In vitro immunomodulatory effects of an oleanolic acid-enriched extract of Ligustrum lucidum fruit (Ligustrum lucidum supercritical CO2 extract) on piglet immunocytes

Wang, Jiali; Shan, Anshan; Liu, Tianyang; Zhang, Chao; Zhang, Zhiwu

doi:10.1016/j.intimp.2012.10.006

Cited by 34 publications

(24 citation statements)

References 42 publications

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“…Oleanolic acid from Prunella Vulgaris was demonstrated to induce apoptosis of lung adenocarcinoma cells through downregulating Bcl-2 expression and upregulating Bax and Bad expression (28). An oleanolic acid-enriched extract of Ligustrum Lucidum were found to have potential immunomodulatory effects through enhancing the proliferative activity of blood lymphocytes and upregulating the CD4 + CD8 + and CD4 + CD8cell populations as well as regulating the expression of Th1-and Th2-related cytokines (29). In addition, a polysaccharide from Dioscorea opposita Thunb roots was demonstrated to be able to enhance the immunological activity via stimulating ConA-induced T lymphocyte proliferation (30).…”

Section: Discussionmentioning

confidence: 99%

Fuzheng Qingjie recipe induces apoptosis in HepG2 cells via P38 MAPK activation and the mitochondria-dependent apoptotic pathway

Chen

Zhang

et al. 2014

Molecular Medicine Reports

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Fuzheng Qingjie (FZQJ) recipe is a polyherbal Chinese medicine capable of suppressing tumor growth and is used as an adjuvant therapy for various types of cancer. However, its anticancer mechanisms are yet to be fully elucidated. In the present study, we explored whether p38 mitogen-activated protein kinase (MAPK) was involved in FZQJ-mediated mitochondria-dependent apoptosis in human hepatocellular carcinoma cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays were used to measure the viability of HepG2 cells. 4,6-Diamidino-2-phenylindole (DAPI) and Annexin-V fluorescein isothiocyanate (FITC) were used to analyze the apoptosis of HepG2 cells. The mitochondrial membrane potential (∆ψ) and phosphorylated P38 MAPK protein were examined by a flow cytometer following 5,5',6,6'-tetrachloro‑1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1) and Alexa Fluor® 647 mouse anti-phosphorylated P38 MAPK antibody staining, respectively. The activation of caspase-9 and caspase-3 were measured using colorimetric assays. Additionally, Bcl-2 and Bax expression were examined using reverse transcription polymerase chain reaction (RT-PCR) and western blot analysis. The results demonstrated that water extract of FZQJ was able to induce apoptosis of HepG2 cells in vitro. FZQJ-induced apoptosis was accompanied by the loss of ∆ψ, downregulation of Bcl-2 and upregulation of Bax expression, and the activation of caspase-3, -9 and P38 MAPK. These results indicated that FZQJ induced apoptosis in HepG2 cells at least via P38 MAPK activation and the mitochondria-dependent apoptotic pathway.

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Section: Discussionmentioning

confidence: 99%

Fuzheng Qingjie recipe induces apoptosis in HepG2 cells via P38 MAPK activation and the mitochondria-dependent apoptotic pathway

Chen

Zhang

et al. 2014

Molecular Medicine Reports

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“…Splenocytes were prepared as previously [14] described and treated by nVAP32 alone at final concentrations 0, 5, 25, 50 and 100 mg/L, in presence or absence of Con A at final concentration 5 mg/L for 48 h. In order to assess only T cells, two-color analyses were performed with staining for CD4 and CD8. Briefly, staining used phycoerythrin (PE) conjugated anti-mouse L3T4 (CD4) along with FITC-conjugated anti-mouse Lyt-2 (CD8a) (BD PharMingen, USA).…”

Section: Effects Of Nvap32 On T Lymphocyte Subpopulationsmentioning

confidence: 99%

Immunomodulatory effects of a 3.2kDa polypeptide from velvet antler of Cervus nippon Temminck

Zha

et al. 2013

International Immunopharmacology

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“…Furthermore, the extract stimulated the nitric oxide (NO) secretion of lymphocytes. These results indicated that LLF-SFE might have potential immunomodulatory effects on the immune system of piglets and provided scientific and experimental foundations for the development of a new kind of LLF-SFE immune adjuvant in pig production (Wang et al 2012). …”

Section: Immunomodulatory Effectmentioning

confidence: 82%

Ligustri Lucidi Fructusas a traditional Chinese medicine: a review of its phytochemistry and pharmacology

Gao

Li²,

Wang³

et al. 2014

Natural Product Research

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Ligustri lucidi fructus (LLF) is the fruits of Ligustrum lucidum Ait. (Oleaceae). This review based on nearly 80 literary sources discusses the knowledge of chemistry and biological effects of this species. Several types of chemical constituents considered as the characteristic and active constituents from LLF were isolated including 40 triterpenoids, 48 iridoids, 10 flavones, 10 phenylethanoid glycosides and others. Various extracts and individual compounds derived from this species have been found to possess a variety of pharmacological effects, e.g. anti-tumour, hepatoprotective, immune regulating, antioxidative and anti-ageing effects, anti-inflammation and reducing hypercholesterolaemia effects and so on. The results of data analysis on the chemical, pharmacological characteristics of LLF support the view that this species has many therapeutic properties and indicate its potential as an effective herbal remedy. Finally, some suggestions for further research on chemical and pharmacological properties are given in this review. Theoretical basis was given for further exploiting and utilising LLF.

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In vitro immunomodulatory effects of an oleanolic acid-enriched extract of Ligustrum lucidum fruit (Ligustrum lucidum supercritical CO2 extract) on piglet immunocytes

Cited by 34 publications

References 42 publications

Fuzheng Qingjie recipe induces apoptosis in HepG2 cells via P38 MAPK activation and the mitochondria-dependent apoptotic pathway

Fuzheng Qingjie recipe induces apoptosis in HepG2 cells via P38 MAPK activation and the mitochondria-dependent apoptotic pathway

Immunomodulatory effects of a 3.2kDa polypeptide from velvet antler of Cervus nippon Temminck

Ligustri Lucidi Fructusas a traditional Chinese medicine: a review of its phytochemistry and pharmacology

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