In Vitro Human Umbilical Vein Endothelial Cells Response to Ionic Dissolution Products from Lithium-Containing 45S5 Bioactive Glass

Durand, Luis; Vargas, Gabriela E.; Vera-Mesones, Rosa; Baldi, Alberto; Zago, M. Paola; Fanovich, María Alejandra; Boccaccını, Aldo R.; Gorustovich, Alejandro A.

doi:10.3390/ma10070740

Cited by 36 publications

(24 citation statements)

References 69 publications

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“…Moreover, these biomaterials have exhibited antibacterial properties against methicillin resistant Staphylococcus aureus . On the other hand, dissolution products from 45S5 BAG doped with 5 wt% Li have exhibited a pro‐angiogenic activity on HUVECs involving the canonical Wnt/β‐catenin pathway . However, bioactivity is slightly impacted probably due to the substitution of either Li or Sr for Ca …”

Section: Optimization Of Bioactive Glass Hybridsmentioning

confidence: 99%

Optimized Bioactive Glass: the Quest for the Bony Graft

Granel

Bossard

Nucke

et al. 2019

Adv Healthcare Materials

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Technological advances have provided surgeons with a wide range of biomaterials. Yet improvements are still to be made, especially for large bone defect treatment. Biomaterial scaffolds represent a promising alternative to autologous bone grafts but in spite of the numerous studies carried out on this subject, no biomaterial scaffold is yet completely satisfying. Bioactive glass (BAG) presents many qualifying characteristics but they are brittle and their combination with a plastic polymer appears essential to overcome this drawback. Recent advances have allowed the synthesis of organic–inorganic hybrid scaffolds combining the osteogenic properties of BAG and the plastic characteristics of polymers. Such biomaterials can now be obtained at room temperature allowing organic doping of the glass/polymer network for a homogeneous delivery of the doping agent. Despite these new avenues, further studies are required to highlight the biological properties of these materials and particularly their behavior once implanted in vivo. This review focuses on BAG with a particular interest in their combination with polymers to form organic–inorganic hybrids for the design of innovative graft strategies.

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Section: Optimization Of Bioactive Glass Hybridsmentioning

confidence: 99%

Optimized Bioactive Glass: the Quest for the Bony Graft

Granel

Bossard

Nucke

et al. 2019

Adv Healthcare Materials

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“…(14,68,69) Among Si, N, and P, Si is the most studied and supported in enhancing angiogenesis. (11,37,39,70,71) As shown in Table 2, EDS analysis shows that the difference between SiONx, SiONPx1, and SiONPx2 (O: 14.2 at %) groups is mainly represented by Si, O, and N at %, as P represents <1 at % of surface composition. However, the addition of P and the different N 2 O flow rate between SiONPx1 and SiONPx2 (Table 1) played a role in the elemental surface composition of these two coatings.…”

Section: Discussionmentioning

confidence: 96%

Silicon Oxynitrophosphide Nanoscale Coating Enhances Antioxidant Marker‐Induced Angiogenesis During in vivo Cranial Bone‐Defect Healing

et al. 2021

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Critical sized bone defects are challenging to heal due to the sudden and large volume of lost bone. Fixative plates are often used to stabilize defects, yet oxidative stress and delayed angiogenesis are contributing factors to poor biocompatibility and delayed bone healing. This study tests the angiogenic and antioxidant properties of amorphous silicon oxynitrophosphide (SiONPx) nanoscale coating material on endothelial cells to regenerate vascular tissue in vitro and in bone defects. In vitro studies evaluate the effect of SiONx and two different SiONPx compositions on human endothelial cells exposed to reactive oxygen species (e.g., H2O2) that simulates oxidative stress conditions. In vivo studies using adult male Sprague Dawley rats (~450 g) were performed to compare a bare plate, SiONPx-coated implant plate, and a sham control group using a rat standard sized calvarial defect. Results from this study showed that plates coated with SiONPx significantly reduced cell death, enhanced vascular tubule formation and matrix deposition by upregulating angiogenic and antioxidant expression (e.g. VEGFA, angiopoetin-1, SOD-1, NRF-2 and Cat-1). Moreover, endothelial cell markers (CD31) demonstrate a significant tubular structure on the SiONPx coating group compared to an empty and uncoated plate group. This reveals that atomic doping of phosphate into the nanoscale coating of SiONx produced markedly elevated levels of antioxidant and angiogenic markers that enhance vascular tissue regeneration. This study demonstrates that SiONPx or SiONx nanoscale coated materials enhance antioxidant expression, angiogenic marker expression, and reduce ROS levels needed for accelerating vascular tissue regeneration. These results further suggest that SiONPx nanoscale coating could be a promising candidate for titanium plate for rapid and enhanced cranial bone defect healing.

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“…There are several growth factors involved in blood vessel formation and stabilization. Among them, VEGF, the angiopoietin (Ang) family of growth factors (e.g., Ang 2), PDGF, FGF, epidermal growth factor (EGF), transforming growth factor-β (TGF-β), IL-8, and hypoxiainducible factor 1 (HIF), have been often described in scaffold vascularization studies in tissue engineering (Blatt et al, 2020;Chandra & Atala, 2019;Chen et al, 2017;Cheung et al, 2015;Haro Durand et al, 2017;Heller et al, 2020Heller et al, , 2016Kniebs et al, 2020;Perez-Amodio et al, 2011;Samal et al, 2015;Sarker et al, 2015;Xiao et al, 2015;Zhang et al, 2012). The quantification of these proangiogenic markers is commonly performed by enzyme-linked immunosorbent assay (ELISA), however, other methods such as IHC, IF, flow cytometry, transcription analysis (PCR) (Shi et al, 2014) and proteomics (e.g., Western blot) (Unterleuthner et al, 2017) have also been used.…”

Section: Quantification Of Pro-angiogenic Growth Factorsmentioning

confidence: 99%

Vascularization strategies in tissue engineering approaches for soft tissue repair

Masson-Meyers

Tayebi

2021

J Tissue Eng Regen Med

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Insufficient vascularization during tissue repair is often associated with poor clinical outcomes. This is a concern especially when patients have critical-sized injuries, where the size of the defect restricts vascularity, or even in small defects that have to be treated under special conditions, such as after radiation therapy (relevant to tumor resection) that hinders vascularity. In fact, poor vascularization is one of the major obstacles for clinical application of tissue engineering methods in soft tissue repair. As a key issue, lack of graft integration, caused by inadequate vascularization after implantation, can lead to graft failure. Moreover, poor vascularization compromises the viability of cells seeded in deep portions of scaffolds/graft materials, due to hypoxia and insufficient nutrient supply. In this article we aim to review vascularization strategies employed in tissue engineering techniques to repair soft tissues. For this purpose, we start by providing a brief overview of the main events during the physiological wound healing process in soft tissues. Then, we discuss how tissue repair can be achieved through tissue engineering, and considerations with regards to the choice of scaffold materials, culture conditions, and vascularization techniques. Next, we highlight the importance of vascularization, along with strategies and methods of prevascularization of soft tissue equivalents, particularly cellbased prevascularization. Lastly, we present a summary of commonly used in vitro methods during the vascularization of tissue-engineered soft tissue constructs.

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In Vitro Human Umbilical Vein Endothelial Cells Response to Ionic Dissolution Products from Lithium-Containing 45S5 Bioactive Glass

Cited by 36 publications

References 69 publications

Optimized Bioactive Glass: the Quest for the Bony Graft

Optimized Bioactive Glass: the Quest for the Bony Graft

Silicon Oxynitrophosphide Nanoscale Coating Enhances Antioxidant Marker‐Induced Angiogenesis During in vivo Cranial Bone‐Defect Healing

Vascularization strategies in tissue engineering approaches for soft tissue repair

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