In vitro Growth of Genovese Basil in Response to Different Concentrations of Salts and Interaction of Sucrose and Activated Carbon

Trettel, Jéssica Rezende; Nascimento, Andressa Bezerra; Barbosa, Letícia Neris; Magalhães, Hélida Mara

doi:10.5539/jas.v10n9p142

Cited by 4 publications

(9 citation statements)

References 26 publications

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“…In this case, the actived charcoal did not restrict Cu and Zn uptake, as M3 and M5 media had higher amounts of these micronutrients (Table 4). These results corroborate those reported by Trettel et al (2018b), where Ocimum basilicum plantlets maintained in MS medium with 60 g L -1 of sucrose and 4.5 g L -1 of actived charcoal did not show an increased plantlets growth, and these micronutrients did not contribute to adsorption of Cu and Zn.…”

Section: Different Compositions Of Ms Mediumsupporting

confidence: 92%

Growth Regulators Affect the Growth and Biochemical Activity of Curcuma longa Plants Grown in vitro

Ferrari¹,

Queiroz²,

Andrade³

et al. 2019

JAS

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This study aimed to evaluate the in vitro growth and biochemical activity of Curcuma longa explants using different MS medium formulations and growth regulators. In all the experiments, plants were grown in MS medium supplemented with agar (6.5 g L-1) and pH adjusted to 5.8. In the first assay, the MS culture medium at 70% strength, supplemented with 30 g L-1 of sucrose, and without the addition of activated carbon, resulted in the highest number of shoots. The sucrose concentration of 60 g L-1, combined with the addition of actived charcoalin half-strength MS medium, resulted in the increased root dry mass, root collar diameter, and relative chlorophyll index. In the second assay, the highest root collar diameter and dry matter of shoots and roots were found in the MS medium supplemented with 4.44 BAP, 0.46 KIN, and 1.08 NAA. The MS medium with 8.88 BAP, 0.92 KIN, and 2.16 μM NAA resulted in the highest number of shoots (7.75), number of leaves (35), and shoot length (88.57 mm). The antioxidant activity was significantly higher in the treatments that resulted in better plantlets growth performance, demonstrating that the antioxidant activity is related to other factors such as a possible role of growth regulators on the elicitation of compounds in plants. Superoxide dismutase had a high enzymatic activity in both assays, whereas the enzymatic activity of catalase and ascorbate peroxidase was dependent on the culture media used.

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Section: Different Compositions Of Ms Mediumsupporting

confidence: 92%

Growth Regulators Affect the Growth and Biochemical Activity of Curcuma longa Plants Grown in vitro

Ferrari¹,

Queiroz²,

Andrade³

et al. 2019

JAS

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“…The higher amounts of macronutrients, especially the nitrogen associated with the plant growth regulators (Table 3), promoted an increase in the number of leaves in the T4 medium (Table 2). Studies have reported that O. basilicum plants are responsive to high amounts of nitrogen and plant growth regulators to promote leaf emission (SILVA et al, 2017;TRETTEL et al, 2018a). Nitrogen is a constituent of the chlorophyll molecule (GREENWAY et al, 2012) and plant growth regulators, mainly cytokinins, contribute to the expression of genes that act on the cell cycle and promote leaf emission (TANK; THAKER, 2011; NEELAKANDAN; WANG, 2012).…”

Section: Resultsmentioning

confidence: 99%

“…Regarding the root growth of O. basilicum seedlings and other species of the Lamiaceae family, it has been verified that a reduction in nutrient concentration in the culture medium favored the growth and mass production of the root system. For example, the 'Genovese' and 'Basilicão Roxo' basil cultivars exposed to a 70-80% saline concentration showed an increase in root length and mass (SILVA et al, 2017;TRETTEL et al, 2018a). For other species of the Lamiaceae family, a reduction of up to 50% in salts favored root system growth (FADEL et al, 2010;SWAMY;MOHANTY;ANURADHA, 2014).…”

Section: Resultsmentioning

confidence: 99%

“…The growth regulators used were 6-benzylaminopurine (BAP) and αnaphthaleneacetic acid (NAA; Sigma Aldrich ® ,Darmastadt, Germany). The treatments in this bioassay were based on previous studies by Silva et al (2017) and Trettel et al (2018a).…”

Section: Composition Of the Culture Medium For Sweet Basil Developmentmentioning

confidence: 99%

“…The material was then kept in a growth chamber for 80 days at 25 °C (± 2 °C). The photoperiod was 24 h (SILVA et al 2017;TRETTEL et al 2018a) and white fluorescent 20-W lamps (Empalux ® , Curitiba, Brazil) at a light intensity of 72.0 μmol m −2 s −1 were used.…”

Section: Composition Of the Culture Medium For Sweet Basil Developmentmentioning

confidence: 99%

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Growth, Enzymatic Activity, and Antioxidant Activity of Sweet Basil Grown in Vitro

et al. 2020

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Sweet basil is a perennial herb. Studies on in vitro cultivation of these plant species are scarce and inconclusive. This study was carried out to investigate the effect of culture medium concentration in combination with antioxidants and plant growth regulators on the in vitro growth and biochemical activity of sweet basil seedlings. Seeds of the ‘Genovese’ cultivar were inoculated into Murashige and Skoog culture medium supplemented with activated carbon and plant growth regulators 6 -benzylaminopurine and a-naphthaleneacetic acid. The seedlings were grown under controlled conditions for 80 days and their biometric and biochemical characteristics evaluated. More abnormal seedlings were observed in the 100% medium with 30 g L-1 sucrose, 0.4 g L-1 6-benzylaminopurine, and 0.2 g L-1 a-naphthaleneacetic acid (T4) and the medium without regulators (T1). However, the T4 culture medium resulted in a higher leaf number and shoot dry mass. Antioxidant activity was higher in the seedlings grown in the culture medium composed of 100% medium + 3.0 g L-1 activated carbon + 0.4 mg L-1 6-benzylaminopurine + 0.2 mg L-1 a-naphthaleneacetic acid (T5) and that composed of 70% medium + 3.0 g L-1 activated carbon + 0.1 mg L-1 6-benzylaminopurine (T3). The enzyme superoxide dismutase showed higher activity in all culture media than catalase or ascorbate peroxidase. Sweet basil seedlings growing in T4 and T1 medium showed the highest growth rate of shoots and the lowest antioxidant activity, whereas seedlings grown in T3 medium had the highest catalase and ascorbate peroxidase activity.

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Biostimulant effect of native Trichoderma strains on the germination of four varieties of basil

Hollman-Aragón,

Romero-Bastidas,

Arce-Amezquita

et al. 2023

RMF, MJP

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Objetive/antecedents. Trichoderma is an efficient tool as biostimulant in basil crop. However, only few species have been studied in specific cultivars. Therefore, the objective of this research was to evaluate the biostimulant efficacy of native Trichoderma strains on the germination and growth of four varieties of basil. Materials and Methods. Seven strains of Trichoderma (T. asperellum, atroviride, viride, longibrachiatum, harzianum, koningii and Trichoderma sp.), a commercial Trichoderma (T. harzianum), synthetic fertilizer (T17) and the control were used in the study. 30 seeds of the Purple Ruffles, Lemon, Siam Queen and Nufar varieties were treated with a spore suspension of each Trichoderma. 48 h later, the seeds were sown and incubated at 28 °C with a 12 h light/dark photoperiod. The variables evaluated were; Rate and percentage of germination, biomass and length of seedlings. Results. T. atroviride presented the greatest biostimulant effect on germination (95%). While T. asperellum registered an increased efficiency in biomass (≥ 0.120 g) and length (≥ 1.0 cm) of the plant in the four varieties. The action of commercial T. was lower in all cases. Conclusion. This study demonstrated that the native strains of Trichoderma have a biostimulant effect on plants and are more effective than commercial species.

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In vitro Growth of Genovese Basil in Response to Different Concentrations of Salts and Interaction of Sucrose and Activated Carbon

Cited by 4 publications

References 26 publications

Growth Regulators Affect the Growth and Biochemical Activity of Curcuma longa Plants Grown in vitro

Growth Regulators Affect the Growth and Biochemical Activity of Curcuma longa Plants Grown in vitro

Growth, Enzymatic Activity, and Antioxidant Activity of Sweet Basil Grown in Vitro

Biostimulant effect of native Trichoderma strains on the germination of four varieties of basil

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