In vitro hydroxyapatite adsorbed salivary proteins

Vitorino, Rui; Lobo, Maria João C.; Duarte, José Alberto; Ferrer‐Correia, A. J.; Tomer, Kenneth B.; Dubin, Joshua R.; Domingues, Pedro; Amado, Francisco

doi:10.1016/j.bbrc.2004.05.169

Cited by 34 publications

(26 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…14 Bicarbonate ions can neutralize lactic and acetic acids produced by plaque bacteria and reduce demineralization. CAVI can adsorbs to the crystals of hydroxyapatite of enamel; 15 it is present in the enamel pellicle 16 and in dental biofilm. 17 Salivary buffer capacity seems to be a determinant factor on dental erosion, which is becoming a major dental problem in both children and adults.…”

Section: Introductionmentioning

confidence: 99%

Association of polymorphisms in the carbonic anhydrase 6 gene with salivary buffer capacity, dental plaque pH, and caries index in children aged 7–9 years

et al. 2009

View full text Add to dashboard Cite

Carbonic anhydrase VI is a secreted enzyme that catalyzes the hydration of carbon hydroxide in saliva and other body fluids. This enzyme has been implicated in taste and gastrointestinal dysfunctions, tooth erosion, and caries. The purpose of this study was to analyze the allele and genotype distribution of three polymorphisms in the coding sequences of (CA6) gene and check for possible associations with salivary buffer capacity, number of decayed, missing, and filled teeth in deciduous and permanent teeth (dmft/ DMFT, Decayed/Missing/Filled Teeth), plaque index (PI), and the plaque pH variation (DpH) in children aged 7-9 years. Two hundred and forty-five children from both genders, residents in area with fluoridated water (Piracicaba, São Paulo, Brazil) were divided into two groups: caries free and with caries. The clinical examinations were conducted by a single previously calibrated examiner (k ¼ 0.91) in an outdoor setting using a mirror and a probe, according to WHO criteria index (dmft/DMFT). Approximately 2 h after the first daily meal, the buffer capacity (BC) and the plaque pH were analyzed by means of a pH meter and an ion selective electrode. Plaque pH was measured immediately and 5 min after a mouth rinse with a 10% sucrose solution. The data were submitted to w 2 , Student's, and Mann-Whitney tests (a ¼ 0.05). The PI and DpH of the upper and lower teeth were significantly higher in the carious group than control (Po0.05). There was no difference between the groups in relation to BC. There was no association between the alleles and genotypes distributions for polymorphisms in the CA6 gene exons 2 and 3 and caries experience (P40.05). There was a positive association between buffer capacity and the rs2274327 (C/T) polymorphism. The allele T and genotype TT were significantly less frequent in individuals with the highest buffer capacity (P ¼ 0.023 and 0.045, respectively). This finding encourages future studies relating CA6 gene polymorphisms and their association with malfunctions, such as taste and gastrointestinal alterations, or the differential effect of chemical modulators on the protein products originated from the distinct genotypes of the CA6 gene.

show abstract

Section: Introductionmentioning

confidence: 99%

Association of polymorphisms in the carbonic anhydrase 6 gene with salivary buffer capacity, dental plaque pH, and caries index in children aged 7–9 years

et al. 2009

View full text Add to dashboard Cite

show abstract

“…The predicted pockets are consistent by different methods at the same fragments, and most high scorning pockets exist in the FN-III 10 fragment and the hinge areas of different modules of FN. The binding sites and interaction free energies between the FN-III 7-10 and HAP were further examined using the tool suite of AutoDock 4 [19]. Both the ligand and the receptor were treated as rigid and we only explored the six degrees of translational and rotational freedom, hence excluding any kind of flexibility.…”

Section: Identification Of Potential Hap-binding Sites On Fn-iii 7-10mentioning

confidence: 99%

“…Numerous experimental methods have been developed to investigate the protein adsorption with HA, and researchers have studied the adsorption of proteins on the surface of biomaterials by the methods such as atomic force microscopy (AFM) [16], flow microcalorimetry (FMC) [17], solid state NMR [18], 2D electrophoresis [19], and steered molecular dynamics (SMD) simulations [20]. In this work, the interaction mechanism of FN-III [7][8][9][10] , which contains the RGD loop, with HAP molecules was investigated systematically by using a molecular docking strategy.…”

Section: Introductionmentioning

confidence: 99%

Molecular Docking Characterization of a Four-Domain Segment of Human Fibronectin Encompassing the RGD Loop with Hydroxyapatite

et al. 2013

View full text Add to dashboard Cite

Fibronectin adsorption on biomaterial surfaces plays a key role in the biocompatibility of biomedical implants. In the current study, the adsorption behavior of the 7-10th type III modules of fibronectin ) in the presence of hydroxyapatite (HAP) was systematically investigated by using molecular docking approach. It was revealed that the FN-III 10 is the most important module among FN-III 7-10 in promoting fibronectin binding to HAP by optimizing the interaction energy; the arginine residues were observed to directly interact with the hydroxyl group of HAP through electrostatic forces and hydrogen bonding. Moreover, it was found that the HAP-binding sites on FN-III 10 are mainly located at the RGD loop region, which does not affect the interaction between the fibronectin protein and its cognate receptors on the cell surface.

show abstract

“…Using 2DE in combination with MS, whole salivary proteins were separated and 5-9 proteins were identified. [22][23][24][25][26][27][28] 2DE technologies have many advantages, they provide information about protein modifications and proteolysis and produce quantitative values. Recently, advances in MS technologies and high resolution nanoflow LC have produced MS-based proteomics known as shotgun proteomics.…”

Section: Proteome Analysis Of Salivamentioning

confidence: 99%

The Current Status and Future Prospects of the Salivary Proteome

Yamada

Yuji

Suzuki

2009

JOURNAL OF HEALTH SCIENCE

View full text Add to dashboard Cite

Saliva performs many biological functions that are instrumental in maintaining oral health. Digestive enzymes, such as alpha-amylase and antimicrobial proteins, such as lysozyme and cystatin, are present at high concentrations in human saliva. It is a readily available atraumatic body fluid, useful in the diagnosis of disease, aging and oral health. Large scale analysis of salivary proteins is important for biomarker discovery. However, the abundance of some salivary proteins and contamination with proteins from food and mouth bacteria obscures identification of medium and low copy salivary proteins. In this review, we summarize current knowledge of the salivary proteome using mass spectrometry based technologies.

show abstract

In vitro hydroxyapatite adsorbed salivary proteins

Cited by 34 publications

References 20 publications

Association of polymorphisms in the carbonic anhydrase 6 gene with salivary buffer capacity, dental plaque pH, and caries index in children aged 7–9 years

Association of polymorphisms in the carbonic anhydrase 6 gene with salivary buffer capacity, dental plaque pH, and caries index in children aged 7–9 years

Molecular Docking Characterization of a Four-Domain Segment of Human Fibronectin Encompassing the RGD Loop with Hydroxyapatite

The Current Status and Future Prospects of the Salivary Proteome

Contact Info

Product

Resources

About