In vitro immunomodulatory activities of peptides derived from Salmo salar NK-lysin and cathelicidin in fish cells

Acosta, Jannel; Roa, Francisco J.; González-Chavarría, Iván; Astuya, Allisson; Maura, Rafael; Montesino, Raquel; Muñoz, Carolina; Camacho, Frank; Saavedra, Paulina; Valenzuela, Ariel; Sánchez, Oliberto; Toledo, Jorge R.

doi:10.1016/j.fsi.2019.03.034

Cited by 66 publications

(35 citation statements)

References 58 publications

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“…Finally, it is worth noting that lncRNAs might participate in the immunopathogenesis of CanL, as they are critical regulators of gene transcription during immune response through regulating protein-protein interactions or via their ability to assemble with RNA and DNA ( 147 – 150 ). Accordingly, we found that changes in the expression of several lncRNAs were significantly associated with CanL, including an antimicrobial peptide NK-lysin-like ( LOC608395 ) and JUN , which play immunomodulatory roles ( 151 , 152 ). In particular, JUN could be a key immune regulator during L. infantum infection, since it is the second most differentially expressed lncRNA in the lymph nodes of dogs with CanL and regulates proinflammatory cytokines, chemokines and NO production ( 152 ), essential to achieve parasite control.…”

Section: Discussionmentioning

confidence: 99%

Modulation of Host Immune Response during Leishmania infantum Natural Infection: A Whole-Transcriptome Analysis of the Popliteal Lymph Nodes in Dogs

et al. 2022

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Leishmania infantum, the etiological agent of canine leishmaniosis (CanL) in Europe, was responsible of the largest outbreak of human leishmaniosis in Spain. The parasite infects and survives within myeloid lineage cells, causing a potentially fatal disease if left untreated. The only treatment option relies on chemotherapy, although immunotherapy strategies are being considered as novel approaches to prevent progression of the disease. To this aim, a deeper characterization of the molecular mechanisms behind the immunopathogenesis of leishmaniosis is necessary. Thus, we evaluated, for the first time, the host immune response during L. infantum infection through transcriptome sequencing of the popliteal lymph nodes aspirates of dogs with CanL. Differential expression and weighted gene co-expression network analyses were performed, resulting in the identification of 5,461 differentially expressed genes (DEGs) and four key modules in sick dogs, compared to controls. As expected, defense response was the highest enriched biological process in the DEGs, with six genes related to immune response against pathogens (CHI3L1, SLPI, ACOD1, CCL5, MPO, BPI) included among the ten most expressed genes; and two of the key co-expression modules were associated with regulation of immune response, which also positively correlated with clinical stage and blood monocyte concentration. In particular, sick dogs displayed significant changes in the expression of Th1, Th2, Th17 and Tr1 cytokines (e. g. TNF-α, IFN-γ, IL-21, IL-17, IL-15), markers of T cell and NK cell exhaustion (e. g. LAG3, CD244, Blimp-1, JUN), and B cell, monocyte and macrophage disrupted functionality (e. g. CD40LG, MAPK4, IL-1R, NLRP3, BCMA). In addition, we found an overexpression of XBP1 and some other genes involved in endoplasmic reticulum stress and the IRE1 branch of the unfolded protein response, as well as one co-expression module associated with these processes, which could be induced by L. infantum to prevent host cell apoptosis and modulate inflammation-induced lymphangiogenesis at lymph nodes. Moreover, 21 lncRNAs were differentially expressed in sick dogs, and one key co-expression module was associated with chromatin organization, suggesting that epigenetic mechanisms could also contribute to dampening host immune response during natural L. infantum infection in the lymph nodes of dogs suffering from clinical leishmaniosis.

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Section: Discussionmentioning

confidence: 99%

Modulation of Host Immune Response during Leishmania infantum Natural Infection: A Whole-Transcriptome Analysis of the Popliteal Lymph Nodes in Dogs

et al. 2022

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“…Further analysis of alternative splicing products from rainbow trout cath1a and cath1d genes showed that the ability to induce Il-8 in PBLs was dependent on structural flexibility in the central region, as Il-8 induction potential gradually decreased as structural flexibility was lost [ 98 ]. Atlantic salmon Cath applied to Atlantic salmon HKLs slightly induced interferon gamma (Ifn-γ), suggesting this peptide is involved in Ifn-γ-induced antigen presentation [ 102 , 103 ]. However, Il-8 stimulation could not be replicated, which might be owing to a deletion of a tandem repeat in the central region of the studied peptide compared with the previously studied variant, as this loss of structural flexibility was shown to have a significant impact on the immunomodulation ability of salmonid cathelicidins on Il-8 induction [ 24 , 103 ].…”

Section: Immunomodulatory Role Of Salmonid Ampsmentioning

confidence: 99%

“…Similar to defensins and hepcidins, the immunomodulatory functions of salmonid NK-lysins are relatively understudied. In a recent study, Acosta et al (2019) analysed whether short peptides derived from Atlantic salmon NK-lysin 1 and 2, with proposed immunomodulatory activities, had an effect on the expression of a small selection of cytokines in Atlantic salmon HKLs [ 102 ]. Stimulation with 50 µM of both peptides induced expression of pro-inflammatory il1b and il8 in HKLs [ 102 ].…”

Section: Immunomodulatory Role Of Salmonid Ampsmentioning

confidence: 99%

“…In a recent study, Acosta et al (2019) analysed whether short peptides derived from Atlantic salmon NK-lysin 1 and 2, with proposed immunomodulatory activities, had an effect on the expression of a small selection of cytokines in Atlantic salmon HKLs [ 102 ]. Stimulation with 50 µM of both peptides induced expression of pro-inflammatory il1b and il8 in HKLs [ 102 ]. These results suggest that NK-lysins might act as a signal to recruit immune cells similar to cathelicidins, as aforementioned.…”

Section: Immunomodulatory Role Of Salmonid Ampsmentioning

confidence: 99%

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Antimicrobial Peptides of Salmonid Fish: From Form to Function

Brunner

Varga

Dixon

2020

Biology

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Antimicrobial peptides (AMPs) are small, usually cationic, and amphiphilic molecules that play a crucial role in molecular and cellular host defense against pathogens, tissue damage, and infection. AMPs are present in all metazoans and several have been discovered in teleosts. Some teleosts, such as salmonids, have undergone whole genome duplication events and retained a diverse AMP repertoire. Salmonid AMPs have also been shown to possess diverse and potent antibacterial, antiviral, and antiparasitic activity and are induced by a variety of factors, including dietary components and specific molecules also known as pathogen-associated molecular patterns (PAMPs), which may activate downstream signals to initiate transcription of AMP genes. Moreover, a multitude of cell lines have been established from various salmonid species, making it possible to study host-pathogen interactions in vitro, and several of these cell lines have been shown to express various AMPs. In this review, the structure, function, transcriptional regulation, and immunomodulatory role of salmonid AMPs are highlighted in health and disease. It is important to characterize and understand how salmonid AMPs function as this may lead to a better understanding of host-pathogen interactions with implications for aquaculture and medicine.

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“…NK-lysin from Atlantic salmon ( Salmo salar ) induced the expression of proinflammatory cytokines (IL-1β and IL-8) in the S. salar head kidney leukocytes. NK-lysin modulates the immune response, suggesting its potential for enhancing the immune response in fish [64].…”

Section: Immunomodulatory Compounds From Marine Organismsmentioning

confidence: 99%

Antimicrobial and Immunomodulatory Properties and Applications of Marine-Derived Proteins and Peptides

et al. 2019

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Marine organisms provide an abundant source of potential medicines. Many of the marine-derived biomaterials have been shown to act as different mechanisms in immune responses, and in each case they can significantly control the immune system to produce effective reactions. Marine-derived proteins, peptides, and protein hydrolysates exhibit various physiologic functions, such as antimicrobial, anticancer, antioxidant, antihypertensive, and anti-inflammatory activities. Recently, the immunomodulatory properties of several antimicrobial peptides have been demonstrated. Some of these peptides directly kill bacteria and exhibit a variety of immunomodulatory activities that improve the host innate immune response and effectively eliminate infection. The properties of immunomodulatory proteins and peptides correlate with their amino acid composition, sequence, and length. Proteins and peptides with immunomodulatory properties have been tested in vitro and in vivo, and some of them have undergone different clinical and preclinical trials. This review provides a comprehensive overview of marine immunomodulatory proteins, peptides, and protein hydrolysates as well as their production, mechanisms of action, and applications in human therapy.

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In vitro immunomodulatory activities of peptides derived from Salmo salar NK-lysin and cathelicidin in fish cells

Cited by 66 publications

References 58 publications

Modulation of Host Immune Response during Leishmania infantum Natural Infection: A Whole-Transcriptome Analysis of the Popliteal Lymph Nodes in Dogs

Modulation of Host Immune Response during Leishmania infantum Natural Infection: A Whole-Transcriptome Analysis of the Popliteal Lymph Nodes in Dogs

Antimicrobial Peptides of Salmonid Fish: From Form to Function

Antimicrobial and Immunomodulatory Properties and Applications of Marine-Derived Proteins and Peptides

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