In vitro growth inhibition of major mastitis pathogens by Staphylococcus chromogenes originating from teat apices of dairy heifers

Vliegher, Sarne De; Opsomer, G.; Vanrolleghem, A; Devriese, Luc; Sampimon, O.C.; Sol, J.; Barkema, Herman W.; Haesebrouck, Freddy; Kruif, Aart de

doi:10.1016/j.vetmic.2004.03.020

Cited by 87 publications

(95 citation statements)

References 15 publications

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“…The direct toxic effects of certain bacterial species against other ones invading the host have also been considered, since flora populations can secure their domination over invading pathogens by producing antibacterial substances [9,26]. Staphylococcal strains isolated from the cows' teat orifice or mammary secretion have been found to produce bacteriocins and reduce in vitro growth of other pathogens [10,44].…”

Section: Discussionmentioning

confidence: 99%

The bacterial flora in the teat duct of ewes can protect against and can cause mastitis

et al. 2007

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-We studied the possible effects of bacterial populations within the teat duct, in the pathogenesis of ovine mastitis. In experiment I, 32 ewes were allocated into group A (ewes from which we isolated (+++ growth) coagulase-negative staphylococci), B (ewes from whose duct we isolated (+ growth) coagulase-negative staphylococci) or C (ewes from which we isolated Bacillus spp.) and subdivided into A1, B1, C1 (n = 4; challenged by deposition of 1.250 cfu of Mannheimia haemolytica into the teat duct) or A2, B2, C2 (n = 4; used as uninoculated controls); group D (n = 8) contained ewes with no bacteria in their teat ducts and were challenged as above. There were less bacteriological isolations of flora (P = 0.018) and challenge (P < 0.05) organisms from A1 than from A2 and D ewes; the severity of pathological findings in A1 (summed up score: 27) ewes was smaller than in D (summed up score: 36) ewes (P = 0.038). No such findings were evident with B1 or C1 ewes (P > 0.4). In experiment II, ewes (groups E and F, n = 6) from whose duct we isolated coagulase-negative staphylococci (+ growth) were used; in group G (n = 6) ewes with no bacteria in their teat ducts were included. Teat chapping was applied in E and G ewes. All E ewes developed acute clinical mastitis within 24 h after teat chapping, although we had carried out no challenge; there were more bacteriological isolations of flora organisms from E than from F and G ewes (P < 0.001); the severity of pathological findings in E (score: 28) was greater than in F (score: 3) or G (score: 14) ewes. In experiment III, eight ewes with no bacteria in their teat ducts were allocated into group H or I (n = 4) and challenged into the teat (group H) or into the gland (group I) with 10 6 cfu of a Staphylococcus simulans recovered from the teat duct of a group E ewe. Group H ewes developed transiently clinical followed by subclinical mastitis (based on bacteriological and cytological evidence), whilst group I ewes developed severe clinical disease. We conclude that staphylococcal flora present in high numbers within the teat duct of ewes can afford some protection against invading microorganisms. However with impeded defence mechanisms of the teat, the same flora may invade the mammary parenchyma and cause clinical mastitis. mastitis / sheep / teat / predisposing factor / bacterial flora

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Section: Discussionmentioning

confidence: 99%

The bacterial flora in the teat duct of ewes can protect against and can cause mastitis

et al. 2007

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“…In the second experiment, 2 additional bovine CNS strains were included [a Staphylococcus fleurettii strain originating from sawdust (Supré et al, 2011) and a S. chromogenes strain originating from a teat apex (TA) of a heifer (De Vliegher et al, 2004)]. The fourth gland pair of each of the 18 mice (n mice/CNS strain = 6; n glands/ CNS strain = 12) were injected with either S. chromogenes IM, S. chromogenes TA, or S. fleurettii at an inoculum dose of approx.…”

Section: Technical Notementioning

confidence: 99%

Technical note: A pilot study using a mouse mastitis model to study differences between bovine associated coagulase-negative staphylococci

Breyne

Vliegher

Visscher

et al. 2015

Journal of Dairy Science

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“…Additionally, NAS contain important virulence factors (Zhang et al, 2003; Otto, 2013; Vanderhaeghen et al, 2014), have a high level of antimicrobial resistance (Rajala-Schultz et al, 2009; Frey et al, 2013; Taponen et al, 2015), and can cause chronic IMI (Gillespie et al, 2009; De Vliegher et al, 2012). In contrast, NAS have also been reported to have a protective role against major IMI-related pathogens (Matthews et al, 1990; De Vliegher et al, 2004). Contradictory findings among studies regarding pathogenicity of NAS is potentially related to classifying disparate species and strains of staphylococci as one group (Woodward et al, 1987, 1988; Matthews et al, 1990; Piepers et al, 2009; Vanderhaeghen et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Phylogenetic Analysis of Bovine Non-aureus Staphylococci Species Based on Whole-Genome Sequencing

et al. 2016

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Non-aureus staphylococci (NAS), a heterogeneous group of a large number of species and subspecies, are the most frequently isolated pathogens from intramammary infections in dairy cattle. Phylogenetic relationships among bovine NAS species are controversial and have mostly been determined based on single-gene trees. Herein, we analyzed phylogeny of bovine NAS species using whole-genome sequencing (WGS) of 441 distinct isolates. In addition, evolutionary relationships among bovine NAS were estimated from multilocus data of 16S rRNA, hsp60, rpoB, sodA, and tuf genes and sequences from these and numerous other single genes/proteins. All phylogenies were created with FastTree, Maximum-Likelihood, Maximum-Parsimony, and Neighbor-Joining methods. Regardless of methodology, WGS-trees clearly separated bovine NAS species into five monophyletic coherent clades. Furthermore, there were consistent interspecies relationships within clades in all WGS phylogenetic reconstructions. Except for the Maximum-Parsimony tree, multilocus data analysis similarly produced five clades. There were large variations in determining clades and interspecies relationships in single gene/protein trees, under different methods of tree constructions, highlighting limitations of using single genes for determining bovine NAS phylogeny. However, based on WGS data, we established a robust phylogeny of bovine NAS species, unaffected by method or model of evolutionary reconstructions. Therefore, it is now possible to determine associations between phylogeny and many biological traits, such as virulence, antimicrobial resistance, environmental niche, geographical distribution, and host specificity.

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In vitro growth inhibition of major mastitis pathogens by Staphylococcus chromogenes originating from teat apices of dairy heifers

Cited by 87 publications

References 15 publications

The bacterial flora in the teat duct of ewes can protect against and can cause mastitis

The bacterial flora in the teat duct of ewes can protect against and can cause mastitis

Technical note: A pilot study using a mouse mastitis model to study differences between bovine associated coagulase-negative staphylococci

Comprehensive Phylogenetic Analysis of Bovine Non-aureus Staphylococci Species Based on Whole-Genome Sequencing

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