In Vitro-In Vivo Correlation for the Antibacterial Effect of Lactiplantibacillus plantarum as a Topical Healer for Infected Burn Wound

Moraffah, Fatemeh; Kiani, Melika; Abdollahi, Mohammad; Yoosefi, Sepideh; Vatanara, Alireza; Samadi, Nasrin

doi:10.1007/s12602-022-09934-4

Cited by 7 publications

(8 citation statements)

References 46 publications

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“…By comparing the inhibition rates to these pathogens, we found that this formulation was more effective in inhibiting Gram-negative bacteria compared to Gram-positive bacteria, which is consistent with the studies by others [46]. For instance, a study on probiotics as topical therapeutic agents for infected wounds found that live probiotic cells had slightly higher inhibitory activity against P. aeruginosa than S. aureus [14]. Based on the speci city of the source of the causative strain, we hypothesize that the reason for this may be due to the fact that S. aureus isolates from clinical samples are highly drug resistant, so the honey L. plantarum formula has poor antibacterial activity against S. aureus.…”

Section: Discussionsupporting

confidence: 90%

“…L. plantarum is one of the typical representatives of lactic acid bacteria, and topical application of L. plantarum has been demonstrated to reduce or eliminate pathogenic bacterial load, reduce necrotic tissue, accelerate the appearance of granulation tissue, reduce wound area, and promote wound healing [12]. And topical administration of L. plantarum accelerated the healing of chronic diabetic foot ulcers and infected burn wounds by altering infection, angiogenesis, macrophage phenotype and neutrophil response [13,14]. It was also found that L. plantarum supernatant has protective effects against bacterial infection, oxidative stress and wound healing [15,16].…”

Section: Introductionmentioning

confidence: 99%

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Synergistic inhibitory effect of honey and Lactobacillus plantarum on pathogenic bacteria and their promotion of healing in infected wounds

Xiao

Su³

et al. 2022

Preprint

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The prevention and control of infection by resistant pathogens has become a formidable challenge due to the increasing resistance of pathogens to antibiotics, indicating the urgent need for new effective antimicrobial agents. Probiotic is a potential antibacterial drug that can address pathogenic infections. In this study, the antibacterial formulation consisting of honey and Lactobacillus plantarum (L. plantarum) was investigated the optimal antibacterial formulation, in vitro antibacterial effect and mechanism, as well as their effect on the healing of infected wounds in rat whole skin. The results of in vitro experiments showed that the optimal antibacterial formulation of honey and L. plantarum was: honey 10% + L. plantarum 1×109 CFU/mL. In vitro counts showed that the formulation inhibited more than 80% of Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa) and Escherichia coli (E. coli) after 24 h of action. In addition, live bacterial counts and growth curves indicate that L. plantarum used honey to promote its own growth and produce acids to inhibit the survival of pathogenic bacteria and biofilm formation(P < 0.001). Furthermore, in vivo results reviewed that the honey- L. plantarum formulation inhibited wound bacterial growth(P < 0.01) and increased wound epithelial granulation tissue formation to accelerate skin wound healing in a rat bacterial infection wound model. Overall, these findings suggest that the honey- L. plantarum formulation provides a new option for the treatment of pathogenic infections and wound healing, which sheds new light on the search for alternatives to antibiotics in the future.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Synergistic inhibitory effect of honey and Lactobacillus plantarum on pathogenic bacteria and their promotion of healing in infected wounds

Xiao

Su³

et al. 2022

Preprint

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“…The antibacterial potential of Lactiplantibacillus plantarum ATCC 1058 was evaluated by using in vitro simulated wound fluid (SWF) and in vivo wound models infected with two main burn wound-associated pathogens, i.e., Pseudomonas aeruginosa or Staphylococcus aureus. Results concluded both these pathogenic bacteria were susceptible to the L. plantarumproduced antimicrobial compounds and co-aggregation (115). Luan et al evaluated the antimicrobial potential of Lactobacillus curvatus BSF206 and Pediococcus pentosaceus AC1-2 against the oral pathogen Streptococcus mutans (116).…”

Section: Auto-aggregation Biofilm Formation and Co-aggregationmentioning

confidence: 99%

Probiotics, their action modality and the use of multi-omics in metamorphosis of commensal microbiota into target-based probiotics

et al. 2022

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This review article addresses the strategic formulation of human probiotics and allows the reader to walk along the journey that metamorphoses commensal microbiota into target-based probiotics. It recapitulates what are probiotics, their history, and the main mechanisms through which probiotics exert beneficial effects on the host. It articulates how a given probiotic preparation could not be all-encompassing and how each probiotic strain has its unique repertoire of functional genes. It answers what criteria should be met to formulate probiotics intended for human use, and why certain probiotics meet ill-fate in pre-clinical and clinical trials? It communicates the reasons that taint the reputation of probiotics and cause discord between the industry, medical and scientific communities. It revisits the notion of host-adapted strains carrying niche-specific genetic modifications. Lastly, this paper emphasizes the strategic development of target-based probiotics using host-adapted microbial isolates with known molecular effectors that would serve as better candidates for bioprophylactic and biotherapeutic interventions in disease-susceptible individuals.

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“…Lactobacillus plantarum ( L. plantarum ) is one of the typical representatives of lactic acid bacteria, and topical application of L. plantarum has been demonstrated to reduce or eliminate the pathogenic bacterial load, reduce necrotic tissue, accelerate the appearance of granulation tissue, reduce wound area, and promote wound healing [ 11 ]. Moreover, topical administration of L. plantarum accelerated the healing of chronic diabetic foot ulcers and infected burn wounds by altering infection, angiogenesis, macrophage phenotype, and neutrophil response [ 12 , 13 ]. It was also found that L. plantarum supernatant has protective effects against bacterial infection, oxidative stress, and wound healing [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Synergistic Inhibitory Effect of Honey and Lactobacillus plantarum on Pathogenic Bacteria and Their Promotion of Healing in Infected Wounds

Xiao

et al. 2023

Pathogens

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Prevention and control of infections have become a formidable challenge due to the increasing resistance of pathogens to antibiotics. Probiotics have been discovered to have positive effects on the host, and it is well-known that some Lactobacilli are effective in treating and preventing inflammatory and infectious diseases. In this study, we developed an antibacterial formulation consisting of honey and Lactobacillus plantarum (honey–L. plantarum). The optimal formulation of honey (10%) and L. plantarum (1 × 109 CFU/mL) was used to investigate its antimicrobial effect and mechanism in vitro, and its healing effect on wound healing of whole skin infections in rats. Biofilm crystalline violet staining and fluorescent staining results indicated that the honey–L. plantarum formulation prevented the biofilm formation in Staphylococcus aureus and Pseudomonas aeruginosa and increased the number of dead bacteria in the biofilms. Further mechanism studies revealed that the honey–L. plantarum formulation may inhibit biofilm formation by upregulating biofilm-related genes (icaA, icaR, sigB, sarA, and agrA) and downregulating quorum sensing (QS) associated genes (lasI, lasR, rhlI, rhlR, and pqsR). Furthermore, the honey–L. plantarum formulation decreased the number of bacteria in the infected wounds of rats and accelerated the formation of new connective tissue to promote wound healing. Our study suggests that the honey–L. plantarum formulation provides a promising option for the treatment of pathogenic infections and wound healing.

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In Vitro-In Vivo Correlation for the Antibacterial Effect of Lactiplantibacillus plantarum as a Topical Healer for Infected Burn Wound

Cited by 7 publications

References 46 publications

Synergistic inhibitory effect of honey and Lactobacillus plantarum on pathogenic bacteria and their promotion of healing in infected wounds

Synergistic inhibitory effect of honey and Lactobacillus plantarum on pathogenic bacteria and their promotion of healing in infected wounds

Probiotics, their action modality and the use of multi-omics in metamorphosis of commensal microbiota into target-based probiotics

Synergistic Inhibitory Effect of Honey and Lactobacillus plantarum on Pathogenic Bacteria and Their Promotion of Healing in Infected Wounds

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