2018
DOI: 10.1016/j.bbamcr.2018.01.012
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In vitro identification of mitochondrial oxidative stress production by time-resolved fluorescence imaging of glioma cells

Abstract: Oxidative phosphorylation and glycolysis are important features, by which cells could bypass oxidative stress. The level of oxidative stress, and the ability of cells to promote oxidative phosphorylation or glycolysis, significantly determined proliferation or cell demise. In the present work, we have employed selective mitochondrial probe MitoTracker™ Orange CMTM/Ros (MTO) to estimate the level of oxidative stress in cancer cells at different stressed conditions. MTO is partially sensitive to decrease of mito… Show more

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Cited by 23 publications
(30 citation statements)
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“…Microscopy with Mitotracker orange (stains mitochondria in live cells) was performed with control, menadione-treated (10 µM) and picein-treated (25 µM) samples after menadione exposure. The live cell imaging of SH-SY5Y cells after menadione exposure showed low emission of Mitotracker fluorescence, could be indicating an overproduction of ROS, as also reported by Tomkova et al [45]. Moreover, the qualitative observations from Figure 7 clearly reveal that the menadione-exposed cells were morphologically distinct with structural abnormalities, and such deviations could be interpreted as mitochondrial dysfunction, as has been reported by other researchers [46,47].…”
Section: Live Cell Imagingsupporting
confidence: 83%
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“…Microscopy with Mitotracker orange (stains mitochondria in live cells) was performed with control, menadione-treated (10 µM) and picein-treated (25 µM) samples after menadione exposure. The live cell imaging of SH-SY5Y cells after menadione exposure showed low emission of Mitotracker fluorescence, could be indicating an overproduction of ROS, as also reported by Tomkova et al [45]. Moreover, the qualitative observations from Figure 7 clearly reveal that the menadione-exposed cells were morphologically distinct with structural abnormalities, and such deviations could be interpreted as mitochondrial dysfunction, as has been reported by other researchers [46,47].…”
Section: Live Cell Imagingsupporting
confidence: 83%
“…MitoSOX red, a novel fluorogenic probe, can bind to the nuclear DNA and therefore identify superoxide in the live cells of mitochondria [64]. In addition to this, the selective mitochondrial probe Mitotracker orange was employed in this study, which is highly receptive to mitochondrial membrane potential and considered a sensitive indicator for the measurement of oxidative stress in mitochondria [45]. The accumulation of Mitotracker in cells mainly depends on the mitochondrial membrane potential [68] because it disseminates throughout the plasma membrane and accumulates in live mitochondria.…”
Section: Discussionmentioning
confidence: 99%
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“…Briefly, the OCR was measured by the sequential addition of oligomycin (an ATP synthase inhibitor, 1 μM), phenylhydrazone (FCCP, a mitochondrial respiration uncoupler, 0.5 μM), and rotenone (a complete respiratory inhibitor, 2 μM). This protocol is normally used for the determination of basal OCR, ATP‐linked respiration, and maximal respiration (Tomkova, Misuth, Lenkavska, Miskovsky, & Huntosova, ). After the measurement of mitochondrial respiratory capacity, the cells were lysed, and the total protein was quantified using the BCA protein assay.…”
Section: Methodsmentioning
confidence: 99%
“…Myelin is not an inert molecule that simply coats the axon but rather a metabolically active structure: in fact, myelin and the neuronal axon must be regarded as a single metabolically combined functional unit. Oligodendrocyte creates myelin channels—located at the end of the axon—that allow for lipid macromolecules to be transported and moved [20].…”
Section: Introductionmentioning
confidence: 99%