In vitro immunomodulatory activity of Lactobacillus fermentum CECT5716 and Lactobacillus salivarius CECT5713: two probiotic strains isolated from human breast milk

Pérez-Cano, Francisco J.; Dong, Honglin; Yaqoob, Parveen

doi:10.1016/j.imbio.2010.01.004

Cited by 136 publications

(116 citation statements)

References 69 publications

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“…However, there is increasing evidence that signals initiated in the intestinal lumen of a different origin (dietary functional components, changes in the microflora, the presence of microbial cell wall components, even bacterial secreted products) can interact with the intestinal mucosa and are able to regulate immune responses outside the gastrointestinal tract (16). Probiotics can suppress the progression of experimental inflammatory bowel disease, atopic dermatitis, and rheumatoid arthritis by enhancing the generation of dendritic cells and Treg in the intestinal mucosa, causing an enrichment of Treg in the inflamed regions (32,33). Similar effects were observed with plasma proteins in an experimental model of inflammatory bowel disease.…”

Section: Discussionmentioning

confidence: 99%

Dietary Plasma Proteins Modulate the Adaptive Immune Response in Mice with Acute Lung Inflammation

Maijó

Miró

Polo

et al. 2012

The Journal of Nutrition

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We examined the effects of oral plasma protein supplements on the pulmonary adaptive immune response in mice challenged with intranasal LPS. C57BL/6 mice were fed a control diet or a diet supplemented with plasma proteins [spray-dried plasma (SDP) 80 g/kg] or with an Ig concentrate [(IC) 20 g/kg] from postnatal d 19 (weaning) until d 34. Mice were challenged with PBS or LPS from Escherichia coli at d 33 and killed 24 h later for leukocyte analyses or at d 34 and killed 6 h later for cytokine determination. LPS induced the activation of T helper (Th) lymphocytes in lung and blood and this response was reduced by SDP and IC (P < 0.05). In both tissues, LPS increased the Th1 and Th2 subpopulations and this effect was inhibited by the two plasma protein supplements (P < 0.05). The LPS challenge increased the expression of all the cytokines studied (P < 0.01). SDP and IC reduced the expression of IFNγ, IL-5, IL-12p40, IL-12p70, IL-13, and IL-17 in both tissues, whereas they increased the percentage of regulatory Th lymphocytes in lung, even in PBS-treated mice (P < 0.05). LPS reduced the concentration of mature TGFβ1 (P < 0.05) in the lung but did not modify the expression of IL-10. Mice exposed to LPS and supplemented with SDP or IC showed an increased expression of the anti-inflammatory cytokine IL-10 (P < 0.05). Moreover, the two supplements increased the concentration of IL-10 in intestinal mucosa (P < 0.05). Our results show that plasma supplementation reduces the immune response that characterizes the acute lung inflammation syndrome.

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Section: Discussionmentioning

confidence: 99%

Dietary Plasma Proteins Modulate the Adaptive Immune Response in Mice with Acute Lung Inflammation

Maijó

Miró

Polo

et al. 2012

The Journal of Nutrition

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“…Moreover, a relative decrease in B fragilis was seen in patients vs controls over time 24,25 . Other commensal organisms have been shown to influence invariant natural killer T (iNKT) cell activity 26 . As both Treg cells and iNKT cells have been shown to influence diabetes onset in model systems, the ability of exogenous infectious agents, as well as commensal organisms, to influence these regulatory cell types provides mechanisms by which environmental agents might influence the host immune response.…”

Section: Environmental Factors Leading To T1dmentioning

confidence: 99%

Type 1 diabetes: translating mechanistic observations into effective clinical outcomes

et al. 2013

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Summary Type 1 diabetes remains an important health problem, particularly in Western countries where the incidence has been increasing in younger children1. In 1986, Eisenbarth described Type 1 diabetes as a chronic autoimmune disease. Work over the past 3 ½ decades has identified many of the genetic, immunologic, and environmental factors that are involved in the disease and have led to hypotheses concerning its pathogenesis. Based on these findings, clinical trials have been conducted to test these hypotheses but have had mixed results. In this review, we discuss the findings that have led to current concepts of the disease mechanisms, how this understanding has prompted clinical studies, and the results of these studies. The findings from preclinical and clinical studies support the original proposed model for how type 1 diabetes develops, but have also suggested that this disease is more complex than originally thought and will require broader treatment approaches.

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“…salivarius CECT 5713 was isolated from human milk and infant feces of a healthy mother-child pair and has been shown to be a good probiotic candidate due to its repertoire of desirable properties. This includes a high survival rate when exposed to gastrointestinal tract conditions, bile salt hydrolase activity, adherence to mucus and intestinal cells, ability to stimulate the expression of mucin-encoding genes, and in vitro and in vivo antimicrobial, anti-inflammatory, and immunomodulatory properties Olivares et al 2006;Pérez-Cano et al, 2010). Furthermore, it has been shown to be well tolerated and safe in animal models (LaraVilloslada et al 2007) and in human clinical assays (Arroyo et al 2010;Jiménez et al 2008;Maldonado et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Lactobacillus salivarius CECT 5713, a strain isolated from human milk: from genotype to phenotype

Langa

Maldonado‐Barragán

Delgado

et al. 2012

Appl Microbiol Biotechnol

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Lactobacillus salivarius CECT 5713, isolated from human milk, has immunomodulatory, anti-inflammatory and antiinfectious properties, as revealed by several in vitro and in vivo assays, which suggests a strong potential as a probiotic strain. In this work, the relationships between several genetic features of L. salivarius CECT 5713 and the corresponding phenotypes were evaluated. Although it contains a plasmid-encoded bacteriocin cluster, no bacteriocin biosynthesis was observed, possibly due to a 4-bp deletion at the beginning of the histidine kinase determinant abpK. The genome of L. salivarius CECT 5713 harbours two apparently complete prophages of 39.6 and 48 kbp. Upon induction, the 48-kbp prophage became liberated from the bacterial genome, but no DNA replication took place, which resulted in lysis of the cultures but not in phage progeny generation. The strain was sensitive to most antibiotics tested and no transmissible genes potentially involved in antibiotic resistance were detected. Finally, the genome of L. salivarius CECT 5713 contained four ORFs potentially involved in human molecular mimetism. Among them, protein 1230 was considered of particular relevance because of its similarity with dendritic cell-related proteins. Subsequently, in vitro assays revealed the ability of L. salivarius CECT 5713 to stimulate the maturation of immature dendritic cells and to inhibit the in vitro infectivity of HIV-1.

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In vitro immunomodulatory activity of Lactobacillus fermentum CECT5716 and Lactobacillus salivarius CECT5713: two probiotic strains isolated from human breast milk

Cited by 136 publications

References 69 publications

Dietary Plasma Proteins Modulate the Adaptive Immune Response in Mice with Acute Lung Inflammation

Dietary Plasma Proteins Modulate the Adaptive Immune Response in Mice with Acute Lung Inflammation

Type 1 diabetes: translating mechanistic observations into effective clinical outcomes

Characterization of Lactobacillus salivarius CECT 5713, a strain isolated from human milk: from genotype to phenotype

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