In vitro host range of the Hz-1 nonoccluded virus in insect cell lines

McIntosh, Arthur H.; Grasela, James J.; Ignoffo, Carlo M.

doi:10.1007/s11626-007-9032-6

Cited by 5 publications

(6 citation statements)

References 36 publications

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“…The permissive cell line BCIRL-HzAM1 produced a titer of 3.3ϫ10 5 TCID 50 /ml following challenge with HzSNPV. The Hz-1 virus which has a very wide in vitro host range (McIntosh et al, 2007) produced a titer of 3.7ϫ10 6 TCID 50 /ml in BCIRL-DP-AM/JG as contrasted with a 2 log cycle higher titer of 4.3ϫ10 8 TCID 50 /ml in the permissive cell line BCIRLHzAM1.…”

Section: Resultsmentioning

confidence: 97%

“…The BCIRL-DP-AM/JG was susceptible to 4 baculoviruses used in this study but was refractile to a 5th, the HzSNPV. The monarch butterfly cell line was also susceptible to the Hz-1 non-occluded virus which does not produce OB but results in the degeneration of inoculated cells (McIntosh et al, 2007). AcMNPV-HPP replicated to a titer of 1.9ϫ10 7 TCID 50 /ml in BCIRL-DP-AM/JG as compared with 3.2ϫ10 7 TCID 50 /ml in the permissive cell line BCIRL-HsAM1.…”

Section: Resultsmentioning

confidence: 99%

“…The inoculated cells were placed on a rocker platform for 2 h, the inoculum removed and the cells washed three times with HBSS and 5 ml of growth medium added to each flask and incubated at 28°C for 7 days. At the end of this period the growth medium from the inoculated flasks was removed and centrifuged in a TJ-6 natant fluids were removed and TCID 50 assays performed on each sample as previously described (McIntosh et al, 2007). The pellet from each sample was re-suspended in 5 ml of UP-water, sonicated to disrupt cells to release the occlusion bodies (OB) where applicable and enumerated in a hemacytometer.…”

Section: Susceptibility Of Bcirl-dp-am/jg and Selected Lepidopteran Cmentioning

confidence: 99%

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Establishment of a monarch butterfly (Danaus plexippus, Lepidoptera: Danaidae) cell line and its susceptibility to insect viruses

McIntosh

Grasela

2009

Appl. Entomol. Zool.

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A cell line from the monarch butterfly Danaus plexippus designated BCIRL-DP-AM/JG was established from adult ovaries. The cell line consisted mainly of round cells and took a prolonged period of time in the growth medium ExCell 401 containing 10% fetal bovine serum and antibiotics before it could be subcultured on a regular basis. The cell line had a population doubling time of 32 h and was susceptible to four baculoviruses (MNPV) but was refractile to a fifth baculovirus, a single nucleopolyhedrovirus (HzSNPV). PxMNPV was the most infectious of the MNPV for BCIRL-DP-AM/JG cell line of D. plexippus producing a titer of 5.9ϫ107 TCID 50 /ml. The non-occluded Hz-1 virus was also infectious for the D. plexippus cell line. The occlusion bodies produced by the BCIRL-DP-AM/JG cell line were infectious for 24 h old Trichoplusia ni larvae and gave LC 50 values equivalent to or better than the LC 50 for OB produced in the other susceptible cell lines by the MNPV under study. The identity of the monarch butterfly cell line was established by DAF-PCR.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Susceptibility Of Bcirl-dp-am/jg and Selected Lepidopteran Cmentioning

confidence: 99%

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Establishment of a monarch butterfly (Danaus plexippus, Lepidoptera: Danaidae) cell line and its susceptibility to insect viruses

McIntosh

Grasela

2009

Appl. Entomol. Zool.

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“…(47,48). The HzNV-1 virus has a relatively broad host range and has been reported to productively and latently infect many insect cell lines (4,14,24,31,34,35,40). Previously, we found that more than 100 transcripts are produced during productive HzNV-1 virus infection (6); during this phase of infection, a gene located on the HzNV-1 HindIII-I fragment (hhi1) was found to generate an abundant 6.2-kb transcript within 0.5 h postinfection (hpi) (50,51).…”

mentioning

confidence: 99%

Heliothis zea Nudivirus 1 Gene hhi1 Induces Apoptosis Which Is Blocked by the Hz-iap2 Gene and a Noncoding Gene, pag1

Liu

et al. 2011

J Virol

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Heliothis zea nudivirus 1 (HzNV-1 or Hz-1 virus), previously regarded as a nonoccluded baculovirus, recently has been placed in the Nudivirus genus. This virus generates HzNV-1 HindIII-I 1 (hhi1) and many other transcripts during productive viral infection; during latent viral infection, however, persistency-associated gene 1 (pag1) is the only gene expressed. In this report, we used transient expression assays to show that hhi1 can trigger strong apoptosis in transfected cells, which can be blocked, at least partially, by the inhibitor of apoptosis genes Autographa californica iap2 (Ac-iap2) and H. zea iap2 (Hz-iap2). In addition to these two genes, unexpectedly, pag1, which encodes a noncoding RNA with no detectable protein product, was found to efficiently suppress hhi1-induced apoptosis. The assay of pro-Sf-caspase-1 processing by hhi1 transfection did not detect the small P12 subunit at any of the time intervals tested, suggesting that hhi1 of HzNV-1 induces apoptosis through alternative caspase pathways.

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“…This virus has been reported to infect many insect cell lines including Trichoplusia ni (TN-368), Spodoptera frugiperda (IPLB-SF-21), H. zea (IPLB-1075), Mamestra brassicae, and Porthetria dispar (IPLB-65Z) (1,8,22,25) and to establish latent infections in most of these insect cell lines, which are permissive to this virus. HzNV-1 virus is an enveloped, rodshaped, nonoccluded virus with a circular double-stranded DNA genome of 228 kb, which is encased in a 414 Ϯ 30 nm nucleocapsid (1,3,4,11).…”

mentioning

confidence: 99%

The Early Gene hhi1 Reactivates Heliothis zea Nudivirus 1 in Latently Infected Cells

Lee

et al. 2010

J Virol

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Heliothis zea nudivirus 1 (HzNV-1), previously known as Hz-1 virus, is an insect virus able to establish both productive and latent infections in several lepidopteran insect cells. Here, we have cloned and characterized one of the HzNV-1 early genes, hhi1, which maps to the HindIII-I fragment of the viral genome. During the productive viral infection, a 6.2-kb hhi1 transcript was detectable as early as 0.5 h postinfection (hpi). The level of transcript reached a maximum at 2 hpi and gradually decreased after 4 hpi. The transcript was not detectable during the latent phase of viral infection. Upon cycloheximide treatment, much higher levels of hhi1 transcript were detected throughout the productive viral infection cycle, suggesting that newly synthesized proteins are not needed for the expression of hhi1. Nevertheless, viral coinfection can further stimulate the expression of transfected hhi1 promoter in a plasmid. Transient hhi1 expression in latently infected cells resulted in a significant increase in virus titer and viral DNA propagation, suggesting that hhi1 plays a critical role in viral reactivation. Additional experiments showed that six early genes, which possibly function in transcription or DNA replication, were activated in the latent cells upon hhi1 transfection. Among these six genes, orf90 and orf121 expression could be induced by hhi1 alone without the need for other viral genes. Our discovery should be useful for future mechanistic study of the switches of latent/productive HzNV-1 viral infections.

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In vitro host range of the Hz-1 nonoccluded virus in insect cell lines

Cited by 5 publications

References 36 publications

Establishment of a monarch butterfly (Danaus plexippus, Lepidoptera: Danaidae) cell line and its susceptibility to insect viruses

Establishment of a monarch butterfly (Danaus plexippus, Lepidoptera: Danaidae) cell line and its susceptibility to insect viruses

Heliothis zea Nudivirus 1 Gene hhi1 Induces Apoptosis Which Is Blocked by the Hz-iap2 Gene and a Noncoding Gene, pag1

The Early Gene hhi1 Reactivates Heliothis zea Nudivirus 1 in Latently Infected Cells

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