In vitro high frequency multiplication and assessment of genetic fidelity of Corallocarpus epigaeus: an endangered medicinal plant

Vemula, Suresh; Koppula, Thirupathi; Jogam, Phanikanth; Mohammed, Mustafa S.

doi:10.1007/s42535-019-00085-6

Cited by 18 publications

(4 citation statements)

References 40 publications

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“…1e and Table 1). However, the regeneration studies carried out previously in the same plant showed the maximum percentage response of 68.33 on BA (0.5 mg/l) + IAA (2.0 mg/l) (Narayan 2016) and 88 on TDZ (1.5 mg/l) + IAA (1.5 mg/l) (Vemula et al 2020). The combination of IBA and BAP was also found to be effective in species like Bryonopsis lacinosa, where IBA and BAP induced multiple shoots from cotyledon derived callus (Caroline and Mallaiah 2011), whereas in Cicer arietinum, IBA and BAP resulted in maximum percentage of response in all explants (Sadhu et al 2020).…”

Section: Resultsmentioning

confidence: 77%

“…As per the above reason, it is very essential for conservation and in vitro propagation. The in vitro propagation studies were carried out in C. epigaeus for protection of species with the help of leaf and node explants, due to lack of a suitable method for natural regeneration and over-exploitation of C. epigaeus, which severely deteriorated the species consequently recording them as endangered species (Vemula et al 2020). Due to exploitative harvesting of tubers for trade and denudation of forests, the natural population of C. epigaeus has declined to such an extent that it is now considered rare and threatened in its natural habitats (Narayan 2016).…”

Section: Introductionmentioning

confidence: 99%

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High Efficiency In vitro Regeneration and Genetic Stability of Corallocarpus epigaeus - An Endangered Medicinal Plant

Veera

Chirumamilla²,

Taduri

2020

Plant Tissue Cult. & Biotech.

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Indirect regeneration of plantlets from multiple shoot induction of Corallocarpus epigaeus was obtained from leaf and nodal explants on MS with different concentrations of BAP in combination with IAA/IBA or IBA alone. Among all the combinations, BAP and IBA exhibited maximum regeneration. High frequency of multiple shoots (89%) was obtained on BAP (2.0 mg/l) and IBA (1.5 mg/l) in nodal explants. Maximum mean shoot length of 6.8 ± 0.33 cm was obtained in nodal explants cultured on BAP (1.0 mg/l) + IBA (0.5 mg/l), followed by leaf explants with 6.7 ± 0.47 cm on BAP (3.0 mg/l) + IAA (2.5 mg/l). The highest frequency of rooting (88.3%) was obtained on NAA (1.0 mg/l) and IBA (2.0 mg/l) with 21.83 ± 0.57 mean number of roots. The well-rooted healthy plantlets were acclimatized with a survival rate of 80%. Inter simple sequence repeat (ISSR) analysis revealed the genetic similarity of in vitro raised plants with the mother plant. Plant Tissue Cult. & Biotech. 30(2): 219-229, 2020 (December)

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Section: Resultsmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 99%

High Efficiency In vitro Regeneration and Genetic Stability of Corallocarpus epigaeus - An Endangered Medicinal Plant

Veera

Chirumamilla²,

Taduri

2020

Plant Tissue Cult. & Biotech.

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“…DNA based molecular markers emerged as the most suitable genetic tools to determine the genetic variation among regenerated plants 6 . Molecular markers such as randomly amplified polymorphic DNA (RAPD) 7 , amplified fragment length polymorphism (AFLP) 8 , simple sequence repeat (SSR) 9 and inter simple sequence repeats (ISSR) 10,11 are used for determining the genetic fidelity of micro propagated plants. Cost-effectiveness is a major checkpoint in micropropagation.…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic fidelity of lacy tree philodendron (Philodendron bipinnatifidum Schott ex Endl.) micro propagated plants

Alwahibi

Alawaadh

Dewir

et al. 2022

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Tissue culture is a potent means for producing clonally propagated plants. However, genetically identical regenerants are of great concern due to their economic consequences. Therefore, polymerase chain reaction (PCR)-based molecular markers are employed to detect somaclonal variations. In this study, the genetic fidelity of in vitro raised Philodendron bipinnatifidum clones, and their mother plant was tested using 11 randomly amplified polymorphic DNA (RAPD) markers. The RAPD decamers produced 92 amplicons with 8.4 bands ranging from 260-5000 bp. The bands varied from one to fifteen for primer 8 and primer 7, respectively. The genetic similarity between the micro propagated plantlets and mother plant of P. bipinnatifidum was nearly 100%, assuring uniformity and true-to-type regenerated plantlets for this commercially crucial ornamental plant.

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“…Table 5 below lists 12 plants (28%) that have been reported to be endangered and 31 tuberous anthelmintic plants are not endangered. Other endangered tuberous plants are Corallocarpus epigaeus(Vemula et al 2020), , , etc. Other anthelmintic plants that have been reported to be endangered areIIek khasiana(Lalnunfela et al 2020), Picrorhiza kurroa(Mehta et al 2021), Potentilla fulgens(Kumar et al 2020), Embelia ribes(Choudhary et al 2021b).…”

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confidence: 99%

Tuberous plants with active compounds against helminths in livestock: A systematic review

Mpofu

Mugumbate²,

Gomo³

et al. 2022

Ethnobot. Res. App.

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Background: The rise in drug resistance to helminthiases is posing a serious challenge to conventional techniques of controlling parasitic illnesses in livestock. Using less conventional approaches such as plant extracts can improve the situation. This research aims to explore the emerging role of tuberous plants in developing anthelmintics.Methods: From October 2020 to July 2022, a comprehensive literature search was conducted using the search engines Google Scholar, NISCAIR Online Periodicals Repository, NCBI, Taylor and Francis Online, Wiley Online Libraries, Science Direct, ResearchGate, and Springer Link. The evaluation included only tuberous plants with anthelmintic properties. Qualitative-quantitative analysis techniques were used to analyse the data collected.Results: Forty-eight ethnobotanical investigations recorded plants with tuber portions that were utilized to combat helminths. There were 43 plants identified, divided into 24 families. Seven plants were found to be the most culturally important in the management of helminths. The common phytochemical classes were phytosterols, tannins, alkaloids, saponins, essential oils, flavonoids, and terpenoids. Twenty-six of these tuberous plants have been tested for their anthelmintic effect against trematodes, cestodes, nematodes, protozoa, coccidian, and eoacanthacephala. Thirty-two plants have been reported to exhibit some toxicity effects. Twelve tuberous plants have been indicated to be endangered.Conclusions: Dioscorea deltoidea, Dioscorea bulbifera, Dioscorea alata, Gloriosa superba, Curcuma longa, Dioscorea pentaphylla, and Cyperus rotundus were the most culturally important plants for controlling helminths.These plants are mostly found in India and Nepal. The review gave more insights into the therapeutic safety of the popular folklore drugs and provided grounds for an assessment of possible measures to be introduced before conducting clinical studies. The article provides public and Government recognition of endangered plants species and spurs conservation efforts toward saving both plants and folk medicinal knowledge. There is a need to investigate other tuberous plants to identify unique compounds that are active against helminths to develop more robust anthelmintic drugs and reduce anthelmintic resistance levels.

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In vitro high frequency multiplication and assessment of genetic fidelity of Corallocarpus epigaeus: an endangered medicinal plant

Cited by 18 publications

References 40 publications

High Efficiency In vitro Regeneration and Genetic Stability of Corallocarpus epigaeus - An Endangered Medicinal Plant

High Efficiency In vitro Regeneration and Genetic Stability of Corallocarpus epigaeus - An Endangered Medicinal Plant

Assessment of genetic fidelity of lacy tree philodendron (Philodendron bipinnatifidum Schott ex Endl.) micro propagated plants

Tuberous plants with active compounds against helminths in livestock: A systematic review

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