In vitro growth of buds taken from seedlings and adult plant material in Quercus robur L.

Abstract

In vitro growth of Quercus robur L. buds taken from 1-7 month old seedlings or adult plant material was studied. The following were investigated for their effects on the establishment of explants and subsequent subcul~res: original position and lignification of the primary explant, conditioning and ageing of source plants, incorporation of N 6 benzyladenine (BA) and activated charcoal in the medium.For bud break the best results were obtained with explants from herbaceous twigs in all tested media. For shoot growth the results depended on the medium used. Medium containing activated charcoal produced episodic growth, leaf organogenesis was reduced, spontaneous rooting occurred, but subculturing from adult plant material failed. On medium containing more than 8.8/aM of BA, all the buds developed abnormally and elongation did not occur. At lower concentrations of BA (4.4/aM) shoots elongated, leaf organogenesis was increased and episodic growth tended to disappear on subcultured seedling explants. No spontaneous rooting was observed, but subculture from adult plant material was managed successfully.

Influence of virus and virus-like agents on the development of citrus buds cultured in vitro

“…The importance of the overall quality and characteristics of the explant source is generally taken into consideration and has been the object of comprehensive reviews and articles [ 11,18,28]. However, whereas the deleterious effect of fungal and bacterial contaminants has been the object of attention [10,14,17,30], there is no information available on the effect of virus and virus-like infection.…”

Vegetative propagation of oak (Quercus robur and Q petraea) by cutting and tissue culture

“…Micropropagated plantlets were transplanted into soil and later were planted in the field. The system of axillary-shoot multiplication was used for micropropagation of various oak species: Q robur and Q petraea (Chalupa, 1979(Chalupa, , 1981(Chalupa, , 1983(Chalupa, , 1984(Chalupa, , 1985(Chalupa, , 1987b(Chalupa, , 1988(Chalupa, , 1990bVietez et al 1985;Pevalek-Kozlina and Jelaska 1986;Civinová and Sladky, 1987;Favre and Juncker, 1987;Meier-Dinkel, 1987;SanJosé et al 1988SanJosé et al , 1990Juncker and Favre, 1989;Volkaert et al, 1990), Q suber (Bellarosa, 1981(Bellarosa, , 1989Pardos, 1981; Manzanera and Pardos, 1990), Q Shumardii (Bennett and Davies, 1986), Q acutissima (Ide and Yamamoto, 1986;Sato et al, 1987), Q serrata (Ide and Yamamoto, 1987) and Q lobata (Johnson and Walker, 1990 (Chalupa, 1985(Chalupa, , 1987a(Chalupa, , 1990cJörgensen, 1988), Q suber (El Maataoui and Espagnac, 1987), Q acutissima (Sasaki et al, 1988), Q rubra and Q alba (Gingas and Lineberger, 1989) (Gresshoff and Doy, 1972), BTM (Chalupa, 1984), or Woody plant medium (WPM) (Lloyd and McCown, 1980). The basal media were supplemented with glutamine (100 mg·l -1 ).…”

Long-term effects of culture establishment from shoot-tip explants in micropropagating oak (Quercus robur L)

“…In Quercus robur, in vitro propagation from stem explants has been achieved (Chalupa, 1984(Chalupa, , 1988(Chalupa, , 1993Vieitez et al, 1985;Favre and Juncker, 1987;MeierDinkel, 1987;San-Jose et al, 1988;MeierDinkel et al, 1993 Slak and Favre, 1990 Gresshoff and Doy, 1972) However, these indications needed to be considered with care because of possible interference of clonal effects ). …”