In vitro/in vivo characterisation of polyhedral niosomes

Arunothayanun, Parinya; Uchegbu, Ijeoma F.; Craig, Duncan Q.M.; Turton, John; Florence, Alexander T.

doi:10.1016/s0378-5173(99)00044-7

Cited by 21 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Addition of cholesterol to the formulation led to spherical vesicles that were stable on sonication under the study conditions. Cholesterol abolished the phase transition in a manner that may be analogous to that observed for liposomes (17).…”

Section: Vesicle Size and Shapementioning

confidence: 67%

Formulation and Optimization of Zidovudine Niosomes

Kandasamy¹,

2010

View full text Add to dashboard Cite

Abstract. Zidovudine (AZT) is commonly used to treat patients with AIDS, but it is limited by toxicity and high dosing needs. Alternative formulations have been proposed to overcome these drawbacks. The objective of this study was to evaluate process-related variables like hydration and sonication time, rotation speed of evaporation flask, and the effects of charge-inducing agent and centrifugation on zidovudine entrapment and release from niosomes. Formulation of zidovudine niosomes was optimized by altering the proportions of Tween, Span and cholesterol. The effect of process-related variables like hydration time, sonication time, charge-inducing agent, centrifugation and rotational speed of evaporation flask on zidovudine entrapment and release from niosomes was evaluated. The effect of changes in osmotic shock and viscosity were also evaluated. Non-sonicated niosomes were in the size range of 2-3.5 μm and sonicated niosomes formulated with Tween 80 and dicetylphosphate (DCP) had a mean diameter of 801 nm. Zidovudine niosomes formulated with Tween 80 entrapped high amounts of drug and the addition of DCP enhanced drug release for a longer time (88.72% over 12 h). The mechanism of release from Tween 80 formulation was the Fickian type and obeyed first-order release kinetics. Niosomes can be formulated by proper adjustment of process parameters to enhance zidovudine entrapment and sustainability of release. These improvements in zidovudine formulation may be useful in developing a more effective AIDS therapy.

show abstract

Section: Vesicle Size and Shapementioning

confidence: 67%

Formulation and Optimization of Zidovudine Niosomes

Kandasamy¹,

2010

View full text Add to dashboard Cite

show abstract

“…For niosome preparation, thin film hydration method was used as reported previously [41]. Specific molar ratios of Span 60, Tween 60 and cholesterol were dissolved in 10 mL of a chloroform solution containing 1 mg/ml of Lawsone and added to a 50 mL round bottom flask.…”

Section: Methodsmentioning

confidence: 99%

Lawsone-loaded Niosome and its antitumor activity in MCF-7 breast Cancer cell line: a Nano-herbal treatment for Cancer

et al. 2018

View full text Add to dashboard Cite

Phytochemicals like Lawsone have some drawbacks that stem from their poor solubility. Low solubility in aqueous mediums results in low bioavailability, poor permeability and instability of phytochemical compounds in biological environments. The aim of this study was to design nanoniosomes containing Lawsone (Law) using non-ionic surfactants and cholesterol. Niosomes were prepared by thin film hydration method (TFH). Then, they were loaded with Henna extract (HLaw) and standard Lawsone (SLaw), and two resulted formulations were compared. The henna extract was analyzed by mass gas chromatography. Size, zeta potential, polydispersity index (PDI) and morphology of the loaded formulations were evaluated by dynamic light scattering (DLS) and scanning electron spectroscopy (SEM). The incorporation and release rate of Law from niosome bilayers were evaluated by UV-Vis spectroscopy. In vitro experiments were carried out to evaluate antitumor activity in MCF-7 cell line. The results showed distinct spherical shapes and particle sizes were about 250 nm in diameter and have negative zeta potentials. Niosomes were stable at 4 °C for 2 months. Entrapment efficiently of both formulations was about 70% and showed a sustained release profile. In vitro study exhibited that using of niosome to encapsulating Law can significantly increase antitumor activity of formulation in MCF-7 cell line compared to Law solution (free Law). Thus, niosomes are a promising carrier system for delivery of phytochemical compounds that have poor solubility in biological fluids. Graphical abstract ᅟ.

show abstract

“…33 Hydration temperature is another crucial process variable in the preparation of niosomes. In order to prepare niosomes at a hydration temperature above the gel-liquid transition temperature (T c ) of the surfactants, 34 hydration was performed at 60°C, 70°C, and 80°C. The results showed that 60°C was the most suitable transition temperature.…”

Section: Resultsmentioning

confidence: 99%

Development of a novel niosomal system for oral delivery of Ginkgo biloba extract

Jin¹,

Wen²,

Garg³

et al. 2013

IJN

View full text Add to dashboard Cite

Background:The aim of this study was to develop an optimal niosomal system to deliver Ginkgo biloba extract (GbE) with improved oral bioavailability and to replace the conventional GbE tablets. Methods: In this study, the film dispersion-homogenization method was used to prepare GbE niosomes. The resulting GbE niosome suspension was freeze-dried or spray-dried to improve the stability of the niosomes. GbE-loaded niosomes were formulated and characterized in terms of their morphology, particle size, zeta potential, entrapment efficiency, and angle of repose, and differential scanning calorimetry analysis was performed. In vitro release and in vivo distribution studies were also carried out. Results: The particle size of the optimal delivery system prepared with Tween 80, Span 80, and cholesterol was about 141 nm. There was a significant difference (P , 0.05) in drug entrapment efficiency between the spray-drying method (about 77.5%) and the freeze-drying method (about 50.1%). The stability study revealed no significant change in drug entrapment efficiency for the GbE niosomes at 4°C and 25°C after 3 months. The in vitro release study suggested that GbE niosomes can prolong the release of flavonoid glycosides in phosphate-buffered solution (pH 6.8) for up to 48 hours. The in vivo distribution study showed that the flavonoid glycoside content in the heart, lung, kidney, brain, and blood of rats treated with the GbE niosome carrier system was greater than in the rats treated with the oral GbE tablet (P , 0.01). No flavonoid glycosides were detected in the brain tissue of rats given the oral GbE tablets, but they were detected in the brain tissue of rats given the GbE niosomes. Conclusion: Niosomes are a promising oral system for delivery of GbE to the brain.

show abstract

In vitro/in vivo characterisation of polyhedral niosomes

Cited by 21 publications

References 5 publications

Formulation and Optimization of Zidovudine Niosomes

Formulation and Optimization of Zidovudine Niosomes

Lawsone-loaded Niosome and its antitumor activity in MCF-7 breast Cancer cell line: a Nano-herbal treatment for Cancer

Development of a novel niosomal system for oral delivery of Ginkgo biloba extract

Contact Info

Product

Resources

About