In Vitro Induction and Expression of T-Cell Immunity to Tumor-Associated Antigens

Burton, Robert C.; Chism, Stanley E.; Warner, Noel L.

doi:10.1007/978-1-4684-0922-2_4

Cited by 16 publications

(11 citation statements)

References 135 publications

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“…Tbe murine tumour cell lines used in this study were: MPC-11, a PCT of BALB/c derivation; C1.18, a PCTof C3H derivation; andP-815, a mast cell tumour of DBA/2 derivation (Burton e/a/., 1975(Burton e/a/., , 1978. Tumour target cells were labelled with ^'Cr by adding 5-10 ^Ci of Na^'Cr O4 (New England Nuclear, Boston, MA.)…”

Section: Tumour Cell Linesmentioning

confidence: 99%

“…At the end of that period the BALB/c anti-MPC-U effector cells were treated in the microtrays with a 1:4 dilution of anti-Thy-1.2 (AKR ami-CBA) serum and a 1:5 dilution of rabbit complement (C), or normal mouse serum (NMS) and C, prior to the ^'Cr release assay, according to a protocol described in detail elsewhere Burton and Plate, 1981). Since culture of BALB/c spleen cells alone leads to the generation of spontaneous killer cells (Burton et al, 1978), appropriate "unstimulated' controls were included in all experiments. Statistical analysis was performed using the appropriate parametric and non-parametric tests as indicated in the text.…”

Section: Max-bgmentioning

confidence: 99%

“…1, the optimal R/S ratio was 40/1, at which there was 2O9/o specific lysis in the 6 h assay period. The R/S curve plateau at 5% specific lysis is typical of the induction of tumour specific immunity, where that 5% represents spontaneous killer cell induction (Burton et aL, 1978).…”

Section: The Responder Cell Number and Responder/stimulator Ratiomentioning

confidence: 99%

“…Therefore, clear quantitative differences are easily discernible between this phenomenon and the lysis mediated by CTL induced in vitro by PCT TAA (Burton et a!., 1978). There are, however, also qualitative differences between TAA or alloantigen-stimulated CTL and these spontaneously arising killer cells (Burton et ai, 1978).…”

Section: The Nature Of the Cytotoxic Effector Cellmentioning

confidence: 99%

“…By contrast, the frequency of CTL-P in BALB/c spleen reactive to C3H MHC and non-MHC alloantigens was 40-100 fold higher (Table 2). In this regard, it is assumed that the precursor, like the effector, is a T lymphocyte, since cultures of BALB/c nude spleen cells with MPC-11 do not yield cytotoxic effector cells (Burton et al, , 1978.…”

Section: The Frequency Of Ctl-p Reactive To Taa and Mhc Alloantigensmentioning

confidence: 99%

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Frequency of Murine Cytotoxic T Cell Precursors Reactive to Plasma Cell Tumour‐associated Antigens as Compared to Major Histocompatibility Antigens

Burton

1986

Aust J Exp Biol Med

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Summary The frequency of cytotoxic T lymphocyte precursors (CTL‐P) reactive to plasmacytoma (PCT) tumour‐associated antigens (TAA) was studied in BALB/c mouse spleen. Progressively decreasing numbers of responder BALB/c spleen cells were cultured with mitomycin C‐treated stimulator MPC‐11 BALB/c PCT cells, and a Poisson analysis was performed of the wells scored positive in a 51Cr release assay with PCT target cells. It was found that approximately 2 per million BALB/c spleen cells were CTL‐P reactive to TAA of MPC‐11, and about one in three of these precursors responded to a TAA expressed by MPC‐11 which was shared with the C3H PCT C1.18. The frequency of CTL‐P reactive to TAA was shown to be 30 to 500 fold lower than that for CTL reactive to various major histocompatibility complex alloantigens, and the addition of exogenous Interleukin‐2 only resulted in a 2‐3 fold increase in this CTL‐P frequency. These results suggest that the weak levels of tumour immunity induced in vivo by immunisation of BALB/c mice with MPC‐11 are, at least in part, due to a very low frequency of CTL‐P reactive to TAA of that PCT.

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Section: Tumour Cell Linesmentioning

confidence: 99%

Section: Max-bgmentioning

confidence: 99%

Section: The Responder Cell Number and Responder/stimulator Ratiomentioning

confidence: 99%

Section: The Nature Of the Cytotoxic Effector Cellmentioning

confidence: 99%

Section: The Frequency Of Ctl-p Reactive To Taa and Mhc Alloantigensmentioning

confidence: 99%

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Frequency of Murine Cytotoxic T Cell Precursors Reactive to Plasma Cell Tumour‐associated Antigens as Compared to Major Histocompatibility Antigens

Burton

1986

Aust J Exp Biol Med

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Tumor immunity to murine plasma‐cell tumors. VIII. Immunosuppression of the generation of cytotoxic T cells by murine plasma‐cell tumor lines

Giorgi

Warner

1983

Intl Journal of Cancer

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Previous studies have clearly established that murine plasmacytomas suppress B-cell responses, but no effects on T-cell responses have been noted by most investigators. However, in our investigation of the tumor-associated antigens of plasmacytomas, we have found that immunosuppression plays an important role in the complex interaction between host immune T cells and tumor cells. In these investigations, varying the number of plasmacytoma cells added to in vitro induction systems separated two different effects which these cells had on the generation of cytotoxic T cells. On the one hand, when appropriate numbers were present, tumor cells acted as immunogens and stimulated the generation of specific killer cells against tumor-associated antigen or alloantigen which they expressed. In contrast, greater numbers of tumor cells exerted a profound inhibitory effect on the generation of cytotoxic activity when cells inactivated with irradiation were used. Mitomycin-C inactivation of plasmacytoma cells abrogated this inhibitory activity. In further experiments, MPC-II cells that had been inactivated with Mitomycin C were able to prime T cells in vivo, whereas priming by live tumor cells could not be detected in the same situation. It is suggested that immunosuppression by the live tumor cells may account for their inability to prime T cells in vivo. These results indicate that immunosuppression by plasmacytomas may be one of the important variables which influence the generation of cytotoxic T cells against tumor-associated antigens both in vivo and in vitro.

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The Generation and Down-Regulation of the Immune Response to Progressive Tumors

North

Digiacomo

Dye

1987

Development and Recognition of the Transformed Cell

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In Vitro Induction and Expression of T-Cell Immunity to Tumor-Associated Antigens

Cited by 16 publications

References 135 publications

Frequency of Murine Cytotoxic T Cell Precursors Reactive to Plasma Cell Tumour‐associated Antigens as Compared to Major Histocompatibility Antigens

Frequency of Murine Cytotoxic T Cell Precursors Reactive to Plasma Cell Tumour‐associated Antigens as Compared to Major Histocompatibility Antigens

Tumor immunity to murine plasma‐cell tumors. VIII. Immunosuppression of the generation of cytotoxic T cells by murine plasma‐cell tumor lines

The Generation and Down-Regulation of the Immune Response to Progressive Tumors

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