In vitro, In silico and In vivo Antitumor Activity of Crude Methanolic Extract of Tetilla dactyloidea (Carter, 1869) on DEN Induced HCC in a Rat Model

Krishnan, Gowri Shankar; Rajagopal, Vidhya; Joseph, Sophy Renilda Antony; Sebastian, Divya; Ignacimuthu, S.; Selvaraj, Karthick Raja Namasivayam; Thobias, Albin Fleming

doi:10.1016/j.biopha.2017.08.054

Cited by 18 publications

(12 citation statements)

References 65 publications

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“…Conversely, the body weights of the disease control rats were significantly reduced as compared to the normal control rats. This observation can be explained by the fact that the liver is the major site for metabolism; thus, damage in liver tissues due to the hepatocarcinogenic property of the NDEA may cause loss of appetite and a decrease in adipogenesis and food ingestion, and eventually low body mass 24 . The study design and the key findings of the present study was presented in Fig.…”

Section: Discussionmentioning

confidence: 99%

Potential anti-hepatocellular carcinoma properties and mechanisms of action of clerodane diterpenes isolated from Polyalthia longifolia seeds

Tatipamula

Thonangi

Dakal

et al. 2022

Sci Rep

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Diterpenes are secondary metabolites that have attracted much attention due to their potential biological activities including anti-cancer potential. The aim of the current study is to assess the anticancer potential of the six known clerodane diterpenes (1–6) isolated from Polyalthia longifolia seeds and their underlying molecular mechanisms. These compounds were evaluated for their cytotoxicity in vitro by using MTT assays. The “two-phase model” with NDEA and PB ad libitum was used for induction of HCC and sorafenib was used as the standard drug. Prophylactic studies were carried out for compounds 4/6 at both low (5 mg/kg b.w) and high (10 mg/kg b.w) doses. Based on the MTT assay results, the two best compounds, 4 and 6, were selected for in vivo studies. The results showed that treatment with compound 4/6 significantly restored the changes in biochemical parameters and liver morphology observed in (NDEA + PB)-induced HCC rats. Additionally, the docking studies showed that compound 4/6 interacted with several key proteins such as MDM2, TNF-α, FAK, thereby inhibiting these proteins and reversing the negative impacts of NDEA. In conclusion, our results suggested that compounds 4 and 6 are potential therapeutic agents for HCC, mostly due to their ability to control typical cancer pathways.

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Section: Discussionmentioning

confidence: 99%

Potential anti-hepatocellular carcinoma properties and mechanisms of action of clerodane diterpenes isolated from Polyalthia longifolia seeds

Tatipamula

Thonangi

Dakal

et al. 2022

Sci Rep

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“…This is illustrated by the study of Steiner et al [103] in which in vivo experiments in myeloma xenografts on chicken embryos revealed significant suppression of myeloma-induced neoangiogenesis close to xenografts and a reduced revascularization of xenografts following exposure to marine compounds, even at low concentrations. Other interesting examples are the study of Krishnan et al, [104] whose investigation evaluated the anticancer activity of crude methanolic extract of Tetilla dactyloidea (CMETD) on diethylnitrosamine (DEN) induced hepatocellular carcinoma in a rat model showing a potent anti-carcinogenic effect, and the recent study of Yuan and collaborators which evaluated the antitumor potentiality of TBL-12, a new sea cucumber extract, in xenograft prostate cancer mice in vivo [105]. Anyway, once established the pharmaceutical potential of marine compounds the unavoidable following step is their chemical synthesis.…”

Section: Discussionmentioning

confidence: 99%

Bright Spots in the Darkness of Cancer: A Review of Starfishes-Derived Compounds and Their Anti-Tumor Action

et al. 2019

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The fight against cancer represents a great challenge for researchers and, for this reason, the search for new promising drugs to improve cancer treatments has become inevitable. Oceans, due to their wide diversity of marine species and environmental conditions have proven to be precious sources of potential natural drugs with active properties. As an example, in this context several studies performed on sponges, tunicates, mollusks, and soft corals have brought evidence of the interesting biological activities of the molecules derived from these species. Also, echinoderms constitute an important phylum, whose members produce a huge number of compounds with diverse biological activities. In particular, this review is the first attempt to summarize the knowledge about starfishes and their secondary metabolites that exhibited a significant anticancer effect against different human tumor cell lines. For each species of starfish, the extracted molecules, their effects, and mechanisms of action are described.

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“…The molecular mechanism of antifungal activity induction by oxidative stress has gained more attention in developing antifungal agents. Studying antioxidative responses like antioxidants (antioxidative enzymes and non-enzyme antioxidants) towards the oxidative stress induced by nanomaterials is an important criterion for utilising nanotechnology principles to ght against fungal organisms (Krishnan et al, 2017). In this present study, the level of ROS generation, antioxidative enzymes like CAT, SOD, POD, and non-enzyme antioxidant GSH in the tested fungal strain exposed to different nanocomposite dosages were investigated.…”

Section: Oxidative Stress Inductionmentioning

confidence: 98%

“…It is well known that the level of antioxidative enzymes is primarily determined by oxidative stress. Oxidative stress is a convoluted chemical and physiological spectacle that arises due to excessive production and accumulation of reactive oxygen species, which cause severe damage to the cells (Krishnan et al, 2017).…”

Section: Nanocomposite Coating On the Bread Packaged Materialsmentioning

confidence: 99%

Green chemistry principles for the synthesis of antifungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC–NT) against food spoilage fungal strain Aspergillus ochraceopealiformis

Namasivayam

Manohar

Knde

et al. 2022

Preprint

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Evaluation of potential anti fungal activity of gum acacia -gold nanocomposite fabricated with food preservative agent natamycin (GA-AuNC–NT and its noteworthy release pattern, biocompatibility was prepared in this present investigation. GA-AuNC–NT was prepared by green science principles under in vitro condition and the synthesized nanocomposite was characterized by various characterisation techniques. Green science principles that adopted in this study reveals highly stable nano dimensional composite with significant antifungal activity. The antifungal activity was investigated against the fungal strain Aspergillus ochraceopealiformis isolated from spoiled, expired bread. The well diffusion assay, fungal hyphae fragmentation assay and spore germination inhibition assay were used to determine the antifungal activity of the synthesised nanocomposite. Potential antifungal activity of the synthesised nanocomposite was confirmed by recording zone of inhibition, high rate of hyphae fragmentation and marked spore germination inhibition against the tested fungal strain. The molecular mechanism of antifungal activity was studied by measuring oxidative stress marker genes like catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) induction adopting quantitative real-time polymerase chain reaction (q RT-PCR). Among the various treatment, a notable reduction in all the tested marker genes expression was recorded in the nanocomposite treated fungal strain. Release profile studies using different solvents reveal sustained or controlled natamycin release at the increasing periods. The synthesised nanocomposite’s high safety or biocompatibility was evaluated with the Wistar animal model by determining notable changes in behavioural, biochemical, haematological and histopathological parameters. The synthesised nanocomposite did not exhibit any undesirable changes in all the tested parameters confirming the marked biosafety or biocompatibility. The nanocomposite was coated on the bread packaging material. The effect of packaging on the proximate composition, antioxidative enzymes status, and fungal growth of bread samples incubated under the incubation period were studied. Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) studies reveal that the nanocomposite was effectively coated on the packaging material without changing size, shape, and functional groups. No changes in the proximate composition and antioxidative enzymes of the packaged bread samples incubated under different incubation periods reveal the nanocomposite’s marked safety. The complete absence of the fungal growth also indicates the uniqueness of the nanocomposite. The present finding implies that the synthesised nanocomposite can be used as an effective, safe food preservative agent against food spoilage fungal strains associated with a wide range of food products and suggest a green strategy for the increased shelf life of foods.

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In vitro, In silico and In vivo Antitumor Activity of Crude Methanolic Extract of Tetilla dactyloidea (Carter, 1869) on DEN Induced HCC in a Rat Model

Cited by 18 publications

References 65 publications

Potential anti-hepatocellular carcinoma properties and mechanisms of action of clerodane diterpenes isolated from Polyalthia longifolia seeds

Potential anti-hepatocellular carcinoma properties and mechanisms of action of clerodane diterpenes isolated from Polyalthia longifolia seeds

Bright Spots in the Darkness of Cancer: A Review of Starfishes-Derived Compounds and Their Anti-Tumor Action

Green chemistry principles for the synthesis of antifungal active gum acacia-gold nanocomposite - natamycin (GA-AuNC–NT) against food spoilage fungal strain Aspergillus ochraceopealiformis

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