”

Section: Introductionunclassified

ANÁLISE PROLIFERATIVA E FITOQUÍMICA DE Commelina erecta L. PELO TESTE Allium cepa L.

Trapp¹,

Pasqualli²,

Rodrigues³

et al. 2016

Enci Bio

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RESUMOCommelina erecta L., popularmente conhecida como trapoeraba, é tida como invasora de culturas tradicionais causando danos econômicos. Infusões preparadas a partir de suas folhas são utilizadas no tratamento de inflamações, principalmente as relacionadas aos olhos. Por ser utilizada como planta medicinal, surge a necessidade de estudos de genotoxicidade que podem ser realizados através do teste de Allium cepa L., e estudos fitoquímicos para identificação de compostos presentes nas infusões. Os objetivos do trabalho foram verificar o potencial antiproliferativo das infusões de folhas (frescas -2.0 g.L -1 e secas -0,64 g.L ) de C. erecta, e realizar sua análise fitoquímica. Foram utilizados seis grupos de quatro bulbos de A. cepa, cada grupo correspondendo a um tratamento. A água de torneira foi utilizada como controle negativo e o glifosato 1,5% como controle positivo. Os bulbos foram enraizados primeiramente em água destilada e posteriormente transferidos para os tratamentos onde permaneceram por 24 horas. Após, as raízes foram coletadas e fixadas em Carnoy 3:1 e armazenadas sob refrigeração em etanol 70%. As lâminas foram preparadas pela técnica de esmagamento, sendo analisadas 4.000 células por tratamento e calculados os índices mitóticos. A análise estatística foi realizada pelo teste χ 2 . A identificação dos compostos presentes nas infusões foi realizada pela técnica de Cromatografia Líquida de Alta Eficiência (CLAE). Os resultados demonstraram capacidade antiproliferativa das infusões de C. erecta e presença de quercetina e isoquercitrina como compostos majoritários. PALAVRAS-CHAVE: bioindicador, plantas medicinais. Trapoeraba. PROLIFERATIVE AND PHYTOCHEMISTRY ANALYSIS OF Commelina erecta L.BY Allium cepa L. TEST ABSTRACT Commelina erecta L., popularly known as spiderwort, it is considered invasive of traditional crops causing economic damage. Infusions prepared from your leaves are

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In vitro genotoxic evaluation of the medicinal plant Chenopodium ambrosioides L.

Cited by 90 publications

References 12 publications

Genotoxic and cytotoxic effects ofArtemisia herba-albaon mammalian cells

Genotoxic and cytotoxic effects ofArtemisia herba-albaon mammalian cells

Efficacy of two plant powders as cowpea grain protectants against <i>Callosobruchus maculatus</i> Fabricius (Coleoptera, Chrysomelideae: Bruchinae)

ANÁLISE PROLIFERATIVA E FITOQUÍMICA DE Commelina erecta L. PELO TESTE Allium cepa L.

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