In Vitro High-Throughput Toxicological Assessment of Nanoplastics

Tolardo, Valentina; Magrì, Davide; Fumagalli, Francesco; Cassano, Domenico; Athanassiou, Athanassia; Fragouli, Despina; Gioria, Sabrina

doi:10.3390/nano12121947

Cited by 11 publications

(2 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although in some studies, higher levels of DNA damage have been recorded in cells treated with NMPPs, others have found no correlation between NMPP exposure and genotoxic damage [17,39,46], confirming our findings. The same is true for the absence of major cytotoxic effects for particles of different types, origins, sizes, and concentrations [47][48][49]. Yet, cell membrane alterations could result from the internalization of the NMPP and direct physical contact between the membrane and NMPPs.…”

Section: Discussionmentioning

confidence: 94%

Optimized High-Content Imaging Screening Quantifying Micronuclei Formation in Polymer-Treated HaCaT Keratinocytes

et al. 2022

View full text Add to dashboard Cite

Research on nano- and micro-plastic particles (NMPPs) suggests their potential threat to human health. Some studies have even suggested genotoxic effects of NMPP exposure, such as micronuclei (MN) formation, while others found the opposite. To clarify the ability of NMPP to induce MN formation, we used non-malignant HaCaT keratinocytes and exposed these to a variety of polystyrene (PS) and poly methyl methacrylate (PMMA) particle types at different concentrations and three different sizes. Investigations were performed following acute (one day) and chronic exposure (five weeks) against cytotoxic (amino-modified NMPPs) and genotoxic (methyl methanesulfonate, MMS) positive controls. An optimized high-content imaging workflow was established strictly according to OECD guidelines for analysis. Algorithm-based object segmentation and MN identification led to computer-driven, unsupervised quantitative image analysis results on MN frequencies among the different conditions and thousands of cells per condition. This could only be realized using accutase, allowing for partial cell detachment for optimal identification of bi-nucleated cells. Cytotoxic amino-modified particles were not genotoxic; MMS was both. During acute and long-term studies, PS and PMMA particles were neither toxic nor increased MN formation, except for 1000 nm PS particles at the highest concentration of unphysiological 100 µg/mL. Interestingly, ROS formation was significantly decreased in this condition. Hence, most non-charged polymer particles were neither toxic nor genotoxic, while aminated particles were toxic but not genotoxic. Altogether, we present an optimized quantitative imaging workflow applied to a timely research question in environmental toxicity.

show abstract

Section: Discussionmentioning

confidence: 94%

Optimized High-Content Imaging Screening Quantifying Micronuclei Formation in Polymer-Treated HaCaT Keratinocytes

et al. 2022

View full text Add to dashboard Cite

show abstract

“…To assess the potential hepatotoxicity of MPs or NPs, in vitro studies have been performed mainly using hepatocarcinoma cell lines (such as HepG2 and HepaRG cells) as human hepatic cell models. 30–34 Overall, the data indicate that, at doses in the range of 0–100 μg mL −1 , plastic particles induce cell viability reduction, oxidative stress, inflammation, or the formation of binucleated cells (possibly indicative of impaired cytokinesis). However, poor information is currently available on the effects exerted by NPs on hepatocyte-specific functions ( e.g.…”

Section: Introductionmentioning

confidence: 92%