In vitro immune response of human peripheral blood cells to vitamins C and E

Bergman, Michael; Salman, Hertzel; Djaldetti, M; Fish, Lev; Punsky, Igor; Bessler, Hanna

doi:10.1016/j.jnutbio.2003.10.001

Cited by 33 publications

(31 citation statements)

References 26 publications

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“…These findings are in agreement with a previous in vitro study performed on human polymorphonuclear cells, that pointed out that incubation of neutrophils and monocytes with vitamin C caused a significant increase in the cellular phagocytic capacity, but did not affect the number of cells capable for phagocytosis, whereas incubation with vitamin E did not modify phagocytic capacity (Bergman et al, 2004). These results, although observed in vitro and in other cells, and our findings may be of importance to show that antioxidant vitamins exert a protective effect to oxidative stress on human cells, but also that the intense exercise promotes mechanisms to accumulate antioxidant vitamins into cells sensitive to effects of reactive oxygen species (ROS).…”

Section: Discussionsupporting

confidence: 93%

Differential response of plasma and immune cell's vitamin E levels to physical activity and antioxidant vitamin supplementation

et al. 2005

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Objective: To assess the differential response of plasma, lymphocyte and neutrophil vitamin E levels to high-intensity physical activity and to vitamin C and E supplementation. Subjects: In all, 14 male trained amateur runners (32-36 y old) were randomly divided in two groups (supplemented and placebo), and participated in a half marathon race. The subjects did not take any other supplements than the ones provided for this study. Intervention: Vitamin C (152 mg/day) and E (50 mg/day) supplementation was administrated to athletes for a month, using a new almond-based isotonic and energetic beverage (supplemented group). The usual dietary habits of participants were assessed using a self-reported 7-day 24-h recall before the day of the study. To avoid the beverage influence, nonenriched vitamin C and E almond-based isotonic and energetic beverage was given to the placebo group. After 1 month, subjects participated in a half marathon race (21 km run). Vitamin E concentration was determined in plasma, neutrophils and lymphocytes before and immediately after the race, and 3 h after finishing the race. Results: Daily energy intake and caloric profile of supplemented and placebo group were not different except for vitamin C and E supplementation. Vitamin supplementation and exercise had no effect on vitamins E levels in plasma. The exercise significantly (Po0.05) increased the lymphocyte vitamin E concentration both in the placebo ( þ 119%) and supplemented groups ( þ 128%), and neutrophil vitamin E content in the supplemented group ( þ 88%). These levels remained significantly (Po0.05) high after the short recovery. After exercise, vitamin E levels in lymphocytes and neutrophils of supplemented subjects were practically twice the levels before exercise, whereas neutrophil vitamin E content of the placebo group was close to those in plasma. Conclusion: After endurance exercise, lymphocytes increased their vitamin E content in the supplemented and placebo subjects whereas this trend in neutrophils was just observed in the supplemented group. The determination of vitamin E content in lymphocytes and neutrophils after exercise is a useful tool to assess the functional status of vitamin E.

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Section: Discussionsupporting

confidence: 93%

Differential response of plasma and immune cell's vitamin E levels to physical activity and antioxidant vitamin supplementation

et al. 2005

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“…It is notable that other lipid-soluble vitamins and compounds such as vit. E and lycopene exert immunomodulatory effects as we have shown previously (17,18). Studies have shown that CoQ10 exerts a synergistic effect when added to anti-cancer drugs.…”

Section: Discussionmentioning

confidence: 64%

Coenzyme Q10 Decreases TNF-α and IL-2 Secretion by Human Peripheral Blood Mononuclear Cells

Bessler

Bergman

Blumberger

et al. 2010

J Nutr Sci Vitaminol

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SummaryThe beneficial effect of coenzyme Q10 (CoQ10) on human health occurs through various mechanisms including the possibility of immunomodulation. Therefore, the purpose of study was to examine the in vitro effect of CoQ10 on cytokine production and superoxide anion generation by human peripheral blood mononuclear cells (PBMC). 2 ϫ 10 6 /mL PBMC obtained from 19 volunteers were incubated for 24 h without or with 0.6, 1.25, 2.5 and 5.0 M of CoQ10. The production of the following cytokines were examined: IL-1 ␤ , IL-1ra, IL-6, IL-10, IL-2 and IFN ␥ . Superoxide anion production was examined by incubation of 4 ϫ 10 6 /mL cells with CoQ10 and 2 ϫ 10 Ϫ 3 m M phorbol merystate acetate (PMA) for 60 min. The production of the proinflammatory cytokines IL-1 ␤ , IL-6 and IFN ␥ and that of the anti-inflammatory cytokines IL-1ra and IL-10 by PBMC was not affected by CoQ10, whereas TNF ␣ secretion was significantly decreased when the cells were incubated with 0.6 and 1.25 M of CoQ10. On the other hand, increasing doses of CoQ10 caused mild, but statistically significant inhibition of IL-2 secretion. The generation of superoxide anion by PBMC did not differ significantly between cells incubated with or without CoQ10 at concentrations between 0.3 and 5.0 M . The results suggest that CoQ10 exerts a certain effect on cytokine production by PBMC related to its capacity to modulate human immune function.

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“…Other research found that chemotactic activity of cells from cultures grown in a medium with vitamin A or both -tocopherol and vitamin A was substantially lower than that of neutrophils obtained from cultures grown in a medium supplemented with only -tocopherol. On the other hand, no significant influence of these antioxidants on phagocytic activity was observed compared to cells incubated in a supplement-free medium (Bergman et al 2004). …”

Section: Discussionmentioning

confidence: 92%

In vitro Effect of α-tocopherol and Ascorbic Acid Supplementation on Immunological Indicators in Bovine Leukocytes following Transportation

et al. 2009

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The aim of the present study was to evaluate the effect of two different antioxidants, -tocopherol and ascorbic acid, on the chemotactic activity and susceptibility to Mannheimia haemolytica leukotoxin (Lkt) of white blood cells obtained from calves exposed to transport in vitro.Both antioxidants used were found to affect the chemotactic activity of white blood cells. Supplementation of -tocopherol at a concentration of 0.2 mg/ml and 0.5 mg/ml induced significant (P  0.05) increase of chemotactic activity in white blood cells compared to control. In case of ascorbic acid supplementation the highest chemotactic activity was observed at a concentration of 0.5 mg/ml. The results obtained in microtitration (MTT) assay also suggest that high concentrations of -tocopherol (0.5 mg/ml) play a role in protecting bovine leukocytes against the cytotoxic effect of Lkt, the susceptibility of the leukocytes on M. haemolytica Lkt was lower in comparison to control about 6%.The antioxidants used in this study have been found to have a protective effect on cells participating in the immune response to M. haemolytica. This particularly applies to high concentrations of -tocopherol. The clinical aspect of these results may lie in protection of leukocytes exposed to leukotoxin of M. haemolytica.It should be emphasized that the results provide only partial information on the effect of the antioxidants studied on cells participating in an organism's defence mechanisms. For an evaluation that would enable proper prevention of bovine respiratory diseases, research should be continued and expanded to include analysis of cellular structures.

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In vitro immune response of human peripheral blood cells to vitamins C and E

Cited by 33 publications

References 26 publications

Differential response of plasma and immune cell's vitamin E levels to physical activity and antioxidant vitamin supplementation

Differential response of plasma and immune cell's vitamin E levels to physical activity and antioxidant vitamin supplementation

Coenzyme Q10 Decreases TNF-α and IL-2 Secretion by Human Peripheral Blood Mononuclear Cells

In vitro Effect of α-tocopherol and Ascorbic Acid Supplementation on Immunological Indicators in Bovine Leukocytes following Transportation

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